Recombinant Human R-Spondin 1 Protein Summary
Product Specifications
Ser21-Ala263
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4645-RS
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
4645-RS/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Images

Recombinant Human R-Spondin 1 (Catalog # 4645-RS), in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN); 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay (blue). The activity is approximately 7-fold greater than the competitor's R-Spondin 1 (red).

1 µg/lane of Recombinant Human R-Spondin 1 was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 39 kDa.
Reconstitution Calculator
Background: R-Spondin 1
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).
Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively (14). Over aa 21-263, human R-Spondin 1 shares 89%, 87%, 92%, 91%, 91% and 89% aa identity with mouse, rat, horse, dog, goat, and cow RSPO-1, respectively.
- Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
- Kim, K.-A. et al. (2006) Cell Cycle 5:23.
- Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
- Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
- Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
- Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
- Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
- Parma, P. et al. (2006) Nat. Genet. 38:1304.
- Kim, K.-A. et al. (2005) Science 309:1256.
- Zhao, J. et al. (2007) Gastroenterology 132:1331.
- Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
- Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
- Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
- UniProt # Q2MKA7.
Citations for Recombinant Human R-Spondin 1 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
Nat. Biotechnol., 2020;0(0):.
Species: Human
Sample Types: cell culture
Applications: Cell Culture -
Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
Authors: S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi
Sci Rep, 2020;10(1):5989.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells
Authors: Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban
Nat. Immunol., 2020;21(3):274-286.
Species: Human
Sample Types: Cells
Applications: Cell Culture -
Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs
Authors: M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie
PLoS ONE, 2020;15(3):e0229445.
Species: Human
Sample Types: Whole Cells
Applications: IHC Control -
Expansion of Human iPSC-Derived Ureteric Bud Organoids with Repeated Branching Potential
Authors: SI Mae, M Ryosaka, S Sakamoto, K Matsuse, A Nozaki, M Igami, R Kabai, A Watanabe, K Osafune
Cell Rep, 2020;32(4):107963.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
Patient-derived ovarian cancer organoids capture the genomic profiles of primary tumours applicable for drug sensitivity and resistance testing
Authors: Y Nanki, T Chiyoda, A Hirasawa, A Ookubo, M Itoh, M Ueno, T Akahane, K Kameyama, W Yamagami, F Kataoka, D Aoki
Sci Rep, 2020;10(1):12581.
Species: Human
Sample Types: Organoid
Applications: Bioassay -
Standardized GMP-compliant scalable production of human pancreas organoids
Authors: M Dossena, R Piras, A Cherubini, M Barilani, E Dugnani, F Salanitro, T Moreth, F Pampaloni, L Piemonti, L Lazzari
Stem Cell Res Ther, 2020;11(1):94.
Species: Human
Sample Types: Whole Tissue
Applications: Tissue Culture -
Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells
Authors: G Levin, BAA Koga, GG Belchior, ACO Carreira, MC Sogayar
BMC Biotechnol., 2020;20(1):5.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
NOD2 Supports Crypt Survival and Epithelial Regeneration after Radiation-Induced Injury
Authors: C Lee, C Choi, HS Kang, SW Shin, SY Kim, HC Park, SN Hong
Int J Mol Sci, 2019;20(17):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model
Authors: B Zwarycz, AD Gracz, KR Rivera, IA Williamson, LA Samsa, J Starmer, MA Daniele, L Salter-Cid, Q Zhao, ST Magness
Cell Mol Gastroenterol Hepatol, 2019;7(1):1-17.
