Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF

Recombinant Human IL-1 beta/IL-1F2 GMP Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

• Designed, manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
• Documented and controlled manufacturing process
• Control of documentation and process changes by QA
• Personnel training programs
• Raw material inspection and vendor qualification/monitoring program
• Validated equipment, processes and test methods
• Equipment calibration and maintenance schedules using a Regulatory Asset Manager
• Facility/Utilities maintenance, contamination controls, safety and pest control programs
• Material review process for variances
• Robust product stability program following relevant ICH guidelines
  
  

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their application. Each product is provided with a lot-specific Certificate of Analysis that contains the product's specifications and test results. Quality control testing may include, but is not limited to:

• N-terminal amino acid analysis
• SDS-PAGE purity analysis
• Molecular weight analysis via mass spectrometry
• Endotoxin assessment per USP <85> and Ph. Eur. 2.6.14 guidelines
• Bioassay analysis
• Microbial testing per USP <71> and Ph. Eur. 2.6.1 guidelines
• Host cell protein assessment
• Host cell DNA assessment
• Mycoplasma assessment
  
  

Additional testing and documentation requested by the customer can be arranged at an additional cost. 

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis and St. Paul, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.


Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

• All molecular biology procedures use animal-free media and dedicated labware.
• Dedicated fermentors are utilized in committed animal-free areas.
  
  

Purification

• Protein purification columns are animal-free.
• Bulk proteins are filtered using animal-free filters.
• Purified proteins are stored in animal-free containers.

Please read our complete Animal-Free Statement

Product Specific Notices

Full terms and conditions of sale can be found online in the Protein Sciences Segment T&Cs at: This product is covered by one or more of the following U.S. patents: 5,681,933 and5,474,899.

Related Research Areas

• Alpha Granule Soluble Molecules
• Cancer Biomarkers
• Conventional/Classical Dendritic Cells
• Cytokines and Receptors in Allergy and Asthma
• Cytokines and Receptors in Angiogenesis
• IL-1 Family
• Infectious Diseases
• Inflammatory Mediators
• Isolation, Culture, and Identification of Natural Killer Cells
• LTi Cells
• Macrophage Activation Markers
• Microglia Activation Markers
• Multiple Sclerosis and Myelin Related Molecules
• Myeloid-derived Suppressor Cells (MDSC)
• NCR-ILC3
• Neuroinflammatory Factors
• NOD-like Receptors and the Inflammasome
• Regulatory T Cells (Tregs)
• Search Cytokine and Growth Factor Products
• T Cell Cytokine Signaling
• Th17 Cells
• Th9 Cells