Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein, CF

Biotinylated His-tag
Catalog # Availability Size / Price Qty
AVI8884-025
Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein, CF SDS-PAGE
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Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Lymphotoxin beta R Fc Chimera (Catalog # 629-LR) is immobilized at 25 ng/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human Lymphotoxin protein
Human Lymphotoxin alpha
(Leu35-Leu205)
Accession # P01374
GGGGS

Human Lymphotoxin beta
(Gln49-Gly244)
Accession # Q06643-1 

GGGGS 

Human Lymphotoxin beta
(Gln49-Gly244)
Accession # Q06643-1 

HHHHHH, Avi-tag 
N-terminus
N-terminal Sequence
Analysis
Leu35
Structure / Form
GS-linked heterotrimer, biotinylated via Avi-tag
Predicted Molecular Mass
63 kDa
SDS-PAGE
69-80 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI8884

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 69-80 kDa.

Binding Activity Binding Activity View Larger

When Recombinant Human Lymphotoxin  beta R/TNFRSF3 Fc Chimera (Catalog # 629-LR) is immobilized at 25 ng/mL, 100 μg/well, Biotinylated Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) binds with an ED50 of 0.75‑4.5 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin

Lymphotoxin alpha (LT-alpha ), and Lymphotoxin beta (LT-beta ) are pro-inflammatory TNF superfamily ligands that play important roles in immune system development (1, 2). The 25 kDa mature human LT-alpha is a secreted protein that shares 75% amino acid (aa) sequence identity with mouse and rat LT-alpha (3, 4). The 33 kDa mature human LT-beta is a type II transmembrane protein that shares 73% aa sequence identity with mouse and rat LT-beta within common regions of their extracellular domains (5). Relative to the human protein, mouse and rat LT-beta have a 66 aa or 65 aa insertion within the ECD, respectively. LT-alpha can be secreted as a homotrimer that binds and activates TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, HVEM/TNFRSF14, and Troy/TNFRSF19 (6-8). LT-alpha is required for development of the spleen, lymph nodes, and Peyer’s patches (9). It also regulates T cell homing to the gut and IgA induction (10). In addition, LT-alpha can form membrane-associated heterotrimers with two copies of LT-beta on the surface of B, T, LTi, and ILC3 cells (2, 5, 11). The Lymphotoxin alpha 1/ beta 2 heterotrimer binds and activates the Lymphotoxin beta R/TNFRSF3 (LT beta R) which is expressed on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV) (2, 12, 13). LT beta R also serves as a receptor for LIGHT/TNFSF14 (14). LT-alpha 1/ beta 2 promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by ILCs (10, 15-17). It can be shed by ADAM17 or MMP-8 mediated cleavage, and the released heterotrimer circulates in the serum and is elevated in synovial fluid of rheumatoid arthritis patients (18).

References
  1. Lu, T.T. and J.L. Browning (2014) Front. Immunol. 5:47.
  2. Upadhyay, V. and Y.-X. Fu (2014) Cytokine Growth Factor Rev. 25:227.
  3. Gray, P.W. et al. (1984) Nature 312:721.
  4. Nedwin, G.E. et al. (1985) J. Cell Biochem. 29:171.
  5. Browning, J.L. et al. (1993) Cell 72:847.
  6. Schoenfeld, H.J. et al. (1991) J. Biol. Chem. 266:3863.
  7. Mauri, D.N. et al. (1998) Immunity 8:21.
  8. Hashimoto, T. et al. (2008) Cell Cycle 7:106.
  9. De Togni, P. et al. (1994) Science 264:703.
  10. Kruglov, A.A. et al. (2013) Science 342:1243.
  11. Androlewicz, M.J. et al. (1992) J. Biol. Chem. 267:2542.
  12. Sudhamsu, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:19896.
  13. Crowe, P.D. et al. (1994) Science 264:707.
  14. Eldredge, J. et al. (2006) Biochemistry 45:10117.
  15. Futterer, A. et al. (1998) Immunity 9:59.
  16. Koni, P.A. et al. (1997) Immunity 6:491.
  17. Ota, N. et al. (2011) Nat. Immunol. 12:941.
  18. Young, J. et al. (2010) Cytokine 51:78.
Entrez Gene IDs
4049 (Human); 16992 (Mouse)
Alternate Names
LT; Lymphotoxin alpha; Lymphotoxin; tnfb; TNFSF1; TNLG1E

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