Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein, CF

R&D Systems | Catalog # AVI4234

Biotinylated
R&D Systems
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein (AVI4234)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q04609.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity, Enzyme Activity
View all PSMA/FOLH1/NAALADase I Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human PSMA/FOLH1/NAALADase I protein
Avi-tag HHHHHH  Human PSMA/FOLH1/NAALADase I
(Lys44-Ala750)
Accession # Q04609.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly

Predicted Molecular Mass

83 kDa

SDS-PAGE

94-107 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human PSMA/FOLH1 Affinity Purified Polyclonal Antibody (Catalog # AF4234) is immobilized  at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human PSMA/FOLH1/NAALADase I His-tag Avi-tag with an ED50 of 0.01-0.08 μg/mL.

Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde.
The specific activity is > 250 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein, CF

Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein Binding Activity

Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein Binding Activity

When Human PSMA/FOLH1/NAALADase I Affinity Purified Polyclonal Antibody (AF4234) is immobilized at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human PSMA/FOLH1/NAALADase I His-tag Avi-tag with an ED50 of 0.01-0.08 μg/mL.

Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein SDS-PAGE

Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human PSMA/FOLH1/NAALADase I His-tag Avi-tag (Catalog # AVI4234) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~100 kDa.

Formulation, Preparation, and Storage

AVI4234
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: PSMA/FOLH1/NAALADase I

Prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1,2). PMSA has a short cytosolic N-terminal domain, a single membrane-spanning segment, and an extracellular region that is composed of a protease domain, apical domain, and C-terminal domain (3). The extracellular domains all contribute to substrate recognition. The protein forms an active homodimer reliant on interactions between amino-acid side chains and glycosylation (3,4). PSMA is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, and N-acetylated-alpha-linked acidic dipeptidase-1 (NAALADase1). PSMA activity plays a role in tumor angiogenesis making it not only a tumor marker, but a therapeutic target in cancers including prostate cancer (5). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu (NAAG) to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity such as stroke, schizophrenia, Alzheimer's, and amyotrophic lateral sclerosis (6,7,8). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (8). Upregulation of PSMA is present in inflammatory bowel disease, Crohn's disease, and ulcerative colitis where pharmacological inhibition has shown amelioration of clinical symptoms pertaining to these diseases in mice (5).

References

  1. Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
  2. Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
  3. Mesters, J.R. et al. (2006) EMBO J. 25:1375.
  4. Shulke, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12590.
  5. Vornov, J.J. et al. (2019) Neurochem. Res. 45:1256. 
  6. Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
  7. Neale, J. J and T. Yamamoto. (2020) Prog. Neurobiol. 184:101722.
  8. Heston, W.D. (1997) Urology 49:104.

Long Name

Prostate-specific Membrane Antigen

Alternate Names

FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I

Entrez Gene IDs

2346 (Human); 53320 (Mouse); 85309 (Rat); 101866140 (Cynomolgus Monkey)

Gene Symbol

FOLH1

UniProt

Q04609.1

Additional PSMA/FOLH1/NAALADase I Products

Product Documents for Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein, CF

For research use only

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