Recombinant Human R-Spondin 1 Protein

(24 citations)
(1 Review)
  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1-4 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Arg31-Ala263
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg31
  • Predicted Molecular Mass
    25.6 kDa
  • SDS-PAGE
    39 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4645-RS
 
4645-RS/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human R-Spondin 1 (Catalog # 4645-RS), in the presence of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN; 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay (blue). The activity is approximately 7-fold greater than the competitor's R-Spondin 1 (red).
1 µg/lane of Recombinant Human
R-Spondin 1 was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 39 kDa.
Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three otherR-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta -catenin signaling, but have distinct expression patterns (1-3). Like other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential
N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta -catenin stabilization (2, 4). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (5). Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missingaa 146-208, respectively (6). Over aa 21-263, human R-Spondin 1 shares 89%, 87%, 92%, 91%, 91% and 89% aa identity with mouse, rat, equine, canine, caprine and bovine RSPO-1, respectively. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 5). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Disruption is also associated with palmoplantar keratosis (7, 8). Postnatally,R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta -catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). R-Spondin 1 regulates Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (11). Reports differ on whether R-Spondin 1 binds LRP-6 directly (11-13).

  • References:
    1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
    2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
    3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
    4. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
    5. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
    6. Entrez accession # EAX07331, ABA54598.
    7. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
    8. Parma, P. et al. (2006) Nat. Genet. 38:1304.
    9. Kim, K.-A. et al. (2005) Science 309:1256.
    10. Zhao, J. et al. (2007) Gastroenterology 132:1331.
    11. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
    12. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
    13. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  • Long Name:
    Roof Plate-specific Spondin 1
  • Entrez Gene IDs:
    284654 (Human); 192199 (Mouse)
  • Alternate Names:
    Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; hRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. R-Spondin1/LGR5 Activates TGF? Signaling and Suppresses Colon Cancer Metastasis
    Authors: X Zhou, L Geng, D Wang, H Yi, G Talmon, J Wang
    Cancer Res., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Directional delivery of RSPO1 by mesenchymal stem cells ameliorates radiation-induced intestinal injury
    Authors: W Chen, S Ju, T Lu, Y Xu, X Zheng, H Wang, Y Ge, S Ju
    Cytokine, 2017;95(0):27-34.
  3. Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway
    Authors: Y Gao, A Cai, H Xi, J Li, W Xu, Y Zhang, K Zhang, J Cui, X Wu, B Wei, L Chen
    Stem Cell Res Ther, 2017;8(1):98.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor
    Authors: SH Han, S Shim, MJ Kim, HY Shin, WS Jang, SJ Lee, YW Jin, SS Lee, SB Lee, S Park
    World J. Gastroenterol, 2017;23(6):964-975.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. P2X7 receptor-dependent tuning of gut epithelial responses to infection
    Authors: SW Huang, C Walker, J Pennock, K Else, W Muller, MJ Daniels, C Pellegrini, D Brough, G Lopez-Cast, SM Cruickshan
    Immunol. Cell Biol, 2017;95(2):178-188.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  6. Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates beta-Catenin Signaling and Tumorigenesis in Multiple Cancer Types.
    Authors: Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee-Hoskins C, Ma S, Fischer M, Shah J, Wei J, Ji M, Lam A, Stroud M, Yen W, Yeung P, Cancilla B, O'Young G, Wang M, Kapoun A, Lewicki J, Hoey T, Gurney A
    Cancer Res, 2016;76(3):713-23.
    Species: Mouse
    Sample Type: In Vivo
    Application: Bioassay
  7. Intestinal enteroids model GUCY2C-dependent secretion induced by heat-stable enterotoxins
    Infect Immun, 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  8. Novel Bi-specific Domain Antibody to LRP6 Inhibits Wnt and R-spondin Ligand-induced Wnt Signaling and Tumor Growth
    Mol Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
    Sci Rep, 2016;6(0):36795.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids.
    Authors: Rouch J, Scott A, Lei N, Solorzano-Vargas R, Wang J, Hanson E, Kobayashi M, Lewis M, Stelzner M, Dunn J, Eckmann L, Martin M
    PLoS ONE, 2016;11(1):e0148216.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note.
    Authors: James M, Howells L, Karmokar A, Higgins J, Greaves P, Cai H, Dennison A, Metcalfe M, Garcea G, Lloyd D, Berry D, Steward W, Brown K
    PLoS ONE, 2015;10(2):e0117776.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.
    Authors: Carmon K, Gong X, Yi J, Thomas A, Liu Q
    Proc Natl Acad Sci U S A, 2014;111(13):E1221-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Hippo pathway activity influences liver cell fate.
    Authors: Yimlamai D, Christodoulou C, Galli G, Yanger K, Pepe-Mooney B, Gurung B, Shrestha K, Cahan P, Stanger B, Camargo F
    Cell, 2014;157(6):1324-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  14. Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.
    Authors: Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A
    J Immunol, 2014;193(1):439-51.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  15. Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway.
    Authors: Guezguez A, Pare F, Benoit Y, Basora N, Beaulieu J
    Exp Cell Res, 2014;322(2):355-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Intestinal subepithelial myofibroblasts support the growth of intestinal epithelial stem cells.
    Authors: Lei N, Jabaji Z, Wang J, Joshi V, Brinkley G, Khalil H, Wang F, Jaroszewicz A, Pellegrini M, Li L, Lewis M, Stelzner M, Dunn J, Martin M
    PLoS ONE, 2014;9(1):e84651.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  17. Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium.
    Authors: Jabaji Z, Brinkley G, Khalil H, Sears C, Lei N, Lewis M, Stelzner M, Martin M, Dunn J
    PLoS ONE, 2014;9(9):e107814.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer.
    Authors: Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X
    Nat Commun, 2014;5(0):3149.
  19. Inner ear hair cell-like cells from human embryonic stem cells.
    Authors: Ronaghi, Mohammad, Nasr, Marjan, Ealy, Megan, Durruthy-Durruthy, Robert, Waldhaus, Joerg, Diaz, Giovanni, Joubert, Lydia-Ma, Oshima, Kazuo, Heller, Stefan
    Stem Cells Dev, 2014;23(11):1275-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.
    Authors: Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, Markmann J, Miyazaki S, Miyazaki J, Szot G, Bottino R, Kim S
    Elife, 2013;2(0):e00940.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Role of Gadd45a in Wip1-dependent regulation of intestinal tumorigenesis.
    Cell Death Differ., 2012;19(11):1761-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.
    Authors: Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F
    Nature, 2012;485(7397):195-200.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
    Authors: Spence JR, Mayhew CN, Rankin SA
    Nature, 2011;470(7332):105-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 24 Citations
Average Rating: 4 (Based on 1 review)

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Summary

ApplicationIn vitro bioactivity in cell culture
Reason for RatingProtein performed as expected in assay

Other Experimental Details

Other Experimental DetailsWnt3a biology and cell growth. Use HEK Wnt3a reporter cell line.

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