Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

(HEK293-expressed)
Catalog # Availability Size / Price Qty
8429-T4-050

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Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein Bioactivity
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Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB2/CD85d/ILT4 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 of 25-150 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human LILRB2/CD85d/ILT4 protein
Gln22-His458, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Thr23
Structure / Form
Monomer
Predicted Molecular Mass
48 kDa
SDS-PAGE
62-72 kDa, reducing conditions

Product Datasheets

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8429-T4

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8429-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein Bioactivity View Larger

When Recombinant Human LILRB2/CD85d/ILT4 (Catalog # 8429-T4) is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like 7 Protein (Catalog # 914-AN) binds with a typical ED50 of 25-150 ng/mL.

SDS-PAGE Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human LILRB2/CD85d/ILT4 (Catalog # 8429-T4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 69.9 and 66.8 kDa, respectively.

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Background: LILRB2/CD85d/ILT4

The immunoglobulin-like transcript (ILT) molecules comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR) (1, 2). ILT4, also known as CD85d, LIR-2, and LILRB2, is closely related to mouse PIR-B and is primarily expressed on monocytes, dendritic cells (DC), and neutrophils (3, 4). Mature human ILT4 consists of a 440 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 116 aa cytoplasmic domain with 3 inhibitory immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (5). The ECD of human ILT4 shares 76% aa identity with chimpanzee ILT4 and 74%, 81%, 33%, 52%, 77%, 61%, and 64% aa identity with human ILT1, 2, 3, 5, 6, 7, and 8, respectively. ILT4 binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (5-9). It competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1 (7). Ligation of ILT4 induces tyrosine phosphorylation within its cytoplasmic ITIMs, association with SHP-1, and inhibition of stimulatory signaling (3, 6). ILT4 activation promotes the development of tolerogenic dendritic cells and the subsequent induction of regulatory T cells and CD4+ T cell anergy (10-12). Ligation of ILT4 on neutrophils further attenuates immune responses by inhibiting neutrophil phagocytic activity and production of reactive oxygen species (4). ILT4 also binds to multimeric Angiopoietin-like 2, and its ligation supports the expansion of hematopoietic stem cells from cord blood (13). In the brain, ILT4 and mouse PIR-B function as receptors for oligomeric Abeta (1-42) peptide (14).

References
  1. Suciu-Foca, N. et al. (2005) Int. Immunopharmacol. 5:7.
  2. Hofmeister, V. and E.H. Weiss (2003) Semin. Canc. Biol. 13:317.
  3. Fanger, N.A. et al. (1998) Eur. J. Immunol. 28:3423.
  4. Baudhuin, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:17957.
  5. Borges, L. et al. (1997) J. Immunol. 159:5192.
  6. Colonna, M. et al. (1998) J. Immunol. 160:3096.
  7. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
  8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
  9. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  10. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  11. Ristich, V. et al. (2005) Eur. J. Immunol. 35:1133.
  12. Gregori, S. et al. (2010) Blood 116:935.
  13. Deng, M. et al. (2014) Blood 124:924.
  14. Kim, T. et al. (2013) Science 341:1399.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5
Entrez Gene IDs
10288 (Human)
Alternate Names
CD85 antigen-like family member D; CD85d antigen; CD85d; Ig-like transcript 4; ILT4; ILT-4; ILT4CD85d; Immunoglobulin-like transcript 4; Leukocyte immunoglobulin-like receptor 2; leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 2; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB2; LIR2; LIR2CD85D; LIR-2subfamily A (with TM domain), member 6; member 2; MIR10; MIR10LILRA6; Monocyte/macrophage immunoglobulin-like receptor 10

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Reviews for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF
By Anonymous on 09/01/2021
Application: Binding assay/Protein-protein interaction
Reason for Rating: HLA-G reagent shows specific binding to ILT4. Binding is weak so could not get KD

Immobilize HLA-G and flow ILT4. Reverse orientation also shows binding


By Anonymous on 04/28/2020

Reason for Rating: Great reagent for capturing anti-RB2 antibody