Recombinant Human R-Spondin 1 Biotinylated Protein, CF

  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is typically 5-30 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Arg31-Ala263
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg31
  • Structure / Form
    Biotinylated protein via sugars
  • Predicted Molecular Mass
    26 kDa (unlabeled)
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
BT4645/CF
 
BT4645
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Both Biotinylated Recombinant Human R-Spondin 1 (Catalog # BT4645/CF) and unlabeled Recombinant Human R-Spondin 1 (Catalog #
4645-RS) induces activation of beta -catenin response in Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 5-30 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The similarity in activity highlights that the biotinylated protein is fully functional.
Background: R-Spondin 1
R-Spondin 1 (RSPO1), also known as Cristin 3, is a 27 kDa secreted protein in the R-Spondin family of Wnt/ beta -catenin signaling regulators (1). These proteins contain two adjacent cysteine-rich furin-like domains followed by a thrombospondin (TSP-1) motif and a region rich in basic residues. Mature human R-Spondin 1 shares 87% amino acid sequence identity with mouse and rat R-Spondin 1 (2). Alternative splicing generates additional isoforms that have a substituted N-terminus or lack the TSP-1 domain. R-Spondin 1 enhances canonical Wnt/ beta -catenin signaling by competing with the Wnt antagonist Dkk-1, binding to Frizzled-8, Kremen, LRP-6, Lgr4, Lgr5, and Lgr6, and enhancing cell surface availability of Wnt receptors (3-11). R-Spondin 1 functions in dorsal neural tube development (12) as well as male and female germ cell development (7, 10, 13). It also induces bone formation (6), intestinal crypt cell proliferation (14), angiogenesis (7, 15), and insulin secretion from pancreatic beta cells (11).
  • References:
    1. Jin, Y.R. and J.K. Yoon (2012) Int. J. Biochem. Cell Biol. 44:2278.
    2. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
    3. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
    4. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
    5. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
    6. Kronke, G. et al. (2010) Arthritis Rheum. 62:2303.
    7. Caruso, M. et al. (2015) PLoS One 10:e0124213.
    8. Hao, H.-X. et al. (2012) Nature 485:195.
    9. de Lau, W. et al. (2011) Nature 476:293.
    10. Chassot, A.-A. et al. (2011) PLoS One 6:e25641.
    11. Wong, V.S.C et al. (2010) J. Biol. Chem. 285:21292.
    12. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
    13. Chassost, A.-A. et al. (2012) Development 139:4461.
    14. Kim, K.-A. et al. (2005) Science 309:1256.
    15. Gore, A.V. et al. (2011) Development 138:4875.
  • Long Name:
    Roof Plate-specific Spondin 1
  • Entrez Gene IDs:
    284654 (Human); 192199 (Mouse)
  • Alternate Names:
    Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; hRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1
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