Recombinant Human Kallikrein 5 Protein, CF

Catalog # Availability Size / Price Qty
Kallikrein 5 Protein Enzyme Activity
1 Image
Product Details
Citations (17)
Supplemental Products
Reviews (1)

Recombinant Human Kallikrein 5 Protein, CF Summary

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011). The specific activity is >200 pmol/min/µg, as measured under the described conditions. 
Mouse myeloma cell line, NS0-derived human Kallikrein 5 protein
Ile67-Ser293, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Structure / Form
Mature form
Predicted Molecular Mass
31 kDa
40 kDa, reducing conditions

Product Datasheets

Data Image

Enzyme Activity Kallikrein 5 Protein Enzyme Activity View Larger

Recombinant Human Kallikrein 5 (Catalog # 1108-SE) is measured byits ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Kallikrein 5

Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (2). The deduced amino acid sequence of human KLK5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). KLK5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). KLK5 overexpression is an indicator of poor prognosis in ovarian cancer (4). hK5 is a candidate physiological activator of hK7 in the stratum corneum (5). Indeed, rhK5 can be used to activate rhK7 (R&D Systems, Catalog # 2624‑SE) by incubating rhK5 with recombinant human (rh) K7 (both at 0.1 mg/mL) in 25 mM MES, 0.15 M NaCl, pH 5.5, at 37 °C overnight.

rhK5 enzymatic activity can be inhibited by rhSerpinA1, A5, F2, and G1 (R&D Systems, Catalog # 1268‑PI, 1266‑PI, 1470-PI, 2488‑PI).

  1. Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
  2. Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
  3. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  4. Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
  5. Kim, H. et al. (2001) Br. J. Cancer 84:643.
Entrez Gene IDs
25818 (Human); 68668 (Mouse); 690603 (Rat)
Alternate Names
EC 3.4.21; EC; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme

Citations for Recombinant Human Kallikrein 5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Kallikrein-related peptidase 5 contributes to H3N2 influenza virus infection in human lungs
    Authors: M Magnen, F Gueugnon, A Guillon, T Baranek, VC Thibault, A Petit-Cour, SJ de Veer, J Harris, AA Humbles, M Si-Tahar, Y Courty
    J. Virol., 2017;0(0):.
    Species: Virus
    Sample Types: Protein
    Applications: EnzAct
  2. KLK5 induces shedding of DPP4 from circulatory Th17 cells in type 2 diabetes
    Authors: T Nargis, K Kumar, AR Ghosh, A Sharma, D Rudra, D Sen, S Chakrabart, S Mukhopadhy, D Ganguly, P Chakrabart
    Mol Metab, 2017;6(11):1529-1539.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A peptide-based approach to evaluate the adaptability of influenza A virus to humans based on its hemagglutinin proteolytic cleavage site
    Authors: MR Straus, GR Whittaker
    PLoS ONE, 2017;12(3):e0174827.
    Species: Influenza A Virus
    Sample Types: Peptide
    Applications: EnzAct
  4. Protease-inhibitor interaction predictions: Lessons on the complexity of protein-protein interactions
    Authors: N Fortelny, GS Butler, CM Overall, P Pavlidis
    Mol. Cell Proteomics, 2017;0(0):.
    Applications: Bioassay
  5. Inhibition of Human Kallikrein 5 Protease by Triterpenoids from Natural Sources
    Authors: Y Matsubara, T Matsumoto, J Koseki, A Kaneko, S Aiba, K Yamasaki
    Molecules, 2017;22(11):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Defects in Stratum Corneum Desquamation Are the Predominant Effect of Impaired ABCA12 Function in a Novel Mouse Model of Harlequin Ichthyosis
    PLoS ONE, 2016;11(8):e0161465.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Anti-inflammatory and immunomodulatory effects of Aquaphilus dolomiae extract on in vitro models
    Authors: Nathalie Castex-Riz
    Clin Cosmet Investig Dermatol, 2016;9(0):421-434.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation.
    Authors: Yamamoto-Tanaka M, Motoyama A, Miyai M, Matsunaga Y, Matsuda J, Tsuboi R, Hibino T
    J Biol Chem, 2014;289(29):20026-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Cross-linking of SPINK6 by transglutaminases protects from epidermal proteases.
    Authors: Fischer J, Koblyakova Y, Latendorf T, Wu Z, Meyer-Hoffert U
    J Invest Dermatol, 2013;133(5):1170-7.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  10. Proteolytic activation cascade of the Netherton syndrome-defective protein, LEKTI, in the epidermis: implications for skin homeostasis.
    Authors: Fortugno P, Bresciani A, Paolini C, Pazzagli C, El Hachem M, D'Alessio M, Zambruno G
    J Invest Dermatol, 2011;131(11):2223-32.
  11. Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome.
    Authors: Sales KU, Masedunskas A, Bey AL
    Nat. Genet., 2010;42(8):676-83.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  12. Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid.
    Authors: Morizane S, Yamasaki K, Kabigting FD, Gallo RL
    J. Invest. Dermatol., 2010;130(5):1297-306.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  13. Isolation of SPINK6 in human skin: selective inhibitor of kallikrein-related peptidases.
    Authors: Meyer-Hoffert U, Wu Z, Kantyka T, Fischer J, Latendorf T, Hansmann B, Bartels J, He Y, Glaser R, Schroder JM
    J. Biol. Chem., 2010;285(42):32174-81.
    Species: Human
    Sample Types: Peptide
    Applications: Enzyme Assay
  14. Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases.
    Authors: Sexton DJ, Chen T, Martik D, Kuzmic P, Kuang G, Chen J, Nixon AE, Zuraw BL, Forteza RM, Abraham WM, Wood CR
    Biochem. J., 2009;422(2):383-92.
    Species: Human
    Sample Types: Antibody
    Applications: Surface Plasmon Resonance
  15. Activation of hepatocyte growth factor activator zymogen (pro-HGFA) by human kallikrein 1-related peptidases.
    Authors: Mukai S, Fukushima T, Naka D, Tanaka H, Osada Y, Kataoka H
    FEBS J., 2008;275(5):1003-17.
    Species: Human
    Sample Types: Protein
    Applications: EnzAct
  16. Increased serine protease activity and cathelicidin promotes skin inflammation in rosacea.
    Authors: Yamasaki K, Di Nardo A, Bardan A, Murakami M, Ohtake T, Coda A, Dorschner RA, Bonnart C, Descargues P, Hovnanian A, Morhenn VB, Gallo RL
    Nat. Med., 2007;13(8):975-80.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6.
    Authors: Kantyka T, Fischer J, Wu Z, Declercq W, Reiss K, Schroder J, Meyer-Hoffert U
    Peptides, 0;32(6):1187-92.
    Applications: Bioassay


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Kallikrein 5 Protein, CF

Average Rating: 4 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Recombinant Human Kallikrein 5 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Recombinant Human Kallikrein 5 Protein, CF
By Anonymous on 02/09/2017
Application: In vitro bioactivity in cell culture
Reason for Rating: we use it as standard in elisa