Recombinant Human LIF Protein

Formulations:
Catalog # Availability Size / Price Qty
7734-LF-025
7734-LF-100
7734-LF-500
Product Details
Citations (13)
FAQs
Reviews (2)

Recombinant Human LIF Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.12 ng/mL. Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 for this effect is approximately 0.5 ng/mL.
Source
E. coli-derived human LIF protein
Pro24-Phe202
Accession #
N-terminal Sequence
Analysis
Pro24
Predicted Molecular Mass
19.6 kDa
SDS-PAGE
19 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7734-LF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7734-LF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIF

LIF (leukemia inhibitory factor) is a widely expressed, highly and variably glycosylated, 32‑62 kDa, monomeric, pleiotropic cytokine in the IL‑6 family of helical cytokines (1‑4). The first exon encoding the signal sequence is alternately spliced, resulting in LIF-D, LIF-M, and LIF‑T mRNAs that produce secreted, extracellular matrix‑associated, and intracellular forms, respectively (5). LIF-D and LIF-M mRNAs produce identical 180 amino acid (aa) mature sequences (5). Mature human LIF (180 aa) shares 78%, 82%, 91%, 88 and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. The LIF receptor is a heterodimer of a type I transmembrane ligand‑binding subunit, LIFR (gp190), and the type I transmembrane signal transducing subunit, gp130, signaling especially through STAT3 and JAK kinases (3, 4, 6). Gp130 and members of the LIFR family also mediate the biological effects of Oncostatin M, Cardiotrophin‑1, Galectin‑10, CNTF, IL‑6,
IL‑11, and IL‑27 (3, 6). A soluble LIFR has been reported in the mouse (7). Depending on the cells and their context, LIF either opposes or favors differentiation (3, 8). LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells (3, 6, 8). However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation (3, 6). LIF is produced by CD4+ T cells in response to activation, and is required by the thymic epithelium to support T cell maturation (3, 4). LIF expression is up‑regulated by neuronal injury, and promotes motor neuron survival and oligodendrocyte myelination (3, 4, 9). LIF is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone (10). LIF can function as an autocrine growth factor in some pancreatic cancers, but induces differentiation in the myeloid leukemic cell line M1 (2, 11). Tumor LIF can also induce formation of immunosuppressive tumor‑associated macrophages (12). LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells (3, 4). It promotes regulatory T cell and inhibits Th17 cell differentiation, thus promoting tolerance, down‑regulating inflammation, and contributing to immune tolerance during pregnancy and in the nervous system (3, 4, 6, 8).

References
  1. Gough, N.M. et al. (1988) Proc. Natl. Acad. Sci. USA 85:2623.
  2. Moreau, J.F. et al. (1988) Nature 336:690.
  3. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51.
  4. Metcalfe, S.M. (2011) Genes Immun. 12:157.
  5. Voyle, R.B. et al. (1999) Exp. Cell Res. 249:199.
  6. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
  7. Tomida, M. et al. (1993) FEBS lett. 334:193.
  8. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  9. Slaets, H. et al. (2010) Trends Mol. Med. 16:493.
  10. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145.
  11. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977.
  12. Duluc, D. et al. (2007) Blood 110:4319.
Long Name
Leukemia Inhibitory Factor
Entrez Gene IDs
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names
CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI

Citations for Recombinant Human LIF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. The transcription factor Vezf1 represses the expression of the antiangiogenic factor d2 in endothelial cells
    Authors: L Alabdi, M He, Q Yang, AB Norvil, H Gowher
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. STAT3 signaling stimulates miR-21 expression in bovine cumulus cells during in vitro oocyte maturation
    Authors: A Tscherner, AC Brown, L Stalker, J Kao, I Dufort, MA Sirard, J LaMarre
    Sci Rep, 2018;8(1):11527.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  3. LIFR increases the release of sEng via the up-regulation of MMP14 expression in PE
    Authors: H Li, J Yao, X Chang, J Wu, T Duan, K Wang
    Reproduction, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. miR-124 promotes proliferation and differentiation of neuronal stem cells through inactivating Notch pathway
    Authors: S Jiao, Y Liu, Y Yao, J Teng
    Cell Biosci, 2017;7(0):68.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke
    Authors: H Zhang, F Sun, J Wang, L Xie, C Yang, M Pan, B Shao, GY Yang, SH Yang, Q ZhuGe, K Jin
    Aging Dis, 2017;8(2):203-214.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Increased susceptibility to a? toxicity in neuronal cultures derived from familial Azheimer's disease (PSEN1-A246E) induced pluripotent stem cells
    Authors: Enrique Armijo
    Neurosci. Lett, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow
    Nat Cell Biol, 2016;18(10):1078-1089.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells.
    Authors: Geng, Zhe, Li, Ping, Tan, Li, Song, Houyan
    Stem Cells Int, 2015;2015(0):657325.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. A Novel Small-Molecule Inhibitor Targeting the IL-6 Receptor beta Subunit, Glycoprotein 130.
    Authors: Hong S, Choi J, Lee S, Park Y, Park K, Lee J, Kim J, Gajulapati V, Goo J, Singh S, Lee K, Kim Y, Im S, Ahn S, Rose-John S, Heo T, Choi Y
    J Immunol, 2015;195(1):237-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M.
    Authors: Kwofie K, Scott M, Rodrigues R, Guerette J, Radford K, Nair P, Richards C
    Respir Res, 2015;16(0):14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Cancer-associated adipose tissue promotes breast cancer progression by paracrine oncostatin M and Jak/STAT3 signaling.
    Authors: Lapeire L, Hendrix A, Lambein K, Van Bockstal M, Braems G, Van Den Broecke R, Limame R, Mestdagh P, Vandesompele J, Vanhove C, Maynard D, Lehuede C, Muller C, Valet P, Gespach C, Bracke M, Cocquyt V, Denys H, De Wever O
    Cancer Res, 2014;74(23):6806-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. The amino acid exchange R28E in ciliary neurotrophic factor (CNTF) abrogates interleukin-6 receptor-dependent but retains CNTF receptor-dependent signaling via glycoprotein 130 (gp130)/leukemia inhibitory factor receptor (LIFR).
    Authors: Wagener E, Aurich M, Aparicio-Siegmund S, Floss D, Garbers C, Breusing K, Rabe B, Schwanbeck R, Grotzinger J, Rose-John S, Scheller J
    J Biol Chem, 2014;289(26):18442-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Focal transplantation of human iPSC-derived glial-rich neural progenitors improves lifespan of ALS mice.
    Authors: Kondo T, Funayama M, Tsukita K, Hotta A, Yasuda A, Nori S, Kaneko S, Nakamura M, Takahashi R, Okano H, Yamanaka S, Inoue H
    Stem Cell Reports, 2014;3(2):242-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human LIF Protein

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Recombinant Human LIF Protein
By Anonymous on 04/09/2017
Application: Cell migration/motility

Growth of MA-11 spheroids in serum-free condition in the absence (A) or presence (B) of Recombinant Human LIF (#7734-LF) after 72 hours. Medium: RPMI + 2% B-27 supplement + 1% L-glutamine + 1% Pen/Strep.


Recombinant Human LIF Protein
By Anonymous on 08/03/2016
Application: Stem/Immune cell maintenance or differentiation