Recombinant Human MCAM/CD146 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9709-MA-050
Recombinant Human MCAM/CD146 Fc Chimera Protein Bioactivity
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Citations (2)
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Recombinant Human MCAM/CD146 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human MCAM/CD146 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, it binds Recombinant Human Galectin-3 (Catalog # 8259-GA). The concentration of Recombinant Human Galectin-3 that produces 50% of the optimal binding response is 0.25‑1.25 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human MCAM/CD146 protein
Human MCAM/CD146
(Val24-Gly559)
Accession # AAA20922
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
87 kDa
SDS-PAGE
108-124 kDa, reducing condtions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9709-MA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human MCAM/CD146 Fc Chimera Protein Bioactivity View Larger

When Recombinant Human MCAM/CD146 Fc Chimera is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Galectin-3 (Catalog # 8259-GA) binds with an ED50 of 0.25‑1.25 ug/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MCAM/CD146

Melanoma cell adhesion molecule (MCAM), also known as MUC18 or CD146, is a putative adhesion molecule that belongs to the immunoglobulin superfamily (IgSF) (1). MCAM is an approximately 113 kDa type I transmembrane glycoprotein that contains a 536 amino acid (aa) extracellular domain (ECD), a 24 aa transmembrane domain, and a 63 aa cytoplasmic domain. Two MCAM splice variants have been observed, which vary in the length of their cytoplasmic tail (2). The ECD of human MCAM contains 2 IgV and 3 IgC2 domains and shares 74% and 73% identity with mouse and rat, respectively. MCAM was originally described as a marker of malignant potential in melanoma and was reported to promote both invasion and metastasis (3). Since then, expression has been detected in endothelial cells throughout the body and MCAM has been shown to be involved multiple cellular events including adhesion, migration, proliferation and differentiation (4, 5). Additionally, MCAM has been implicated in recruitment of activated T cells to inflammatory sites and is up-regulated in various inflammatory diseases (5, 6). Inhibiting MCAM signaling has been suggested as a potential therapy for diverse diseases including inflammatory bowel disease and ovarian cancer (7, 8). As a cellular adhesion molecule (CAM), MCAM functions as a molecular mediator to facilitate inter-cellular interactions of homotypic or heterotypic cells, or to intervene in interactions of cell-to-extracellular matrix for responding to physiological signal (9). MCAM has also been shown to be the functional ligand for Galectin-3 on endothelial cell surfaces (9).

References
  1. Lehmann, J.M. et al. (1989) Proc Natl Acad Sci U.S.A. 86:9891.
  2. Alais, S. et al. (2001) J. Cell Sci. 114:1847.
  3. Shih, I.M. et al. (1994) Am J Pathol. 145:837.
  4. Schrage, A. et al. (2008) Histochem Cell Biol. 129:441.
  5. Wang Z and Yan X. (2013) Cancer Lett. 330:150.
  6. Bardin, N. et al. (2006) Inflamm Bowel Dis. 12:16.
  7. Xing, S. et al. (2014) Am J Pathol. 184:1604.
  8. Ma, X. et al. (2017) Oncol Lett. 13:1681.
  9. Colomb F, et al. (2017) J Biol Chem. 292:8381.
Long Name
Melanoma Cell Adhesion Molecule
Entrez Gene IDs
4162 (Human); 84004 (Mouse); 78967 (Rat)
Alternate Names
CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

Citations for Recombinant Human MCAM/CD146 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CD146/Soluble CD146 Pathway Is a Novel Biomarker of Angiogenesis and Inflammation in Proliferative Diabetic Retinopathy
    Authors: AM Abu El-Asr, MI Nawaz, A Ahmad, MM Siddiquei, E Allegaert, PW Gikandi, G De Hertogh, G Opdenakker
    Investigative Ophthalmology & Visual Science, 2021;62(9):32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application.
    Authors: Malara N, Trunzo V, Musolino G, Aprigliano S, Rotta G, Macrina L, Limongi T, Gratteri S, Di Fabrizio E, Renzulli A, Fini M, Mollace V
    Int J Cardiol Heart Vasc, 0;6(0):48-53.

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