Recombinant Human LAIR1 His-tag Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI2664-050
Recombinant Human LAIR1 His-tag Avi-tag Protein, CF Binding Activity
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Recombinant Human LAIR1 His-tag Avi-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Bovine Collagen is coated at 10 µg/mL, 100 μL/well, Biotinylated Recombinant Human LAIR1 His-tag Avi-tag (Catalog # AVI2664) binds with an ED50 of 0.6-4.8.
Source
Human embryonic kidney cell, HEK293-derived human LAIR1 protein
Human LAIR1
(Gln22-His163)
Accession # NP_002278.2
HHHHHH Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln22 inferred from enyzmatic pyroglutamate treatment revealing Glu23
Structure / Form
Biotinylated via Avi-tag
Predicted Molecular Mass
18 kDa
SDS-PAGE
25-38 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI2664

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Bovine Collagen is coated at 10 μg/mL, 100 μL/well, Biotinylated Recombinant Human LAIR1 His-tag Avi-tag (Catalog # AVI2664) binds with an ED50 of 0.6-4.8 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human LAIR1 His-tag Avi-tag (Catalog # AVI2664) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 25-38 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAIR1

LAIR1 (leukocyte-associated Ig-like receptor-1, designated CD305) is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily (1-4). LAIR1 is a collagen-binding protein that is expressed in a differentiation- and activation-dependent manner on most immune cells, including T, B, NK and dendritic cells (DC), monocytes, CD34+ hematopoietic progenitors, most thymocytes, and selected granulocyte populations (2-7). Mature human LAIR1 is a 266 amino acid (aa) type I transmembrane protein that includes a 144 aa extracellular domain (ECD) with one collagen-binding C2-type Ig-like domain, and a 101 aa cytoplasmic domain with two ITIM motifs (2, 3, 8, 9). Of four potential human LAIR1 splice variants, LAIR1b has a 17 aa deletion within the ECD, but outside the Ig domain. LAIR1c differs from LAIR1b by one aa. LAIR1d has a 78 aa cytoplasmic truncation and lacks ITIM motifs. Human LAIR1 ECD shares <45% aa sequence identity with mouse, rat, bovine or canine LAIR1 ECD, but all are functional orthologs. Humans, but not rodents, also express the 152 aa secreted protein LAIR2, which shares 83% aa sequence identity with the LAIR1 ECD up to aa 140 and can block LAIR1 collagen binding (1, 2). A soluble form of LAIR1 found in plasma and urine also binds collagen (10).  Adhesion of LAIR1 to collagens in the extracellular matrix, transmembrane collagens expressed by tumor cells, or antibody-mediated crosslinking of LAIR1, inhibits signals relayed by ITAM-bearing receptors and some cytokine-mediated signals (6-8, 13).  Processes that are inhibited include B and T cell receptor-mediated activation, NK and T cell‑mediated cytotoxicity, and basophil degranulation (1-4, 8). LAIR1 is reduced or absent on chronic lymphocytic leukemia (CLL) B cells, and some B and DC cells in systemic lupus erythematosus (SLE). Its under‑expression potentially enhances CLL proliferation and SLE immune responses (7, 11, 12).

References
  1. Meyaard, L. (2008) J. Leukoc. Biol. 83:799.
  2. Meyaard, L. et al. (1997) Immunity 7:283.
  3. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535.
  4. Ouyang, W. et al. (2003) Biochem. Biophys. Res. Commun. 310:1236.
  5. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828.
  6. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.
  7. Bonaccorsi, I. et al. (2010) PLoS ONE 5:e15080.
  8. Tang, X. et al. (2009) J. Immunol. 182:5446.
  9. Brondijk, T.H.C. et al. (2010) Blood 115:1364.
  10. Olde Nordkamp, M.J. et al. (2011) Arthritis Rheum. 63:3749.
  11. Poggi, A. et al. (2008) Leukemia 22:980.
  12. Colombo, B.M. et al. (2012) PLoS ONE 7:e31903.
  13. Rygiel, T.P. et al. (2011) Mol. Immunol. 49(1-2):402.
Long Name
Leukocyte-associated Ig-like Receptor 1
Entrez Gene IDs
3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)
Alternate Names
CD305 antigen; CD305; CD305leukocyte-associated Ig-like receptor 1; HLAIR1; LAIR1; LAIR-1; leukocyte-associated immunoglobulin-like receptor 1

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