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Recombinant Human R-Spondin 1 Protein

Catalog #: 4645-RS
89 Citations 6 Reviews Datasheet / COA / SDS
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Carrier Free

Catalog # Availability Size / Price Qty
4645-RS-025/CF
4645-RS-01M/CF
4645-RS-100/CF

With Carrier

Catalog # Availability Size / Price Qty
4645-RS-025
4645-RS-250
4645-RS-01M
4645-RS-100
Recombinant Human R-Spondin 1 Protein Bioactivity
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Product Details
Citations (89)
FAQs
Supplemental Products
Reviews (6)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human R-Spondin 1 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein
Ser21-Ala263
Accession #
Q2MKA7
N-terminal Sequence
Analysis
Ser21 & Arg31
Predicted Molecular Mass
25.6 kDa
SDS-PAGE
39 kDa, reducing conditions

Product Datasheets

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4645-RS (with carrier)

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COA
SDS

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4645-RS/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4645-RS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4645-RS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human R-Spondin 1 Protein Bioactivity View Larger

Recombinant Human R-Spondin 1 (Catalog # 4645-RS), in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN); 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay (blue). The activity is approximately 7-fold greater than the competitor's R-Spondin 1 (red).

Bioactivity View Larger

The lot-to-lot consistency of Recombinant Human R-Spondin 1 (Catalog # 4645-RS) was assessed by testing the ability of three independent lots of the protein to stimulate activation of beta-Catenin using a TOPflash beta-Catenin/TCF reporter assay in the HEK293T human kidney cell line, in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (1324-WN). Each trace on the graph represents data obtained from Recombinant Human R-Spondin 1 from a different manufacturing run. The ED50 for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.

SDS-PAGE Recombinant Human R-Spondin 1 Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Human R-Spondin 1 was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 39 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).

Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively (14). Over aa 21-263, human R-Spondin 1 shares 89%, 87%, 92%, 91%, 91% and 89% aa identity with mouse, rat, horse, dog, goat, and cow RSPO-1, respectively.

References
  1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  5. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  6. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  7. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
  8. Parma, P. et al. (2006) Nat. Genet. 38:1304.
  9. Kim, K.-A. et al. (2005) Science 309:1256.
  10. Zhao, J. et al. (2007) Gastroenterology 132:1331.
  11. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
  12. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  13. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
  14. UniProt # Q2MKA7.
Long Name
Roof Plate-specific Spondin 1
Entrez Gene IDs
284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)
Alternate Names
Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1

Citations for Recombinant Human R-Spondin 1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

