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Recombinant Human LC3A Rhodamine 110 Protein, CF

Catalog #: UL-455 Datasheet / COA / SDS

Discontinued Product

UL-455 has been discontinued.
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Recombinant Human LC3A Rhodamine 110 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human MAP1LC3A Rhodamine is ideal for use in assays requiring fluorescent detection. Optimal fluorescence at pH 8.0 is monitored with an excitation wavelength of 485 nm and an emission wavelength of 535 nm. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human MAP1LC3A Rhodamine concentration of 0.1-1 μM.
Source
E. coli-derived human LC3A protein
Accession #
Q9H492
Predicted Molecular Mass
14 kDa

Product Datasheets

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UL-455

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-455

Formulation

0.62 mg/ml (44 µM) in 50 mM MES, pH 6.0, 100 mM NaCl, 20% glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LC3A

Microtubule-associated proteins 1A/1B light chain 3A (also known as MAP1LC3A) is an Ubiquitin-like modifier of the ATG8 family and is involved in the formation of the autophagosomes. This protein interacts directly with Sequestosome-1 and is recruited to inclusion bodies containing polyubiquitinated protein aggregates where it plays a role in inclusion body degradation by autophagy. MAP1LC3A-Rhodamine110 (Rh110) is a fluorogenic substrate for the autophagy hydrolase ATG4B. This substrate is useful for studying ATG4B when detection sensitivity or continuous monitoring of activity is essential.

References
  1. Borodovsky A., et al. (2002) Chem. Biol. 9:1149-1159
  2. Hemelaar J., et al. (2003) J Biol. Chem. 278:51841-51850
  3. Kessler B.M. (2006) Exp. Rev. Proteomics. 3:213-221
  4. Kumanomidou T., et al. (2006) J. Mol. Biol. 355:612-618
  5. Love K.R, et al. (2007) Nat. Chem. Biol. 3:697-705
  6. Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51
Long Name
Microtubule-associated Protein 1 Light Chain 3 alpha
Entrez Gene IDs
84557 (Human); 66734 (Mouse); 362245 (Rat); 852200 (Yeast)
Alternate Names
Apg8; APG8a; Apg8p3; ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; MAP1LC3A; Microtubule-associated protein 1 light chain 3 alpha; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A; MLP3A

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