Recombinant Human FGF-10 Heat Stable Protein, CF

R&D Systems | Catalog # BT-FGF10HS

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human FGF-10 Heat Stable Protein (BT-FGF10HS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Applications

Bioactivity
View all FGF-10 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human FGF-10 protein
Prorietary, engineered based on O15520.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

SDS-PAGE

19-23 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb.
The ED50 for this effect is 4.00-60.0 ng/mL.

Scientific Data Images for Recombinant Human FGF-10 Heat Stable Protein, CF

Recombinant Human FGF‑10 Heat Stable Protein Bioactivity.

Bioactivity of Recombinant Human FGF-10 Heat Stable Protein (Catalog # BT-FGF10HS) was measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED50 for this effect is 4.00-60.0 ng/mL.

Recombinant Human FGF‑10 Heat Stable Bioactivity.

Recombinant Human FGF‑10 protein induces proliferation of Ba/F3 mouse pro-B cells transfected with human FGFR2b. (A) Recombinant Human FGF‑10 Heat Stable (Catalog # BT-FGF10HS) or (B) Recombinant Human FGF‑10 (345-FG) were either untreated or incubated at 37°C for 9 days in media. The heat-stable (HS) FGF‑10 retained similar bioactivity after incubation compared to the untreated HS protein, indicating that the HS protein has increased thermal stability. In contrast, the wild-type (WT) FGF‑10 protein showed a significant loss of activity following incubation, suggesting less thermal stability.

Recombinant Human FGF‑10 Heat Stable Protein SDS-PAGE.

2 μg/lane of Recombinant Human FGF‑10 Heat Stable Protein (Catalog # BT-FGF10HS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19-23 kDa, under reducing conditions.

Heat Stable FGF-10 maintains activity at 48hr and 72 hrs at 37°C where Wild Type FGF-10 loses activity.

To evaluate the signal transduction response triggered by FGF-10, a HEK293 cell line that expresses a reporter for SEAP controlled by the Serum Response Element was utilized. Wild type FGF-10 (345-FG) and Heat Stable FGF-10HS (BT-FGF10HS) were incubated in DMEM media at 37°C for 48 or 72 hrs at concentrations of 600 ng/mL. Specific activity was assessed through a SEAP bioassay in HEK293 cells, wherein cells were treated with FGF-10 WT or FGF-10HS for 24 hours. The specific activity % of control was determined, with the control comprising FGF-10 stored at 4°C without any incubation.

Recombinant Human FGF-10 Heat Stable SPR

Recombinant Human FGF R2 beta (IIIb) (Catalog # 665-FR) was captured on Biacore Sensor Chip CM5 via Recombinant Protein A/G/L (Catalog # NBP2-34985), and binding to Recombinant Human FGF 10 Heat Stable (Catalog # BT-FGF10HS) was measured at a concentration range between 0.0488 nM and 12.5 nM. The double-referenced sensorgram was fit to a two-state reaction binding model to determine the binding kinetics and affinity, with an affinity constant of KD=36.5 pM. (Biacore T200).

Recombinant Human FGF-10 SPR

Recombinant Human FGF R2 beta (IIIb) (Catalog # 665-FR) was captured on Biacore Sensor Chip CM5 via Recombinant Protein A/G/L (Catalog # NBP2-34985), and binding to Recombinant Human FGF 10 (Catalog # 345-FG) was measured at a concentration range between 0.0488 nM and 12.5 nM. The double-referenced sensorgram was fit to a two-state reaction binding model to determine the binding kinetics and affinity, with an affinity constant of KD=36.2 pM. (Biacore T200).

Heat Stable FGF-10 Supports Organoid Growth Equivalent to Wild Type in Basement Membrane Extract.

Adult airway organoids were cultured in Cultrex UltiMatrix (Catalog # BME001-05) with either Recombinant Human FGF-10 Heat Stable Protein (HS, Catalog # BT-FGF10HS) or wild type Recombinant Human FGF-10 (WT, Catalog # 345-FG). FGF-10 Heat Stable demonstrates that it can support organoid growth as effectively as wild type in an extracellular matrix. Scale bar indicates 1 mm.

Heat Stable Protein Supports Organoid Growth Equivalent to Wild Type in a Defined Matrix.

Adult airway organoids were cultured in Cultrex Synthetic Hydrogel (Catalog # CSH-RUO-01) with either Recombinant Human FGF-10 Heat Stable Protein (HS, Catalog # BT-FGF10HS) or wild type Recombinant Human FGF-10 Protein (WT, Catalog # 345-FG). FGF-10 Heat Stable Protein demonstrates that it can support organoid growth as effectively as wild type in a defined animal-free extracellular matrix. Scale bar indicates 1 mm.

Formulation, Preparation, and Storage

BT-FGF10HS
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-10

Fibroblast Growth Factors including FGF-10 are known to be intrinsically unstable, and prone to unfold at 37°C, quickly rendering the growth factor inactive. Our de novo Heat Stable FGF-10 has been engineered for thermostability to maintain activity at 37℃ for at least 15 days. Heat-stable FGF-10 can enhance the consistency and efficacy of cell culture or organoid development. Heat Stable FGF-10 maintains sequence similarity, with >95% sequence homology to native FGF-10, making FGF-10 Heat Stable growth factor an easy replacement for culture.

Long Name

Fibroblast Growth Factor 10

Alternate Names

FGF10, KGF-2, KGF2

Entrez Gene IDs

2255 (Human); 14165 (Mouse); 25443 (Rat)

Gene Symbol

FGF10

Additional FGF-10 Products

Product Documents for Recombinant Human FGF-10 Heat Stable Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human FGF-10 Heat Stable Protein, CF

For research use only

Related Research Areas

FGF Family
FGF Ligands
gamma delta T Cells

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