Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

R&D Systems | Catalog # 8429-T4

(HEK293-expressed)
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein (8429-T4)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q8N423.4

Structure / Form

Monomer

Applications

Bioactivity
View all LILRB2/CD85d/ILT4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LILRB2/CD85d/ILT4 protein
Gln22-His458, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Thr23

Predicted Molecular Mass

48 kDa

SDS-PAGE

62-72 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LILRB2/CD85d/ILT4 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 of 25-150 ng/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 8429-T4 in the following applications:

Scientific Data Images for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein Bioactivity

Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein Bioactivity

When Recombinant Human LILRB2/CD85d/ILT4 (Catalog # 8429-T4) is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like 7 Protein (Catalog # 914-AN) binds with a typical ED50 of 25-150 ng/mL.
Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein SDS-PAGE

Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein SDS-PAGE

1 μg/lane of Recombinant Human LILRB2/CD85d/ILT4 (Catalog # 8429-T4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 69.9 and 66.8 kDa, respectively.

Formulation, Preparation, and Storage

8429-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

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Background: LILRB2/CD85d/ILT4

The immunoglobulin-like transcript (ILT) molecules comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR) (1, 2). ILT4, also known as CD85d, LIR-2, and LILRB2, is closely related to mouse PIR-B and is primarily expressed on monocytes, dendritic cells (DC), and neutrophils (3, 4). Mature human ILT4 consists of a 440 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 116 aa cytoplasmic domain with 3 inhibitory immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (5). The ECD of human ILT4 shares 76% aa identity with chimpanzee ILT4 and 74%, 81%, 33%, 52%, 77%, 61%, and 64% aa identity with human ILT1, 2, 3, 5, 6, 7, and 8, respectively. ILT4 binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (5-9). It competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1 (7). Ligation of ILT4 induces tyrosine phosphorylation within its cytoplasmic ITIMs, association with SHP-1, and inhibition of stimulatory signaling (3, 6). ILT4 activation promotes the development of tolerogenic dendritic cells and the subsequent induction of regulatory T cells and CD4+ T cell anergy (10-12). Ligation of ILT4 on neutrophils further attenuates immune responses by inhibiting neutrophil phagocytic activity and production of reactive oxygen species (4). ILT4 also binds to multimeric Angiopoietin-like 2, and its ligation supports the expansion of hematopoietic stem cells from cord blood (13). In the brain, ILT4 and mouse PIR-B function as receptors for oligomeric Abeta (1-42) peptide (14).

References

  1. Suciu-Foca, N. et al. (2005) Int. Immunopharmacol. 5:7.
  2. Hofmeister, V. and E.H. Weiss (2003) Semin. Canc. Biol. 13:317.
  3. Fanger, N.A. et al. (1998) Eur. J. Immunol. 28:3423.
  4. Baudhuin, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:17957.
  5. Borges, L. et al. (1997) J. Immunol. 159:5192.
  6. Colonna, M. et al. (1998) J. Immunol. 160:3096.
  7. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
  8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
  9. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  10. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  11. Ristich, V. et al. (2005) Eur. J. Immunol. 35:1133.
  12. Gregori, S. et al. (2010) Blood 116:935.
  13. Deng, M. et al. (2014) Blood 124:924.
  14. Kim, T. et al. (2013) Science 341:1399.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5

Alternate Names

CD85d, ILT4, LIR2, MIR10

Entrez Gene IDs

10288 (Human)

Gene Symbol

LILRB2

UniProt

Q8N423.4

Additional LILRB2/CD85d/ILT4 Products

Product Documents for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

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Product Specific Notices for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

For research use only

Citations for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Customer Reviews for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF (2)

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  • Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 09/01/2021
    Immobilize HLA-G and flow ILT4. Reverse orientation also shows binding
    Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF 8429-T4
  • Name: Anonymous
    Verified Customer | Posted 04/28/2020
    Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF 8429-T4

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