Human PSMA/FOLH1/NAALADase I Antibody

Catalog # Availability Size / Price Qty
AF4234
AF4234-SP
Detection of Human PSMA/FOLH1/ NAALADase I by Western Blot.
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Human PSMA/FOLH1/NAALADase I Antibody Summary

Species Reactivity
Human
Specificity
Detects human PSMA/FOLH1/NAALADase I in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse NAALADase II is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human PSMA/FOLH1/NAALADase I
Lys44-Ala750
Accession # Q04609
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB42341).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PSMA/FOLH1 DuoSet ELISA Kit (Catalog # DY4234-05) for convenient development of a sandwich ELISA.

 
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human PSMA/FOLH1/ NAALADase I by Western Blot. View Larger

Detection of Human PSMA/FOLH1/ NAALADase I by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human PSMA/FOLH1/ NAALADase I Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PSMA/FOLH1/NAALADase I at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of PSMA/FOLH1/ NAALADase I in LnCAP Human Cell Line by Flow Cytometry. View Larger

Detection of PSMA/FOLH1/ NAALADase I in LnCAP Human Cell Line by Flow Cytometry. LnCAP human prostate cancer cell line was stained with Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4234, filled histo-gram) or control antibody (Catalog # 5-001-A, open histo-gram), followed by Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Immunohistochemistry PSMA/FOLH1/NAALADase I in Human Prostate Cancer Tissue. View Larger

PSMA/FOLH1/NAALADase I in Human Prostate Cancer Tissue. PSMA/FOLH1/NAALADase I was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4234) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and extracellular space. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Human PSMA/FOLH1/NAALADase I ELISA Standard Curve. Recombinant Human PSMA/FOLH1/NAALADase I protein was serially diluted 2-fold and captured by Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (MAB42341) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4234) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSMA/FOLH1/NAALADase I

Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).

References
  1. Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
  2. Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA 93:749.
  3. Jackson, P.F. and B.S. Slusher (2001) Curr. Med. Chem. 8:949.
  4. Heston, W.D. (1997) Urology 49:104.
Long Name
Prostate-specific Membrane Antigen
Entrez Gene IDs
2346 (Human); 53320 (Mouse); 85309 (Rat); 101866140 (Cynomolgus Monkey)
Alternate Names
Cell growth-inhibiting gene 27 protein; cell growth-inhibiting protein 27; EC 3.4.17.21; FGCP; folate hydrolase (prostate-specific membrane antigen) 1; Folate hydrolase 1; FOLH1; FOLHNAALADase I; Folylpoly-gamma-glutamate carboxypeptidase; GCP2; GCPII; GCPIImGCP; glutamate carboxylase II; glutamate carboxypeptidase 2; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; mopsm; NAALAD1; NAALAD1N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; NAALAdase; N-acetylated alpha-linked acidic dipeptidase 1; prostate specific membrane antigen variant F; Prostate-specific membrane antigen; PSMA; PSMAFGCP; PSMPteroylpoly-gamma-glutamate carboxypeptidase

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Citation for Human PSMA/FOLH1/NAALADase I Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Diagnostic potential of serum extracellular vesicles expressing prostate-specific membrane antigen in urologic malignancies
    Authors: K Kawakami, Y Fujita, T Kato, K Horie, T Koie, K Umezawa, H Tsumoto, Y Miura, Y Katagiri, T Miyazaki, I Ohsawa, K Mizutani, M Ito
    Scientific Reports, 2021;11(1):15000.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Capture

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