Recombinant Human VISTA/B7-H5/PD-1H Fc Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI7126-050
Recombinant Human VISTA/B7-H5/PD-1H  Fc Avi-tag Protein, CF Binding Activity
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human VISTA/B7-H5/PD-1H Fc Avi-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) is immobilized at 5 µg/mL (100 µL/well), Biotinylated Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Avi-tag (Catalog # AVI7126) binds with an ED50 of 0.5-3 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human VISTA/B7-H5/PD-1H protein
Human VISTA/B7-H5/PD-1H
(Phe33-Ala194)
Accession # AAH20568
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Phe33
Structure / Form
Disulfide-linked homodimer, biotinylated protein via Avi-tag
Predicted Molecular Mass
47 kDa
SDS-PAGE
64-75 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI7126

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity View Larger

When Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) is coated at 5 µg/mL, Biotinylated Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Avi-tag (Catalog # AVI7126) binds with an ED50of 0.5-3 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Avi-tag (Catalog # AVI7126) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-75 kDa and 130-150 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: VISTA/B7-H5/PD-1H

Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7‑H5, is a 55‑65 kDa transmembrane glycoprotein with homology to B7‑like immune co‑stimulatory molecules (1, 2). Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V‑type Ig‑like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively (3). The 30 kDa ECD can be shed by MT1‑MMP, with a 25‑30 kDa fragment remaining in the membrane (3). Gi24 promotes both MT1‑MMP expression and the MT1‑MMP mediated activation of MMP‑2 (3). Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP‑4 induced signaling in ESC, but is also down‑regulated following BMP‑4 exposure (4, 5). It binds to BMP‑4 directly, and also associates with the type I BMP receptor Activin RIB/ALK‑4 (4, 5). Gi24 is expressed on the surface of naïve CD4+ T cells and regulatory T cells (6). It is up‑regulated in vivo on activated monocytes and dendritic cells (5). Gi24 inhibits CD4+ and CD8+ T cell proliferation, and their production of IL‑2 and IFN‑ gamma (6). Its expression on tumor cells attenuates the anti‑tumor immune response and enables more rapid tumor progression (6). In contrast, Gi24 limits disease progression in the autoimmune disease model EAE (6).

References
  1. Flajnik, M.F. et al. (2012) Immunogenetics 64:571.
  2. Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
  3. Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
  4. Aloia, L. et al. (2010) J. Biol. Chem. 285:7776.
  5. Parisi, S. et al. (2012) FASEB J. 26:3957.
  6. Wang, L. et al. (2011) J. Exp. Med. 208:577.
Entrez Gene IDs
64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)
Alternate Names
4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human VISTA/B7-H5/PD-1H Fc Avi-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human VISTA/B7-H5/PD-1H Fc Avi-tag Protein, CF and earn rewards!

Have you used Recombinant Human VISTA/B7-H5/PD-1H Fc Avi-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review