89 Citations: Showing 1 - 10
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  1. Generation and cryopreservation of feline oviductal organoids
    Authors: RE Thompson, MA Meyers, C Premananda, FK Hollinshea
    Theriogenology, 2022;196(0):167-173.
    Species: Feline
    Sample Types: Organoids
    Applications: Bioassay
  2. Identification of trypsin-degrading commensals in the large intestine
    Authors: Y Li, E Watanabe, Y Kawashima, DR Plichta, Z Wang, M Ujike, QY Ang, R Wu, M Furuichi, K Takeshita, K Yoshida, K Nishiyama, SM Kearney, W Suda, M Hattori, S Sasajima, T Matsunaga, X Zhang, K Watanabe, J Fujishiro, JM Norman, B Olle, S Matsuyama, H Namkoong, Y Uwamino, M Ishii, K Fukunaga, N Hasegawa, O Ohara, RJ Xavier, K Atarashi, K Honda
    Nature, 2022;609(7927):582-589.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  3. Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids
    Authors: S Kim, S Min, YS Choi, SH Jo, JH Jung, K Han, J Kim, S An, YW Ji, YG Kim, SW Cho
    Nature Communications, 2022;13(1):1692.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids
    Authors: J Jeong, TH Kim, M Kim, YK Jung, KS Kim, S Shim, H Jang, WI Jang, SB Lee, D Choi
    Biology, 2022;11(4):.
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Bioassay
  5. Establishment of bovine 3D enteroid-derived 2D monolayers
    Authors: KM Sutton, B Orr, J Hope, SR Jensen, L Vervelde
    Veterinary research, 2022;53(1):15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Spatiotemporal reprogramming of differentiated cells underlies regeneration and neoplasia in the intestinal epithelium
    Authors: T Higa, Y Okita, A Matsumoto, S Nakayama, T Oka, O Sugahara, D Koga, S Takeishi, H Nakatsumi, N Hosen, S Robine, MM Taketo, T Sato, KI Nakayama
    Nature Communications, 2022;13(1):1500.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. Renalase and its receptor, PMCA4b, are expressed in the placenta throughout the human gestation
    Authors: M Wang, T Silva, JM Toothaker, BT McCourt, C Shugrue, G Desir, F Gorelick, L Konnikova
    Scientific Reports, 2022;12(1):4953.
    Species: Human
    Sample Types: Organoid
    Applications: Cell Culture
  8. Desmoplakin maintains transcellular keratin scaffolding and protects from intestinal injury
    Authors: A Gross, B Zhou, L Bewersdorf, N Schwarz, GM Schacht, P Boor, K Hoeft, B Hoffmann, E Fuchs, R Kramann, R Merkel, RE Leube, P Strnad
    Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  9. Comparative Analysis of Patient-Matched PDOs Revealed a Reduction in OLFM4-Associated Clusters in Metastatic Lesions in Colorectal Cancer
    Authors: T Okamoto, D duVerle, K Yaginuma, Y Natsume, H Yamanaka, D Kusama, M Fukuda, M Yamamoto, F Perraudeau, U Srivastava, Y Kashima, A Suzuki, Y Kuze, Y Takahashi, M Ueno, Y Sakai, T Noda, K Tsuda, Y Suzuki, S Nagayama, R Yao
    Stem Cell Reports, 2021;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  10. Murine astrovirus tropism for goblet cells and enterocytes facilitates an IFN-&lambda response in vivo and in enteroid cultures
    Authors: H Ingle, E Hassan, J Gawron, B Mihi, Y Li, EA Kennedy, G Kalugotla, H Makimaa, S Lee, P Desai, KG McDonald, MS Diamond, RD Newberry, M Good, MT Baldridge
    Mucosal Immunology, 2021;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  11. LGR5/R-Spo1/Wnt3a axis promotes stemness and aggressive phenotype in hepatoblast-like hepatocellular carcinoma cell lines
    Authors: S Akbari, ? Kunter, Y Azbazdar, G Ozhan, N Atabey, ZF Karagonlar, E Erdal
    Cellular Signalling, 2021;0(0):109972.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Targeted Profiling of Immunological Genes during Norovirus Replication in Human Intestinal Enteroids
    Authors: JCM Chan, KN Mohammad, LY Zhang, SH Wong, MC Chan
    Viruses, 2021;13(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Measuring the elastic modulus of soft culture surfaces and three-dimensional hydrogels using atomic force microscopy
    Authors: MDA Norman, SA Ferreira, GM Jowett, L Bozec, E Gentleman
    Nature Protocols, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Intestinal antiviral signaling is controlled by autophagy gene Epg5 independent of the microbiota
    Authors: S Lee, G Kalugotla, H Ingle, R Rodgers, C Wu, Y Wang, Y Li, X Yang, J Zhang, NR Borella, H Deng, L Droit, R Hill, ST Peterson, C Desai, D Lawrence, Q Lu, MT Baldridge
    Autophagy, 2021;0(0):1-16.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  15. A beta-Catenin-TCF-Sensitive Locus Control Region Mediates GUCY2C Ligand Loss in Colorectal Cancer
    Authors: JA Rappaport, AA Entezari, A Caspi, S Caksa, AV Jhaveri, TJ Stanek, A Ertel, J Kupper, PM Fortina, SB McMahon, JB Jaynes, AE Snook, SA Waldman
    Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  16. Capturing human trophoblast development with naive pluripotent stem cells in�vitro
    Authors: S Io, M Kabata, Y Iemura, K Semi, N Morone, A Minagawa, B Wang, I Okamoto, T Nakamura, Y Kojima, C Iwatani, H Tsuchiya, B Kaswandy, E Kondoh, S Kaneko, K Woltjen, M Saitou, T Yamamoto, M Mandai, Y Takashima