Species: Mouse
Sample Types: Organoids
Applications: Bioassay -
Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells
Authors: S Konagaya, H Iwata
Sci Rep, 2019;9(1):640.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue
Authors: OC Tysoe, AW Justin, T Brevini, SE Chen, KT Mahbubani, AK Frank, H Zedira, E Melum, K Saeb-Parsy, AE Markaki, L Vallier, F Sampazioti
Nat Protoc, 2019;14(6):1884-1925.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Organoid culture media formulated with growth factors of defined cellular activity
Authors: M Urbischek, H Rannikmae, T Foets, K Ravn, M Hyvönen, M de la Roch
Sci Rep, 2019;9(1):6193.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling
Authors: HLX Wong, HY Qin, SW Tsang, X Zuo, S Che, CFW Chow, X Li, HT Xiao, L Zhao, T Huang, CY Lin, HY Kwan, T Yang, FM Longo, A Lyu, ZX Bian
Nat Commun, 2019;10(1):1745.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling
Authors: S Akbari, GG Sevinç, N Ersoy, O Basak, K Kaplan, K Sevinç, E Ozel, B Sengun, E Enustun, B Ozcimen, A Bagriyanik, N Arslan, TT Önder, E Erdal
Stem Cell Reports, 2019;13(4):627-641.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Mist1 Expression is Required for Paneth Cell Maturation
Authors: CM Dekaney, S King, B Sheahan, J Cortes
Cell Mol Gastroenterol Hepatol, 2019;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Patient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders
Authors: K Shik Mun, K Arora, Y Huang, F Yang, S Yarlagadda, Y Ramananda, M Abu-El-Hai, JJ Palermo, BN Appakalai, JD Nathan, AP Naren
Nat Commun, 2019;10(1):3124.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Endometrial Axin2+ Cells Drive Epithelial Homeostasis, Regeneration, and Cancer following Oncogenic Transformation
Authors: SM Syed, M Kumar, A Ghosh, F Tomasetig, A Ali, RM Whan, D Alterman, PS Tanwar
Cell Stem Cell, 2019;26(1):64-80.e13.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay -
USP9X Deubiquitylates DVL2 to Regulate WNT Pathway Specification
Authors: CP Nielsen, KK Jernigan, NL Diggins, DJ Webb, JA MacGurn
Cell Rep, 2019;28(4):1074-1089.e5.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion
Authors: PG Boone, LK Rochelle, JD Ginzel, V Lubkov, WL Roberts, PJ Nicholls, C Bock, ML Flowers, RJ von Furste, BR Stripp, P Agarwal, AD Borowsky, RD Cardiff, LS Barak, MG Caron, HK Lyerly, JC Snyder
Nat Commun, 2019;10(1):5490.
Species: Mouse
Sample Types: Organoids
Applications: Cell Culture -
PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery
Authors: R Cruz-Acuña, M Quirós, S Huang, D Siuda, JR Spence, A Nusrat, AJ García
Nat Protoc, 2018;0(0):.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA
Authors: YC Chen, CL Tsai, YH Wei, YT Wu, WT Hsu, HC Lin, YC Hsu
Cell Death Dis, 2018;9(4):437.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Culture and characterization of chicken small intestinal crypts
Authors: J Li, J Li, SY Zhang, RX Li, X Lin, YL Mi, CQ Zhang
Poult. Sci., 2018;0(0):.
Species: Avian - Chicken
Sample Types: Whole Cells
Applications: Bioassay -
Cell-type specific potent Wnt signaling blockade by bispecific antibody
Authors: NK Lee, Y Zhang, Y Su, S Bidlingmai, DW Sherbenou, KD Ha, B Liu
Sci Rep, 2018;8(1):766.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
R-spondins can potentiate WNT signaling without LGRs
Authors: AM Lebensohn, R Rohatgi
Elife, 2018;7(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Differential activities and mechanisms of the four R-Spondins in potentiating Wnt/?-catenin signaling
Authors: S Park, J Cui, WA Yu, L Wu, K Carmon, QJ Liu
J. Biol. Chem., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA
Authors: MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak
Cell Rep, 2018;25(13):3811-3827.e7.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer
Authors: JD Barros-Sil, DE Linn, I Steiner, G Guo, A Ali, H Pakula, G Ashton, I Peset, M Brown, NW Clarke, RT Bronson, GC Yuan, SH Orkin, Z Li, E Baena
Cell Rep, 2018;25(12):3504-3518.e6.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A surgical orthotopic organoid transplantation approach in mice to visualize and study colorectal cancer progression
Authors: A Fumagalli, SJE Suijkerbui, H Begthel, E Beerling, KC Oost, HJ Snippert, J van Rheene, J Drost
Nat Protoc, 2018;13(2):235-247.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
R-Spondin1/LGR5 Activates TGF? Signaling and Suppresses Colon Cancer Metastasis
Authors: X Zhou, L Geng, D Wang, H Yi, G Talmon, J Wang
Cancer Res., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Higher-Order Kidney Organogenesis from Pluripotent Stem Cells
Authors: A Taguchi, R Nishinakam
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Establishment of a refined culture method for rat colon organoids
Authors: H Isshiki, Y Arimura, K Nagaishi, K Kawakami, K Onodera, K Yamashita, Y Naishiro, M Fujimiya, K Imai, Y Shinomura
Biochem. Biophys. Res. Commun., 2017;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Directional delivery of RSPO1 by mesenchymal stem cells ameliorates radiation-induced intestinal injury
Authors: W Chen, S Ju, T Lu, Y Xu, X Zheng, H Wang, Y Ge, S Ju
Cytokine, 2017;95(0):27-34.
