Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein, CF

Name: Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI11508
Price: 536 USD
Availability: InStock

R&D Systems | Catalog # AVI11508

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Product Documents

Fc Chimera, biotinylated

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein (AVI11508)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P19320.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all VCAM-1/CD106 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VCAM-1/CD106 protein

Human VCAM-1 (Phe25-Glu698) Accession # P19320.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe25

Predicted Molecular Mass

103 kDa

SDS-PAGE

105-130 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human VCAM 1/CD106 Fc Chimera Avi-tag (Catalog # AVI11508) binds Recombinant Human Integrin alpha 4 beta 1 (Catalog # 5668-A4) with an ED₅₀ of 7.50-90.0 ng/mL.

Scientific Data Images for Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein Binding Activity.

Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein (Catalog # AVI11508) binds Recombinant Human Integrin alpha 4 beta 1 (5668-A4) with an ED₅₀ of 7.50‑90.0 ng/mL.

Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein (Catalog # AVI11508) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-130 kDa, and 210-260 kDa, respectively.

Formulation, Preparation, and Storage

AVI11508

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VCAM-1/CD106

Human VCAM-1 (CD106, INCAM-110) is a cell adhesion molecule and a member of the immunoglobulin superfamily. Alternatively spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 amino acid extracellular segment that includes seven C2-type immunoglobulin domains. VCAM-1 is expressed by activated endothelial cells and certain other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions. Our Avi-tag Biotinylated human VCAM-1 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.