Recombinant Human LILRB3/CD85a/ILT5 Protein, CF Summary
Gly24-Glu443, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human LILRB3/CD85a/ILT5 (Catalog # 9159-T5) is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like 7 (Catalog # 914-AN) binds with an ED50 of 50-300 ng/mL.
Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. Subfamily A members are activating receptors that lack ITIMs and signal through association with FcR gamma (1, 2). Mature LILRB3 is a highly polymorphic 85-95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3). Alternative splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human LILRB3 share 55% aa sequence identity within common regions of their ECDs. LILRB3 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors (4-6). Triggering of LILRB3 inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5, 7). On osteoclast precursors, LILRB3 ligation inhibits RANK L/TRANCE or M-CSF induced differentiation (8). LILRB3 can also bind to ligands exposed on necrotic tumor cells (9). Both PIR-B and LILRB3 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (3). R&D Systems in-house testing indicates that LILRB3 binds to
Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (10). In the mouse CNS, PIR-B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (11). Upon binding to MAG, PIR-B associates with TrkB and NGF R/p75 in cerebellar granule neurons (12).
- Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
- Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
- Nakayama, M. et al. (2007) J. Immunol. 178:4250.
- Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1174.
- Sloane, D.E. et al. (2004) Blood 104:2832.
- Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
- Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
- Mori, Y. et al. (2008) J. Immunol. 181:4742.
- Jones, D.C. et al. (2016) Oncotarget PMID 26769854.
- Zheng, J. et al. (2012) Nature 485:656.
- Atwal, J.K. et al. (2008) Science 322:967.
- Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.
Citation for Recombinant Human LILRB3/CD85a/ILT5 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.
Authors: Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C
Sample Types: Recombinant Protein
Applications: Biolayer Interferometry (BLI)
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