Recombinant Human FGF R1 beta (IIIc) Fc Chimera Protein, CF

(7 citations)   
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 1‑3 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Human FGF R1 beta (IIIc)
    (Arg22-Glu285)
    Accession # NP_075594
    IEGRDMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg22
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    56 kDa (monomer)
  • SDS-PAGE
    90-95 kDa, reducing conditions
661-FR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images

Recombinant Human FGF R1 beta (IIIc) (Catalog # 661-FR) inhibits FGF acidic-dependent cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 1-3 ng/mL.

1 μg/lane of Recombinant Human FGF R1 beta (IIIc) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 90 kDa and 180 kDa, respectively.

Background: FGF R1 beta

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1-3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform. Only the alpha  isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1 - 3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1 - 3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

  • References:
    1. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
    2. Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.
  • Long Name:
    Fibroblast Growth Factor Receptor 1 beta
  • Entrez Gene IDs:
    2260 (Human)
  • Alternate Names:
    FGF R1 beta; FGFR1b
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Expression, refolding and spectroscopic characterization of fibronectin type III (FnIII)-homology domains derived from human fibronectin leucine rich transmembrane protein (FLRT)-1, -2, and -3
    Authors: L Yang, M Hansen Fal, PW Thulstrup, PS Walmod, L Lo Leggio, K Krighaar R
    PeerJ, 2017;5(0):e3550.
    Species: Human
    Sample Type: Recombinant Proteins
    Application: Bioassay
  2. Monoclonal antibody targeting of fibroblast growth factor receptor 1c ameliorates obesity and glucose intolerance via central mechanisms.
    Authors: Lelliott C, Ahnmark A, Admyre T, Ahlstedt I, Irving L, Keyes F, Patterson L, Mumphrey M, Bjursell M, Gorman T, Bohlooly-Y M, Buchanan A, Harrison P, Vaughan T, Berthoud H, Linden D
    PLoS ONE, 2014;9(11):e112109.
    Species: Human
    Sample Type: Protein
    Application: Binding Assay
  3. Selection and characterization of a human neutralizing antibody to human fibroblast growth factor-2.
    Authors: Tao J, Xiang JJ, Li D, Deng N, Wang H, Gong YP
    Biochem. Biophys. Res. Commun., 2010;394(3):767-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. A specific survival response in dopamine neurons at most risk in Parkinson's disease.
    Authors: Murase S, McKay RD
    J. Neurosci., 2006;26(38):9750-60.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  5. FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc.
    Authors: Nakayama T, Mutsuga N, Tosato G
    Blood, 2006;109(4):1363-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
    Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
    Development, 2005;132(6):1223-34.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Cooperation between sonic hedgehog and fibroblast growth factor/MAPK signalling pathways in neocortical precursors.
    Authors: Kessaris N, Jamen F, Rubin LL, Richardson WD
    Development, 2004;131(6):1289-98.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

BA 110-HG 66
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