    Cell Stem Cell, 2021;28(6):1023-1039.e13.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  17. Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer
    Authors: B Orr, K Sutton, S Christian, T Nash, H Niemann, LL Hansen, MJ McGrew, SR Jensen, L Vervelde
    Veterinary research, 2021;52(1):142.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas
    Authors: S Heino, S Fang, M Lähde, J Högström, S Nassiri, A Campbell, D Flanagan, A Raven, M Hodder, N Nasreddin, HH Xue, M Delorenzi, S Leedham, TV Petrova, O Sansom, K Alitalo
    Science Advances, 2021;7(47):eabj0512.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  19. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
    Authors: SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang
    Nature Communications, 2021;12(1):262.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  20. Farnesyl dimethyl chromanol targets colon cancer stem cells and prevents colorectal cancer metastasis
    Authors: K Husain, D Coppola, CS Yang, MP Malafa
    Scientific Reports, 2021;11(1):2185.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  21. A pan-cancer organoid platform for precision medicine
    Authors: BM Larsen, M Kannan, LF Langer, BD Leibowitz, A Bentaieb, A Cancino, I Dolgalev, BE Drummond, JR Dry, CS Ho, G Khullar, BA Krantz, B Mapes, KE McKinnon, J Metti, JF Perera, TA Rand, V Sanchez-Fr, JM Shaxted, MM Stein, MA Streit, YC Tan, Y Zhang, E Zhao, J Venkataram, MC Stumpe, JA Borgia, A Masood, DVT Catenacci, JV Mathews, DB Gursel, JJ Wei, TH Welling, DM Simeone, KP White, AA Khan, C Igartua, AA Salahudeen
    Cell Reports, 2021;36(4):109429.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  22. Targeting uPA-uPAR interaction to improve intestinal epithelial barrier integrity in inflammatory bowel disease
    Authors: Y Cheng, TR Hall, X Xu, I Yung, D Souza, J Zheng, F Schiele, M Hoffmann, ML Mbow, JP Garnett, J Li
    EBioMedicine, 2021;75(0):103758.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  23. Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1
    Authors: K Dunbar, A Valanciute, A Lima, PF Vinuela, T Jamieson, V Rajasekara, J Blackmur, AM Ochocka-Fo, A Guazzelli, P Cammareri, MJ Arends, OJ Sansom, KB Myant, SM Farrington, MG Dunlop, FVN Din
    Cell Mol Gastroenterol Hepatol, 2020;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Cell Culture
  24. Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells
    Authors: G Levin, BAA Koga, GG Belchior, ACO Carreira, MC Sogayar
    BMC Biotechnol., 2020;20(1):5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
    Authors: S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi
    Sci Rep, 2020;10(1):5989.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Standardized GMP-compliant scalable production of human pancreas organoids
    Authors: M Dossena, R Piras, A Cherubini, M Barilani, E Dugnani, F Salanitro, T Moreth, F Pampaloni, L Piemonti, L Lazzari
    Stem Cell Res Ther, 2020;11(1):94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Tissue Culture
  27. Patient-derived ovarian cancer organoids capture the genomic profiles of primary tumours applicable for drug sensitivity and resistance testing
    Authors: Y Nanki, T Chiyoda, A Hirasawa, A Ookubo, M Itoh, M Ueno, T Akahane, K Kameyama, W Yamagami, F Kataoka, D Aoki
    Sci Rep, 2020;10(1):12581.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  28. Expansion of Human iPSC-Derived Ureteric Bud Organoids with Repeated Branching Potential
    Authors: SI Mae, M Ryosaka, S Sakamoto, K Matsuse, A Nozaki, M Igami, R Kabai, A Watanabe, K Osafune
    Cell Rep, 2020;32(4):107963.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  29. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs
    Authors: M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie
    PLoS ONE, 2020;15(3):e0229445.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Control
  30. HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells
    Authors: Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban
    Nat. Immunol., 2020;21(3):274-286.
    Species: Human
    Sample Types: Cells
    Applications: Cell Culture
  31. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
    Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Nat. Biotechnol., 2020;0(0):.
    Species: Human
    Sample Types: cell culture
    Applications: Cell Culture
  32. LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium
    Authors: RT Zwiggelaar, HT Lindholm, M Fosslie, M Terndrup P, Y Ohta, A Díez-Sánch, M Martín-Alo, J Ostrop, M Matano, N Parmar, E Kvaløy, RR Spanjers, K Nazmi, M Rye, F Drabløs, C Arrowsmith, J Arne Dahl, KB Jensen, T Sato, MJ Oudhoff
    Science Advances, 2020;6(37):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  33. Implementing cell-free DNA of pancreatic cancer patient-derived organoids for personalized oncology
