Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive Uev1a/UBE2V1 protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. Human Uev1a/UBE2V1 shares 89% aa sequence identity with its mouse and rat orthologs. The UBE2N (Ubc13)/Uev1a Complex functions with Ubiquitin ligases (E3s), including TRAF-6 and TRIM5, to synthesize Lys63-linked Ubiquitin chains (2,3). These Ubiquitin chains can either be unanchored or attached to target proteins (2,4). The UBE2N (Ubc13)/Uev1a Complex localizes primarily to the cytoplasm and is important for NF-kappa B activation (5). Additionally, this complex may function in the intracellular response to retroviral capsid recognition (3). Pathologically, the UBE2N (Ubc13)/Uev1a Complex is required for the proliferation of diffuse large B-cell lymphoma cells, suggesting that it may play a role in cancer (6).
Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF
R&D Systems | Catalog # E2-664
Discontinued Product
E2-664 has been discontinued.
View all UBE2N (Ubc13)/Uev1a Complex products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human UBE2N (Ubc13)/Uev1a Complex protein
Contains an terminal 6-His tag
Contains an terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
18 kDa (UBE2N) & 21 kDa (Uev1a)
Activity
Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex concentration of 0.1-1 μM.
Formulation, Preparation, and Storage
E2-664
| Formulation | X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM Nacl, 10% Glycerol (v/v), 2 mM TCEP. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UBE2N (Ubc13)/Uev1a Complex
References
- Deng, L. et al. (2000) Cell 103:351.
- Xia, Z.P. et al. (2009) Nature 461:114.
- Pertel, T. et al. (2011) Nature 472:361.
- Liu, C. et al. (2009) Sci. Signal. 2:ra63.
- Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.
- Pulvino, M. et al. (2012) Blood 120:1668.
Entrez Gene IDs
7334 (Human)
Gene Symbol
UBE2N
UniProt
Product Documents for Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF
Certificate of Analysis
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Product Specific Notices for Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF
For research use only
Related Research Areas
Citations for Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF
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