Recombinant Human Poly-SUMO2 Hydrolysis Resistant (3-8), CF
Recombinant Human Poly-SUMO2 Hydrolysis Resistant (3-8), CF Summary
Each SUMO2 contains a Pro substitution at position 90.
32 kDa (SUMO23), 42 kDa (SUMO24), 53 kDa (SUMO25), 63 kDa (SUMO26), 74 kDa (SUMO27), 85 kDa (SUMO28)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM DTT|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Sentrin protease (SENP)-resistant poly-SUMO2 chains can be used to investigate mechanisms of chain recognition by SUMO-targeted ubiquitin ligases (STUbl's) or other proteins that contain SUMO-interacting domains (SIM's). These K11-linked chains are formed enzymatically with recombinant SUMO2 containing a mutation of glutamine 90 to proline, which renders them approximately 500-fold more resistant to disassembly by SENP's than are wild-type chains. Mono- and di-SUMO2 have been removed from the chain mixture.
- Bekes, M., et al. (2011), J. Biol. Chem. 286: 10238
- Bekes, M., et.al. (2013) Cell Rep. 5: 826
- Bylebyl G.R., et al. (2003) J. Biol. Chem. 278: 44113
- Dohmen R. J. (2004) Biochem. Biophys. Acta. 1695:113
- Johnson E. (2004) Ann. Rev. Biochem. 73: 355
- Tatham M.H., et al. (2001) J. Biol. Chem. 276: 35368
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