Recombinant Human FGF-10 Protein 345-FG-025: R&D Systems

Recombinant Human FGF-10 Protein

(37 citations)   
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 20-100 ng/mL.
  • Source
    E. coli-derived Cys37-Ser208 & Gly41-Ser208
  • Accession #
  • N-terminal Sequence
    Analysis
    Cys37 & Gly41
  • Predicted Molecular Mass
    19.5 kDa
  • SDS-PAGE
    19-22 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
345-FG
 
345-FG/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human FGF-10 (Catalog # 345-FG) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50 for this effect is 20‑100 ng/mL.
1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.
Background: FGF-10
The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (8, 9), formation of the otic vesicle and chochlea (10), adipogenesis (11), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (12 - 15). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 10, 13 ‑ 15). The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung (8, 9). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF‑10 biology (7, 10, 14, 15). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (16).
  • References:
    1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
    2. Igarashi, M. et al. (1998) J. Biol. Chem. 273:13230.
    3. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
    4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
    5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
    6. Min, H. et al. (1998) Genes Dev. 12:3156.
    7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
    8. Bellusci, S. et al. (1997) Development 124:4867.
    9. Weaver, M. et al. (2000) Development 127:2695.
    10. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
    11. Sakaue, H. et al. (2002) Genes Dev. 16:908.
    12. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
    13. Mailleux, A.A. et al. (2002) Development 129:53.
    14. Makarenkova, H.P. et al. (2000) Development 127:2563.
    15. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
    16. Nomura, S. et al. (2008) Br. J. Cancer 99:305.
  • Long Name:
    Fibroblast Growth Factor 10
  • Entrez Gene IDs:
    2255 (Human); 14165 (Mouse); 25443 (Rat)
  • Alternate Names:
    FGF10; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; KGF-2; produced by fibroblasts of urinary bladder lamina propria
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling
    Authors: KB McCauley, F Hawkins, M Serra, DC Thomas, A Jacob, DN Kotton
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Lung Basal Stem Cells Rapidly Repair DNA Damage Using the Error-Prone Nonhomologous End-Joining Pathway
    Authors: CE Weeden, Y Chen, SB Ma, Y Hu, G Ramm, KD Sutherland, GK Smyth, ML Asselin-La
    PLoS Biol, 2017;15(1):e2000731.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. ?E-Catenin Is a Positive Regulator of Pancreatic Islet Cell Lineage Differentiation
    Authors: AJ Jimenez-Ca, R Pillich, W Yang, GR Diaferia, P Meda, L Crisa, V Cirulli
    Cell Rep, 2017;20(6):1295-1306.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Transcription factor Etv5 is essential for the maintenance of alveolar type II cells
    Authors: Z Zhang, K Newton, SK Kummerfeld, J Webster, DS Kirkpatric, L Phu, J Eastham-An, J Liu, WP Lee, J Wu, H Li, MR Junttila, VM Dixit
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Bioassay
  5. Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
    Authors: KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro
    Nat Commun, 2017;8(1):331.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts
    Authors: CR Ball, F Oppel, KR Ehrenberg, TD Dubash, SM Dieter, CM Hoffmann, U Abel, F Herbst, M Koch, J Werner, F Bergmann, N Ishaque, M Schmidt, C von Kalle, C Scholl, S Fröhling, B Brors, W Weichert, J Weitz, H Glimm
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: Bioassay
  7. Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration
    Stem Cells Transl Med, 2016;5(5):694-702.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Retinal Proteoglycans Act as Cellular Receptors for Basement Membrane Assembly to Control Astrocyte Migration and Angiogenesis
    Cell Rep, 2016;17(7):1832-1844.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Binding Assay
  9. TGF-?1 promotes acinar to ductal metaplasia of human pancreatic acinar cells
    Sci Rep, 2016;6(0):30904.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Photodynamic therapy inhibit Fibroblast Growth Factor-10 induced keratinocyte differentiation and proliferation through ROS in Fibroblast Growth Factor Receptor-2b pathway
    Authors: Maya Valeska Gozali
    Sci Rep, 2016;6(0):27402.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Isolation and characterization of embryonic stem cell-derived cardiac Purkinje cells.
    Authors: Maass K, Shekhar A, Lu J, Kang G, See F, Kim E, Delgado C, Shen S, Cohen L, Fishman G
    Stem Cells, 2015;33(4):1102-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. Unliganded fibroblast growth factor receptor 1 forms density-independent dimers.
    Authors: Comps-Agrar L, Dunshee D, Eaton D, Sonoda J
    J Biol Chem, 2015;290(40):24166-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure.
    Authors: Calderon-Gierszal E, Prins G
    PLoS ONE, 2015;10(7):e0133238.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.
    Authors: Huang S, Green M, de Carvalho A, Mumau M, Chen Y, D'Souza S, Snoeck H
    Nat Protoc, 2015;10(3):413-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
    Authors: Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet
    Nat Protoc, 2015;10(3):363-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.
    Authors: Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor A, Yasuda Y, Niki T
