Recombinant Human INSRR Protein, CF

Catalog # Availability Size / Price Qty
9566-RR-050
The Innovator Series.
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Recombinant Human INSRR Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Chinese Hamster Ovary cell line, CHO-derived human INSRR protein
  
Human INSRR beta
(Ala747-Leu923)
Accession # P14616
HHHHHH
Human INSRR alpha
(Leu24-Gly742)
Accession # P14616
Human INSRR alpha
(Leu24-Gly742)
Accession # P14616
Human INSRR beta
(Ala747-Leu923)
Accession # P14616
HHHHHH
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Leu24 (INSRR alpha ) & Ala747 (INSRR beta )
Structure / Form
Disulfide-linked heterotetramer
Predicted Molecular Mass
100 kDa
SDS-PAGE
30-39 kDa, 93 - 116 kDa, 124 - 144 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9566-RR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity not tested The Innovator Series. View Larger

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: INSRR

Insulin Receptor-related Receptor (INSRR) is a type I transmembrane glycoprotein in the Insulin/IGF receptor family of receptor tyrosine kinases (1). The mature receptor contains an extracellular alpha subunit and an extracellular/transmembrane/cytoplasmic beta subunit. The amino acid 1-923 encodes the extracellular domain. Alternative splicing generates A and B isoforms which may homodimerize or heterodimerize with the IGF-I receptor (2). INSRR signaling regulates glucose uptake and metabolism but also contributes to cell growth, differentiation and apoptosis. INSRR is specifically and reversibly activated by alkaline media through a positive cooperativity mechanism. It also triggers receptor conformational changes and elicits intracellular signaling. INSRR is predominantly expressed in organs that come in contact with mildly alkaline media, and is postulated to be an alkali sensor to manage metabolic bicarbonate excess in kidney (3). Mutations in INSRR have been linked to severe insulin resistance (type A and Rabson-Mendenhall syndrome), type II diabetes mellitus, and leprechaunism (Donohue syndrome) (4, 5). Human INSRR shares 89% identity with mouse and rat INSRR.

References
  1. Nef, S. et al. (2003) Nature 426:291.
  2. Lee, J. & Pilch, P. F. (1994) Am J Physiol Cell Physiol. 266:C319.
  3. Deyev, I. E. et al. (2011) Cell Metab. 13:679.
  4. Kobayashi, M. et al. (1978). Proc Natl Acad Sci U.S.A. 75:3469.
  5. Musso, C. et al. (2004). Medicine 83:209.
Long Name
Insulin Receptor-related Receptor
Entrez Gene IDs
3645 (Human); 23920 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; INSRR; insulin receptor-related protein; insulin receptor-related receptor; IRR; IRRIR-related receptor

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