    Authors: Z Dantes, HY Yen, N Pfarr, C Winter, K Steiger, A Muckenhube, A Hennig, S Lange, T Engleitner, R Öllinger, R Maresch, F Orben, I Heid, G Kaissis, K Shi, G Topping, F Stögbauer, M Wirth, K Peschke, A Papargyrio, M Rezaee-Ogh, K Feldmann, AP Schäfer, R Ranjan, C Lubeseder-, DE Stange, T Welsch, M Martignoni, GO Ceyhan, H Friess, A Herner, L Liotta, M Treiber, G von Figura, M Abdelhafez, P Klare, C Schlag, H Algül, J Siveke, R Braren, G Weirich, W Weichert, D Saur, R Rad, RM Schmid, G Schneider, M Reichert
    JCI Insight, 2020;5(15):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Cell Culture
  34. Using systems medicine to identify a therapeutic agent with potential for repurposing in inflammatory bowel disease
    Authors: K Lloyd, S Papoutsopo, E Smith, P Stegmaier, F Bergey, L Morris, M Kittner, H England, D Spiller, MHR White, CA Duckworth, BJ Campbell, V Poroikov, VAP Martins Do, A Kel, W Muller, DM Pritchard, C Probert, MD Burkitt, SysmedIBD
    Dis Model Mech, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Telomere dysfunction activates YAP1 to drive tissue inflammation
    Authors: D Chakravart, B Hu, X Mao, A Rashid, J Li, J Li, WT Liao, EM Whitley, P Dey, P Hou, KA LaBella, A Chang, G Wang, DJ Spring, P Deng, D Zhao, X Liang, Z Lan, Y Lin, S Sarkar, C Terranova, YL Deribe, SE Blutt, P Okhuysen, J Zhang, E Vilar, OH Nielsen, A Dupont, M Younes, KR Patel, NF Shroyer, K Rai, MK Estes, YA Wang, AA Bertuch, RA DePinho
    Nat Commun, 2020;11(1):4766.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  36. Establishment and differentiation of long-term trophoblast organoid cultures from the human placenta
    Authors: MA Sheridan, RC Fernando, L Gardner, MS Hollinshea, GJ Burton, A Moffett, MY Turco
    Nat Protoc, 2020;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  37. Understanding the regulatory mechanisms of endometrial cells on activities of endometrial mesenchymal stem-like cells during menstruation
    Authors: S Xu, RWS Chan, T Li, EHY Ng, WSB Yeung
    Stem Cell Res Ther, 2020;11(1):239.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Implementing cell-free DNA of pancreatic cancer patient-derived organoids for personalized oncology
    Authors: Z Dantes, HY Yen, N Pfarr, C Winter, K Steiger, A Muckenhube, A Hennig, S Lange, T Engleitner, R Öllinger, R Maresch, F Orben, I Heid, G Kaissis, K Shi, G Topping, F Stögbauer, M Wirth, K Peschke, A Papargyrio, M Rezaee-Ogh, K Feldmann, AP Schäfer, R Ranjan, C Lubeseder-, DE Stange, T Welsch, M Martignoni, GO Ceyhan, H Friess, A Herner, L Liotta, M Treiber, G von Figura, M Abdelhafez, P Klare, C Schlag, H Algül, J Siveke, R Braren, G Weirich, W Weichert, D Saur, R Rad, RM Schmid, G Schneider, M Reichert
    JCI Insight, 2020;5(15):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  39. Comparison of Surgical and Cadaveric Intestine as a Source of Crypt Culture in Humans
    Authors: A Scott, B Olack, JD Rouch, HA Khalil, BA Kokubun, NY Lei, J Wang, S Solorzano, M Lewis, JCY Dunn, MG Stelzner, JC Niland, MG Martín
    Cell Transplant, 2020;29(0):9636897209037.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  40. The Fragile X Mental Retardation Protein regulates RIP1K and colorectal cancer resistance to necroptosis
    Authors: A Di Grazia, I Marafini, G Pedini, D Di Fusco, F Laudisi, V Dinallo, E Rosina, C Stolfi, E Franzè, P Sileri, G Sica, G Monteleone, C Bagni, I Monteleone
    Cell Mol Gastroenterol Hepatol, 2020;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  41. Endometrial Axin2+ Cells Drive Epithelial Homeostasis, Regeneration, and Cancer following Oncogenic Transformation
    Authors: SM Syed, M Kumar, A Ghosh, F Tomasetig, A Ali, RM Whan, D Alterman, PS Tanwar
    Cell Stem Cell, 2019;26(1):64-80.e13.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  42. A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion
    Authors: PG Boone, LK Rochelle, JD Ginzel, V Lubkov, WL Roberts, PJ Nicholls, C Bock, ML Flowers, RJ von Furste, BR Stripp, P Agarwal, AD Borowsky, RD Cardiff, LS Barak, MG Caron, HK Lyerly, JC Snyder
    Nat Commun, 2019;10(1):5490.
    Species: Mouse
    Sample Types: Organoids
    Applications: Cell Culture
  43. Patient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders
    Authors: K Shik Mun, K Arora, Y Huang, F Yang, S Yarlagadda, Y Ramananda, M Abu-El-Hai, JJ Palermo, BN Appakalai, JD Nathan, AP Naren
    Nat Commun, 2019;10(1):3124.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  44. NOD2 Supports Crypt Survival and Epithelial Regeneration after Radiation-Induced Injury
    Authors: C Lee, C Choi, HS Kang, SW Shin, SY Kim, HC Park, SN Hong
    Int J Mol Sci, 2019;20(17):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  45. IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model
    Authors: B Zwarycz, AD Gracz, KR Rivera, IA Williamson, LA Samsa, J Starmer, MA Daniele, L Salter-Cid, Q Zhao, ST Magness
    Cell Mol Gastroenterol Hepatol, 2019;7(1):1-17.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  46. Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling
