Recombinant Human LILRB3/CD85a/ILT5 Protein, CF

R&D Systems | Catalog # 9159-T5

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human LILRB3/CD85a/ILT5 Protein (9159-T5)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

AAB68668

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LILRB3/CD85a/ILT5 protein
Gly24-Glu443, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

47 kDa

SDS-PAGE

60-69 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LILRB3/CD85a/ILT5 is coated at 2 μg/mL, Recombinant Human Angiopoietin-like 7 (Catalog # 914-AN) binds with an ED50 = 50-300 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 9159-T5 in the following applications:

Scientific Data Images for Recombinant Human LILRB3/CD85a/ILT5 Protein, CF

Recombinant Human LILRB3/CD85a/ILT5 Protein Bioactivity

Recombinant Human LILRB3/CD85a/ILT5 Protein Bioactivity

When Recombinant Human LILRB3/CD85a/ILT5 (Catalog # 9159-T5) is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like 7 (Catalog # 914-AN) binds with an ED50 of 50-300 ng/mL.

Formulation, Preparation, and Storage

9159-T5
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB3/CD85a/ILT5

Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. Subfamily A members are activating receptors that lack ITIMs and signal through association with FcR gamma (1, 2). Mature LILRB3 is a highly polymorphic 85-95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3). Alternative splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human LILRB3 share 55% aa sequence identity within common regions of their ECDs. LILRB3 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors (4-6). Triggering of LILRB3 inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5, 7). On osteoclast precursors, LILRB3 ligation inhibits RANK L/TRANCE or M-CSF induced differentiation (8). LILRB3 can also bind to ligands exposed on necrotic tumor cells (9). Both PIR-B and LILRB3 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (3). R&D Systems in-house testing indicates that LILRB3 binds to
Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (10). In the mouse CNS, PIR-B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (11). Upon binding to MAG, PIR-B associates with TrkB and NGF R/p75 in cerebellar granule neurons (12).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
  3. Nakayama, M. et al. (2007) J. Immunol. 178:4250.
  4. Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1174.
  5. Sloane, D.E. et al. (2004) Blood 104:2832.
  6. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  7. Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
  8. Mori, Y. et al. (2008) J. Immunol. 181:4742.
  9. Jones, D.C. et al. (2016) Oncotarget PMID 26769854.
  10. Zheng, J. et al. (2012) Nature 485:656.
  11. Atwal, J.K. et al. (2008) Science 322:967.
  12. Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.

Alternate Names

CD85a, ILT5, LIR3

Entrez Gene IDs

11025 (Human)

Gene Symbol

LILRB3

UniProt

AAB68668

Additional LILRB3/CD85a/ILT5 Products

Product Documents for Recombinant Human LILRB3/CD85a/ILT5 Protein, CF

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Product Specific Notices for Recombinant Human LILRB3/CD85a/ILT5 Protein, CF

For research use only

Citations for Recombinant Human LILRB3/CD85a/ILT5 Protein, CF

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  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/06/2020
    Performed binding specificity experiments for the lead molecule (xLILRA6) on its ligand family panels (RA1, RA2, RA5, RA6, RB1, RB2, RB3, RB4, RB5)
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