Interleukin 15 Receptor alpha (IL‑15 R), also known as CD215, is a widely expressed 60 kDa transmembrane glycoprotein that plays an important role in the homeostasis and activation of NK cells and CD8+ memory T cells and participates in the development and function of many other hematopoietic cell types and non‑immune cell types (1 ‑ 3). Mature human IL‑15 R alpha consists of a 175 aa extracellular domain (ECD) containing one N‑linked glycosylation site, a 23 aa transmembrane segment, and a 39 aa cytoplasmic tail (4). Within the ECD, human IL‑15 R alpha shares approximately 60% aa sequence identity with mouse and rat IL‑15 R alpha. Alternate splicing of human IL‑15 R alpha generates additional isoforms with variable length deletions in the ECD and/or substitutions in the cytoplasmic domain (4, 5). IL‑15 R alpha binds to Interleukin‑15 with high affinity (6). IL‑15 additionally interacts with lower affinity to a complex of IL‑2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL‑2 receptor complex (7, 8). The use of shared receptor components contributes to the overlapping biological effects of IL‑15 and IL‑2. The dominant mechanism of IL‑15 action is known as transpresentation in which IL‑15/IL‑15 R alpha complexes are expressed on the surface of one cell and interact with complexes of IL‑2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL‑15 even if they do not express IL‑15 R alpha (10 ‑ 12). IL‑15/IL‑15 R alpha complexes can transmit reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL‑15/IL‑15 R alpha expressing cells (13, 14). Shed soluble forms of IL‑15 R alpha retain the ability to bind tightly to IL‑15 and can inhibit IL‑15 bioactivity (6, 15, 16).
Recombinant Human IL-15R alpha Fc Chimera Protein, CF
R&D Systems | Catalog # 147-IR
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Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant Human IL-15R alpha Fc Chimera Protein (147-IR)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Sf 21 (baculovirus)
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-15 R alpha protein
| Human IL-15 R alpha (Ile31-Thr172) Accession # EAW86418 |
IEGRDMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile31
Predicted Molecular Mass
42.6 kDa (monomer)
SDS-PAGE
51-64 kDa, reducing conditions
Activity
Measured by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells. Ruchatz, H. et al. (1998) J. Immunol. 160:5654.
The ED50 for this effect is 0.005‑0.015 µg/mL.
The ED50 for this effect is 0.005‑0.015 µg/mL.
Reviewed Applications
Read 3 reviews rated 4.7 using 147-IR in the following applications:
Formulation, Preparation, and Storage
147-IR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: IL-15R alpha
References
- Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
- Di Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
- Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
- Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
- Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
- Giri, J.G. et al. (1995) EMBO 14:3654.
- Grabstein, K. et al. (1994) Science 264:965.
- Giri, J. et al. (1994) EMBO J. 13:2822.
- Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
- Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
- Dubois, S. et al. (2002) Immunity 17:537.
- Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
- Neely, G.G. et al. (2004) J. Immunol. 172:4225.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
- Mortier, E. et al. (2004) J. Immunol. 173:1681.
Long Name
Interleukin 15 Receptor alpha
Alternate Names
CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA
Gene Symbol
IL15RA
UniProt
Additional IL-15R alpha Products
Product Documents for Recombinant Human IL-15R alpha Fc Chimera Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human IL-15R alpha Fc Chimera Protein, CF
For research use only
Citations for Recombinant Human IL-15R alpha Fc Chimera Protein, CF
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Customer Reviews for Recombinant Human IL-15R alpha Fc Chimera Protein, CF (3)
4.7 out of 5
3 Customer Ratings
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Application: ELISA standardVerified Customer | Posted 06/09/2020We used this protein in an in-house ELISA along with mAb and pAb (AF247) to quantify IL-15R alpha in human serum and plasma. This combination could not detect IL-15R alpha in our samples but generated a good standard curve.
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 05/07/2019
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Application: Apoptosis assayVerified Customer | Posted 04/05/2019
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