    Authors: S Akbari, GG Sevinç, N Ersoy, O Basak, K Kaplan, K Sevinç, E Ozel, B Sengun, E Enustun, B Ozcimen, A Bagriyanik, N Arslan, TT Önder, E Erdal
    Stem Cell Reports, 2019;13(4):627-641.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  47. USP9X Deubiquitylates DVL2 to Regulate WNT Pathway Specification
    Authors: CP Nielsen, KK Jernigan, NL Diggins, DJ Webb, JA MacGurn
    Cell Rep, 2019;28(4):1074-1089.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  48. Mist1 Expression is Required for Paneth Cell Maturation
    Authors: CM Dekaney, S King, B Sheahan, J Cortes
    Cell Mol Gastroenterol Hepatol, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  49. Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue
    Authors: OC Tysoe, AW Justin, T Brevini, SE Chen, KT Mahbubani, AK Frank, H Zedira, E Melum, K Saeb-Parsy, AE Markaki, L Vallier, F Sampazioti
    Nat Protoc, 2019;14(6):1884-1925.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling
    Authors: HLX Wong, HY Qin, SW Tsang, X Zuo, S Che, CFW Chow, X Li, HT Xiao, L Zhao, T Huang, CY Lin, HY Kwan, T Yang, FM Longo, A Lyu, ZX Bian
    Nat Commun, 2019;10(1):1745.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  51. Organoid culture media formulated with growth factors of defined cellular activity
    Authors: M Urbischek, H Rannikmae, T Foets, K Ravn, M Hyvönen, M de la Roch
    Sci Rep, 2019;9(1):6193.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells
    Authors: S Konagaya, H Iwata
    Sci Rep, 2019;9(1):640.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Cell-type specific potent Wnt signaling blockade by bispecific antibody
    Authors: NK Lee, Y Zhang, Y Su, S Bidlingmai, DW Sherbenou, KD Ha, B Liu
    Sci Rep, 2018;8(1):766.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA
    Authors: YC Chen, CL Tsai, YH Wei, YT Wu, WT Hsu, HC Lin, YC Hsu
    Cell Death Dis, 2018;9(4):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. R-spondins can potentiate WNT signaling without LGRs
    Authors: AM Lebensohn, R Rohatgi
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  56. Differential activities and mechanisms of the four R-Spondins in potentiating Wnt/?-catenin signaling
    Authors: S Park, J Cui, WA Yu, L Wu, K Carmon, QJ Liu
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery
    Authors: R Cruz-Acuña, M Quirós, S Huang, D Siuda, JR Spence, A Nusrat, AJ García
    Nat Protoc, 2018;0(0):.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  58. A surgical orthotopic organoid transplantation approach in mice to visualize and study colorectal cancer progression
    Authors: A Fumagalli, SJE Suijkerbui, H Begthel, E Beerling, KC Oost, HJ Snippert, J van Rheene, J Drost
    Nat Protoc, 2018;13(2):235-247.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer
    Authors: JD Barros-Sil, DE Linn, I Steiner, G Guo, A Ali, H Pakula, G Ashton, I Peset, M Brown, NW Clarke, RT Bronson, GC Yuan, SH Orkin, Z Li, E Baena
    Cell Rep, 2018;25(12):3504-3518.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA
    Authors: MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak
    Cell Rep, 2018;25(13):3811-3827.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  61. Culture and characterization of chicken small intestinal crypts
    Authors: J Li, J Li, SY Zhang, RX Li, X Lin, YL Mi, CQ Zhang
    Poult. Sci., 2018;0(0):.
    Species: Avian - Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  62. Establishment of a refined culture method for rat colon organoids
    Authors: H Isshiki, Y Arimura, K Nagaishi, K Kawakami, K Onodera, K Yamashita, Y Naishiro, M Fujimiya, K Imai, Y Shinomura
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Id2 controls specification of Lgr5+ intestinal stem cell progenitors during gut development
    Authors: L Nigmatulli, M Norkin, MM Dzama, B Messner, S Sayols, N Soshnikova
    EMBO J., 2017;36(7):869-885.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  64. P2X7 receptor-dependent tuning of gut epithelial responses to infection
    Authors: SW Huang, C Walker, J Pennock, K Else, W Muller, MJ Daniels, C Pellegrini, D Brough, G Lopez-Cast, SM Cruickshan
    Immunol. Cell Biol, 2017;95(2):178-188.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  65. Directional delivery of RSPO1 by mesenchymal stem cells ameliorates radiation-induced intestinal injury
    Authors: W Chen, S Ju, T Lu, Y Xu, X Zheng, H Wang, Y Ge, S Ju
    Cytokine, 2017;95(0):27-34.
  66. R-Spondin1/LGR5 Activates TGF? Signaling and Suppresses Colon Cancer Metastasis
    Authors: X Zhou, L Geng, D Wang, H Yi, G Talmon, J Wang
    Cancer Res., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  67. Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor
    Authors: SH Han, S Shim, MJ Kim, HY Shin, WS Jang, SJ Lee, YW Jin, SS Lee, SB Lee, S Park
    World J. Gastroenterol, 2017;23(6):964-975.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  68. Higher-Order Kidney Organogenesis from Pluripotent Stem Cells