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Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway
Authors: Y Gao, A Cai, H Xi, J Li, W Xu, Y Zhang, K Zhang, J Cui, X Wu, B Wei, L Chen
Stem Cell Res Ther, 2017;8(1):98.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
P2X7 receptor-dependent tuning of gut epithelial responses to infection
Authors: SW Huang, C Walker, J Pennock, K Else, W Muller, MJ Daniels, C Pellegrini, D Brough, G Lopez-Cast, SM Cruickshan
Immunol. Cell Biol, 2017;95(2):178-188.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Id2 controls specification of Lgr5+ intestinal stem cell progenitors during gut development
Authors: L Nigmatulli, M Norkin, MM Dzama, B Messner, S Sayols, N Soshnikova
EMBO J., 2017;36(7):869-885.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay -
Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor
Authors: SH Han, S Shim, MJ Kim, HY Shin, WS Jang, SJ Lee, YW Jin, SS Lee, SB Lee, S Park
World J. Gastroenterol, 2017;23(6):964-975.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids.
Authors: Rouch J, Scott A, Lei N, Solorzano-Vargas R, Wang J, Hanson E, Kobayashi M, Lewis M, Stelzner M, Dunn J, Eckmann L, Martin M
PLoS ONE, 2016;11(1):e0148216.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates beta-Catenin Signaling and Tumorigenesis in Multiple Cancer Types.
Authors: Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee-Hoskins C, Ma S, Fischer M, Shah J, Wei J, Ji M, Lam A, Stroud M, Yen W, Yeung P, Cancilla B, O'Young G, Wang M, Kapoun A, Lewicki J, Hoey T, Gurney A
Cancer Res, 2016;76(3):713-23.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Intestinal enteroids model GUCY2C-dependent secretion induced by heat-stable enterotoxins
Infect Immun, 2016;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Novel Bi-specific Domain Antibody to LRP6 Inhibits Wnt and R-spondin Ligand-induced Wnt Signaling and Tumor Growth
Mol Cancer Res, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
Sci Rep, 2016;6(0):36795.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note.
Authors: James M, Howells L, Karmokar A, Higgins J, Greaves P, Cai H, Dennison A, Metcalfe M, Garcea G, Lloyd D, Berry D, Steward W, Brown K
PLoS ONE, 2015;10(2):e0117776.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inner ear hair cell-like cells from human embryonic stem cells.
Authors: Ronaghi, Mohammad, Nasr, Marjan, Ealy, Megan, Durruthy-Durruthy, Robert, Waldhaus, Joerg, Diaz, Giovanni, Joubert, Lydia-Ma, Oshima, Kazuo, Heller, Stefan
Stem Cells Dev, 2014;23(11):1275-84.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer.
Authors: Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X
Nat Commun, 2014;5(0):3149.
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Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.
Authors: Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A
J Immunol, 2014;193(1):439-51.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Hippo pathway activity influences liver cell fate.
Authors: Yimlamai D, Christodoulou C, Galli G, Yanger K, Pepe-Mooney B, Gurung B, Shrestha K, Cahan P, Stanger B, Camargo F
Cell, 2014;157(6):1324-38.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Intestinal subepithelial myofibroblasts support the growth of intestinal epithelial stem cells.
Authors: Lei N, Jabaji Z, Wang J, Joshi V, Brinkley G, Khalil H, Wang F, Jaroszewicz A, Pellegrini M, Li L, Lewis M, Stelzner M, Dunn J, Martin M
PLoS ONE, 2014;9(1):e84651.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium.
Authors: Jabaji Z, Brinkley G, Khalil H, Sears C, Lei N, Lewis M, Stelzner M, Martin M, Dunn J
PLoS ONE, 2014;9(9):e107814.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.
Authors: Carmon K, Gong X, Yi J, Thomas A, Liu Q
Proc Natl Acad Sci U S A, 2014;111(13):E1221-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway.
Authors: Guezguez A, Pare F, Benoit Y, Basora N, Beaulieu J
Exp Cell Res, 2014;322(2):355-64.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
Authors: Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, Markmann J, Miyazaki S, Miyazaki J, Szot G, Bottino R, Kim S
Elife, 2013;2(0):e00940.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.
Authors: Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F
Nature, 2012;485(7397):195-200.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Role of Gadd45a in Wip1-dependent regulation of intestinal tumorigenesis.
Cell Death Differ., 2012;19(11):1761-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Authors: Spence JR, Mayhew CN, Rankin SA
Nature, 2011;470(7332):105-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling.
Authors: Lebensohn A, Dubey R, Neitzel L, Tacchelly-Benites O, Yang E, Marceau C, Davis E, Patel B, Bahrami-Nejad Z, Travaglini K, Ahmed Y, Lee E, Carette J, Rohatgi R
Elife, 0;5(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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Reviews for Recombinant Human R-Spondin 1 Protein
Average Rating: 4.5 (Based on 4 Reviews)
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Reason for Rating: stable product when used as described; produces repeatable results for differentiation
Reason for Rating: Protein performed as expected in assay
Wnt3a biology and cell growth. Use HEK Wnt3a reporter cell line.