Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein, CF

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Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein Binding Activity
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Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Human PSMA/FOLH1 Affinity Purified Polyclonal Antibody (Catalog # AF4234) is immobilized  at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human PSMA/FOLH1/NAALADase I His-tag Avi-tag with an ED50 of 0.01-0.08 μg/mL. Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is > 250 pmol/min/μg, as measured under the described conditions.
Chinese Hamster Ovary cell line, CHO-derived human PSMA/FOLH1/NAALADase I protein
Accession # Q04609.1
Accession #
N-terminal Sequence
Structure / Form
Biotinylated via Avi-tag
Predicted Molecular Mass
83 kDa
94-107 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein Binding Activity View Larger

When Human PSMA/FOLH1/NAALADase I Affinity Purified Polyclonal Antibody (AF4234) is immobilized at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human PSMA/FOLH1/NAALADase I His-tag Avi-tag with an ED50 of 0.01-0.08 μg/mL.

SDS-PAGE Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human PSMA/FOLH1/NAALADase I His-tag Avi-tag (Catalog # AVI4234) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~100 kDa.

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Background: PSMA/FOLH1/NAALADase I

Prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1,2). PMSA has a short cytosolic N-terminal domain, a single membrane-spanning segment, and an extracellular region that is composed of a protease domain, apical domain, and C-terminal domain (3). The extracellular domains all contribute to substrate recognition. The protein forms an active homodimer reliant on interactions between amino-acid side chains and glycosylation (3,4). PSMA is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, and N-acetylated-alpha-linked acidic dipeptidase-1 (NAALADase1). PSMA activity plays a role in tumor angiogenesis making it not only a tumor marker, but a therapeutic target in cancers including prostate cancer (5). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu (NAAG) to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity such as stroke, schizophrenia, Alzheimer's, and amyotrophic lateral sclerosis (6,7,8). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (8). Upregulation of PSMA is present in inflammatory bowel disease, Crohn's disease, and ulcerative colitis where pharmacological inhibition has shown amelioration of clinical symptoms pertaining to these diseases in mice (5).

  1. Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
  2. Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
  3. Mesters, J.R. et al. (2006) EMBO J. 25:1375.
  4. Shulke, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12590.
  5. Vornov, J.J. et al. (2019) Neurochem. Res. 45:1256. 
  6. Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
  7. Neale, J. J and T. Yamamoto. (2020) Prog. Neurobiol. 184:101722.
  8. Heston, W.D. (1997) Urology 49:104.
Long Name
Prostate-specific Membrane Antigen
Entrez Gene IDs
2346 (Human); 53320 (Mouse); 85309 (Rat); 101866140 (Cynomolgus Monkey)
Alternate Names
Cell growth-inhibiting gene 27 protein; cell growth-inhibiting protein 27; EC; FGCP; folate hydrolase (prostate-specific membrane antigen) 1; Folate hydrolase 1; FOLH1; FOLHNAALADase I; Folylpoly-gamma-glutamate carboxypeptidase; GCP2; GCPII; GCPIImGCP; glutamate carboxylase II; glutamate carboxypeptidase 2; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; mopsm; NAALAD1; NAALAD1N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; NAALAdase; N-acetylated alpha-linked acidic dipeptidase 1; prostate specific membrane antigen variant F; Prostate-specific membrane antigen; PSMA; PSMAFGCP; PSMPteroylpoly-gamma-glutamate carboxypeptidase


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