    Authors: A Taguchi, R Nishinakam
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  69. Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway
    Authors: Y Gao, A Cai, H Xi, J Li, W Xu, Y Zhang, K Zhang, J Cui, X Wu, B Wei, L Chen
    Stem Cell Res Ther, 2017;8(1):98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  70. Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids.
    Authors: Rouch J, Scott A, Lei N, Solorzano-Vargas R, Wang J, Hanson E, Kobayashi M, Lewis M, Stelzner M, Dunn J, Eckmann L, Martin M
    PLoS ONE, 2016;11(1):e0148216.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  71. Novel Bi-specific Domain Antibody to LRP6 Inhibits Wnt and R-spondin Ligand-induced Wnt Signaling and Tumor Growth
    Mol Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  72. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
    Sci Rep, 2016;6(0):36795.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  73. Intestinal enteroids model GUCY2C-dependent secretion induced by heat-stable enterotoxins
    Infect Immun, 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  74. Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates beta-Catenin Signaling and Tumorigenesis in Multiple Cancer Types.
    Authors: Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee-Hoskins C, Ma S, Fischer M, Shah J, Wei J, Ji M, Lam A, Stroud M, Yen W, Yeung P, Cancilla B, O'Young G, Wang M, Kapoun A, Lewicki J, Hoey T, Gurney A
    Cancer Res, 2016;76(3):713-23.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  75. Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note.
    Authors: James M, Howells L, Karmokar A, Higgins J, Greaves P, Cai H, Dennison A, Metcalfe M, Garcea G, Lloyd D, Berry D, Steward W, Brown K
    PLoS ONE, 2015;10(2):e0117776.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  76. RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.
    Authors: Carmon K, Gong X, Yi J, Thomas A, Liu Q
    Proc Natl Acad Sci U S A, 2014;111(13):E1221-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  77. Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium.
    Authors: Jabaji Z, Brinkley G, Khalil H, Sears C, Lei N, Lewis M, Stelzner M, Martin M, Dunn J
    PLoS ONE, 2014;9(9):e107814.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  78. Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.
    Authors: Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A
    J Immunol, 2014;193(1):439-51.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  79. Intestinal subepithelial myofibroblasts support the growth of intestinal epithelial stem cells.
    Authors: Lei N, Jabaji Z, Wang J, Joshi V, Brinkley G, Khalil H, Wang F, Jaroszewicz A, Pellegrini M, Li L, Lewis M, Stelzner M, Dunn J, Martin M
    PLoS ONE, 2014;9(1):e84651.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  80. Hippo pathway activity influences liver cell fate.
    Authors: Yimlamai D, Christodoulou C, Galli G, Yanger K, Pepe-Mooney B, Gurung B, Shrestha K, Cahan P, Stanger B, Camargo F
    Cell, 2014;157(6):1324-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  81. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer.
    Authors: Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X
    Nat Commun, 2014;5(0):3149.
  82. Inner ear hair cell-like cells from human embryonic stem cells.
    Authors: Ronaghi, Mohammad, Nasr, Marjan, Ealy, Megan, Durruthy-Durruthy, Robert, Waldhaus, Joerg, Diaz, Giovanni, Joubert, Lydia-Ma, Oshima, Kazuo, Heller, Stefan
    Stem Cells Dev, 2014;23(11):1275-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  83. Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway.
    Authors: Guezguez A, Pare F, Benoit Y, Basora N, Beaulieu J
    Exp Cell Res, 2014;322(2):355-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  84. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
    Authors: Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, Markmann J, Miyazaki S, Miyazaki J, Szot G, Bottino R, Kim S
    Elife, 2013;2(0):e00940.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  85. Role of Gadd45a in Wip1-dependent regulation of intestinal tumorigenesis.
    Cell Death Differ., 2012;19(11):1761-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  86. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.
    Authors: Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F
    Nature, 2012;485(7397):195-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  87. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  88. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
    Authors: Spence JR, Mayhew CN, Rankin SA
    Nature, 2011;470(7332):105-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  89. Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling.
    Authors: Lebensohn A, Dubey R, Neitzel L, Tacchelly-Benites O, Yang E, Marceau C, Davis E, Patel B, Bahrami-Nejad Z, Travaglini K, Ahmed Y, Lee E, Carette J, Rohatgi R
    Elife, 0;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human R-Spondin 1 Protein

