hh Recombinant Human PDGF-BB Biotinylated Protein, CF BT220-010/CF: R&D Systems

Recombinant Human PDGF-BB Biotinylated Protein, CF

(1 citations)   
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.
  • Source
    E. coli-derived Ser82-Thr190
  • Accession #
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    12 kDa
  • SDS-PAGE
    12 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
BT220/CF
 
BT220
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Both Biotinylated Recombinant Human PDGF-BB (Catalog # BT220/CF) and unlabeled Recombinant Human PDGF-BB (Catalog # 220-BB/CF) stimulate NR6R‑3T3 mouse fibroblast cell proliferation. The ED50 for this effect is 1.5-6 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional.
Background: PDGF-BB
Platelet-Derived Growth Factor (PDGF)-BB is synthesized as a 35 kDa, 241 amino acid (aa) prepro-precurser. It contains a signal peptide, an N-terminal prodomain, a mature region, and a C-terminal prodomain (1-4). The propercursor is initially dimerized and then intracellulary processed twice. The N-terminal prodomain is cleaved first, followed by cleavage of the C-terminal prodomain. The resulting mature region is 16-17 kDa in size (or 29-32 kDa as a homodimer) (4). Mature human PDGF-B shares 89% aa sequence identity with mouse mature PDGF-B. PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation (4, 5). It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization (6, 7). The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta (8, 9). PDGF-BB has been shown to bind the alpha alpha homodimer, alpha beta heterodimer, and the beta beta  homodimer in vitro, and act through the beta beta homodimer in vivo (8, 10).
  • References:
    1. Rao, C.D. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2392.
    2. Kaetzel, D.M. et al. (1996) Biochim. Biophys. Acta. 1298:250.
    3. Ostman, A. et al. (1992) J. Cell Biol. 118:509.
    4. Siegfried, G. et al. (2005) Oncogene 24:6925.
    5. Kreja, L. et al. (2010) J. Cell. Biochem. 109:347.
    6. van Steensel, L. et al. (2012) J. Clin. Endocrinol. Metab. 97:E400.
    7. Barrientos, S. et al. (2008) Wound Repair Regen. 16:585.
    8. Andrae, J. et al. (2008) Genes Dev. 22:1276.
    9. Heldin, C.H. and B. Westermark (1999) Physiol. Rev. 79:1283.
    10. Li, X. and U. Eriksson (2003) Cytokine Growth Factor Rev. 14:91.
  • Long Name:
    Platelet-derived Growth Factor BB
  • Entrez Gene IDs:
    5155 (Human); 24628 (Rat)
  • Alternate Names:
    PDGFBB; PDGF-BB
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Silibinin Inhibits Platelet-Derived Growth Factor-Driven Cell Proliferation via Downregulation of N-Glycosylation in Human Tenon's Fibroblasts in a Proteasome-Dependent Manner
    PLoS ONE, 2016;11(12):e0168765.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
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