Recombinant Human INSRR Protein, CF

R&D Systems | Catalog # 9566-RR

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human INSRR Protein (9566-RR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P14616

Structure / Form

Disulfide-linked heterotetramer
View all INSRR Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human INSRR protein
  
Human INSRR beta
(Ala747-Leu923)
Accession # P14616		 HHHHHH
Human INSRR alpha
(Leu24-Gly742)
Accession # P14616
Human INSRR alpha
(Leu24-Gly742)
Accession # P14616
Human INSRR beta
(Ala747-Leu923)
Accession # P14616		 HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24 (INSRR alpha ) & Ala747 (INSRR beta )

Predicted Molecular Mass

100 kDa

SDS-PAGE

30-39 kDa, 93 - 116 kDa, 124 - 144 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9566-RR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: INSRR

Insulin Receptor-related Receptor (INSRR) is a type I transmembrane glycoprotein in the Insulin/IGF receptor family of receptor tyrosine kinases (1). The mature receptor contains an extracellular alpha subunit and an extracellular/transmembrane/cytoplasmic beta subunit. The amino acid 1-923 encodes the extracellular domain. Alternative splicing generates A and B isoforms which may homodimerize or heterodimerize with the IGF-I receptor (2). INSRR signaling regulates glucose uptake and metabolism but also contributes to cell growth, differentiation and apoptosis. INSRR is specifically and reversibly activated by alkaline media through a positive cooperativity mechanism. It also triggers receptor conformational changes and elicits intracellular signaling. INSRR is predominantly expressed in organs that come in contact with mildly alkaline media, and is postulated to be an alkali sensor to manage metabolic bicarbonate excess in kidney (3). Mutations in INSRR have been linked to severe insulin resistance (type A and Rabson-Mendenhall syndrome), type II diabetes mellitus, and leprechaunism (Donohue syndrome) (4, 5). Human INSRR shares 89% identity with mouse and rat INSRR.

References

  1. Nef, S. et al. (2003) Nature 426:291.
  2. Lee, J. & Pilch, P. F. (1994) Am J Physiol Cell Physiol. 266:C319.
  3. Deyev, I. E. et al. (2011) Cell Metab. 13:679.
  4. Kobayashi, M. et al. (1978). Proc Natl Acad Sci U.S.A. 75:3469.
  5. Musso, C. et al. (2004). Medicine 83:209.

Long Name

Insulin Receptor-related Receptor

Alternate Names

IRR

Entrez Gene IDs

3645 (Human); 23920 (Mouse)

Gene Symbol

INSRR

UniProt

P14616

Additional INSRR Products

Product Documents for Recombinant Human INSRR Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human INSRR Protein, CF

For research use only

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