Average Rating: 4.7 (Based on 6 Reviews)

5 Star
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Recombinant Human R-Spondin 1 Protein
By Andres Ocampo Carrillo on 03/04/2023
Application: 3D culture of cells
Reason for Rating: R-spondin was used as indicated by the manufacturer and stored in aliquots. The product was easy to dissolve and allowed us replicable and excellent results in 3D culture

R-Spondin was used for colonocyte isolation and culturing from Sprague-Dawley rats in 3D culture. Results showed a purified culture as spheroids-like, following a standard isolation protocol for these cells

Other Recombinant Human R-Spondin 1 Protein 4645-RS
Recombinant Human R-Spondin 1 Protein
By Anonymous on 09/26/2021
Application: Stem/Immune cell maintenance or differentiation

Used for the differentiation of iPSC into intestinal organoids, at a concentration of 500ng/mL

Recombinant Human R-Spondin 1 Protein
By Ermali Aliaj on 01/02/2021
Application: Cell Culture
Recombinant Human R-Spondin 1 Protein
By Anonymous on 07/24/2020
Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: stable product when used as described; produces repeatable results for differentiation
Recombinant Human R-Spondin 1 Protein
By Anonymous on 03/06/2020
Application: In vitro bioactivity in cell culture
By Anonymous on 08/15/2017
Application: In vitro bioactivity in cell culture
Reason for Rating: Protein performed as expected in assay

Wnt3a biology and cell growth. Use HEK Wnt3a reporter cell line.