Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Cited:

Capture Assay, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PSMA/FOLH1/NAALADase I Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human PSMA/FOLH1/NAALADase I
Lys44-Ala750
Accession # Q04609

Specificity

Detects human PSMA/FOLH1/NAALADase I in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse NAALADase II is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PSMA/FOLH1/NAALADase I Antibody

Detection of Human PSMA/FOLH1/ NAALADase I antibody by Western Blot.

Detection of Human PSMA/FOLH1/ NAALADase I by Western Blot.

Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human PSMA/FOLH1/ NAALADase I Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PSMA/FOLH1/NAALADase I at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of PSMA/FOLH1/ NAALADase I antibody in LnCAP Human Cell Line antibody by Flow Cytometry.

Detection of PSMA/FOLH1/ NAALADase I in LnCAP Human Cell Line by Flow Cytometry.

LnCAP human prostate cancer cell line was stained with Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4234, filled histo-gram) or control antibody (Catalog # 5-001-A, open histo-gram), followed by Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

PSMA/FOLH1/NAALADase I antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

PSMA/FOLH1/NAALADase I in Human Prostate Cancer Tissue.

PSMA/FOLH1/NAALADase I was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4234) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and extracellular space. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Human PSMA/FOLH1/NAALADase I ELISA Standard Curve.

Recombinant Human PSMA/FOLH1/NAALADase I protein was serially diluted 2-fold and captured by Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (MAB42341) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4234) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human PSMA/FOLH1/NAALADase I Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB42341).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PSMA/FOLH1 DuoSet ELISA Kit (Catalog # DY4234-05) for convenient development of a sandwich ELISA.

Flow Cytometry

2.5 µg/106 cells
Sample: LnCAP human prostate cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

0.2 µg/mL
Sample: Human prostate tissue

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PSMA/FOLH1/NAALADase I

Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).

References

  1. Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
  2. Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA 93:749.
  3. Jackson, P.F. and B.S. Slusher (2001) Curr. Med. Chem. 8:949.
  4. Heston, W.D. (1997) Urology 49:104.

Long Name

Prostate-specific Membrane Antigen

Alternate Names

FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I

Entrez Gene IDs

2346 (Human); 53320 (Mouse); 85309 (Rat); 101866140 (Cynomolgus Monkey)

Gene Symbol

FOLH1

UniProt

Q04609

Additional PSMA/FOLH1/NAALADase I Products

Product Documents for Human PSMA/FOLH1/NAALADase I Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PSMA/FOLH1/NAALADase I Antibody

For research use only

Citations for Human PSMA/FOLH1/NAALADase I Antibody

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Protocols

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