    BMC Cancer, 2015;15(0):82.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Recessive mutations in a distal PTF1A enhancer cause isolated pancreatic agenesis.
    Authors: Weedon M, Cebola I, Patch A, Flanagan S, De Franco E, Caswell R, Rodriguez-Segui S, Shaw-Smith C, Cho C, Lango Allen H, Houghton J, Roth C, Chen R, Hussain K, Marsh P, Vallier L, Murray A, Ellard S, Ferrer J, Hattersley A
    Nat Genet, 2014;46(1):61-4.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Human marrow stromal cells downsize the stem cell fraction of lung cancers by fibroblast growth factor 10.
    Authors: Kanehira M, Kikuchi T, Santoso A, Tode N, Hirano T, Ohkouchi S, Tamada T, Sugiura H, Harigae H, Ichinose M
    Mol Cell Biol, 2014;34(15):2848-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
    Authors: Takeuchi, Hiroki, Nakatsuji, Norio, Suemori, Hirofumi
    Sci Rep, 2014;4(0):4488.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Polycomb group protein expression during differentiation of human embryonic stem cells into pancreatic lineage in vitro.
    Authors: Pethe, Prasad, Nagvenkar, Punam, Bhartiya, Deepa
    BMC Cell Biol, 2014;15(0):18.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.
    Authors: Huang, Sarah X, Islam, Mohammad, O'Neill, John, Hu, Zheng, Yang, Yong-Gua, Chen, Ya-Wen, Mumau, Melanie, Green, Michael, Vunjak-Novakovic, Gordana, Bhattacharya, Jahar, Snoeck, Hans-Wil
    Nat Biotechnol, 2014;32(1):84-91.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  22. Leading and trailing cells cooperate in collective migration of the zebrafish posterior lateral line primordium.
    Authors: Dalle Nogare D, Somers K, Rao S, Matsuda M, Reichman-Fried M, Raz E, Chitnis A
    Development, 2014;141(16):3188-96.
    Species: Zebrafish
    Sample Type: Whole Organism
    Application: In Vivo
  23. Enzyme-free passage of human pluripotent stem cells by controlling divalent cations.
    Authors: Ohnuma, Kiyoshi, Fujiki, Ayaka, Yanagihara, Kana, Tachikawa, Saoko, Hayashi, Yohei, Ito, Yuzuru, Onuma, Yasuko, Chan, Techuan, Michiue, Tatsuo, Furue, Miho K, Asashima, Makoto
    Sci Rep, 2014;4(0):4646.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Generation of multipotent foregut stem cells from human pluripotent stem cells.
    Authors: Hannan, Nicholas, Fordham, Robert P, Syed, Yasir A, Moignard, Victoria, Berry, Andrew, Bautista, Ruben, Hanley, Neil A, Jensen, Kim B, Vallier, Ludovic
    Stem Cell Reports, 2013;1(4):293-306.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2013;21(1):228-39.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
    Authors: Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, Markmann J, Miyazaki S, Miyazaki J, Szot G, Bottino R, Kim S
    Elife, 2013;2(0):e00940.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. A molecular signature for purified definitive endoderm guides differentiation and isolation of endoderm from mouse and human embryonic stem cells.
    Authors: Wang P, McKnight K, Wong D, Rodriguez R, Sugiyama T, Gu X, Ghodasara A, Qu K, Chang H, Kim S
    Stem Cells Dev, 2012;21(12):2273-87.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
    Cell Transplant, 2012;21(6):1321-39.
    Species: Human
    Sample Type: Whole Cells
    Application: cell culture
  29. Coordination of epithelial branching and salivary gland lumen formation by Wnt and FGF signals.
    Authors: Patel N, Sharpe PT, Miletich I
    Dev. Biol., 2011;358(1):156-67.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  30. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
    Authors: Tomlinson DC, Knowles MA
    Am. J. Pathol., 2010;177(5):2379-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells.
    Authors: Borowiak M, Maehr R, Chen S, Chen AE, Tang W, Fox JL, Schreiber SL, Melton DA
    Cell Stem Cell, 2009;4(4):348-58.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  32. Specific heparan sulfate structures modulate FGF10-mediated submandibular gland epithelial morphogenesis and differentiation.
    Authors: Patel VN, Likar KM, Zisman-Rozen S, Cowherd SN, Lassiter KS, Sher I, Yates EA, Turnbull JE, Ron D, Hoffman MP
    J. Biol. Chem., 2008;283(14):9308-17.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  33. Platelet-derived growth factor receptor regulates salivary gland morphogenesis via fibroblast growth factor expression.
    Authors: Yamamoto S, Fukumoto E, Yoshizaki K, Iwamoto T, Yamada A, Tanaka K, Suzuki H, Aizawa S, Arakaki M, Yuasa K, Oka K, Chai Y, Nonaka K, Fukumoto S
    J. Biol. Chem., 2008;283(34):23139-49.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  34. Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells.
    Authors: Cho YM, Lim JM, Yoo DH, Kim JH, Chung SS, Park SG, Kim TH, Oh SK, Choi YM, Moon SY, Park KS, Lee HK
    Biochem. Biophys. Res. Commun., 2007;366(1):129-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. Heparanase cleavage of perlecan heparan sulfate modulates FGF10 activity during ex vivo submandibular gland branching morphogenesis.
    Authors: Patel VN, Knox SM, Likar KM, Lathrop CA, Hossain R, Eftekhari S, Whitelock JM, Elkin M, Vlodavsky I, Hoffman MP
    Development, 2007;134(23):4177-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  36. FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
    Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
    Development, 2005;132(6):1223-34.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  37. Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein.
    Authors: Mogi K, Goto M, Ohno E, Azumi Y, Takeuchi S, Toyoizumi R
    Int. J. Dev. Biol., 2003;47(1):15-29.
    Species: Xenopus
    Sample Type: In Vivo
    Application: In Vivo
Expand to show all 37 Citations

Have you used Recombinant Human FGF-10 Protein?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

  • Formulations

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information