Recombinant Mouse CD69 Protein, CF

R&D Systems | Catalog # 8469-CD

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CD69 Protein (8469-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P37217

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD69 protein
Gly64-Arg199 with an N-terminal 9-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

17 kDa

SDS-PAGE

25-37 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD69 is coated at  1 µg/mL (100 µL/well), the concentration of Recombinant Mouse Galectin-1 (Catalog # 1245‑GA) that produces a 50% optimal binding response is typically 15-90 ng/mL.

Scientific Data Images for Recombinant Mouse CD69 Protein, CF

Recombinant Mouse CD69 Protein Binding Activity

Recombinant Mouse CD69 Protein Binding Activity

When Recombinant Mouse CD69 (Catalog # 8469-CD) is coated at 1 µg/mL (100 µL/well), Recombinant Mouse Galectin-1 (Catalog # 1245-GA) binds with an ED50 of 15-90 ng/mL.

Recombinant Mouse CD69 Protein SDS-PAGE

Recombinant Mouse CD69 Protein SDS-PAGE

1 μg/lane of Recombinant Mouse CD69 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 25 - 37 kDa and NR bands at 55-60 kDa.

Formulation, Preparation, and Storage

8469-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at&nbsp;250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD69

CD69, also known as CLEC2C, is a type 2 transmembrane glycoprotein in the C-type lectin family. It plays roles in immune cell trafficking, inflammation, T cell memory, and humoral immune responses (1). Mature mouse CD69 consists of a 40 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 138 aa extracellular domain with one C-type lectin domain (CTL) (3). Within the ECD, mouse CD69 shares 57% and 76% aa sequence identity with human and rat CD69, respectively. CD69 is expressed on the cell surface as an approximately 60 kDa disulfide-linked homodimer (3-5). It is found on CD4+ T cells, CD8+ T cells, NK cells, NKT cells, gamma delta cells dendritic cells (DC) and is up-regulated on activated T cells and DC (6-8). Ligation of CD69 on DC induces IL-2 production, leading to T cell proliferation (6). CD69 is important for the homing of CD4+ T cells and plasmablasts to the bone marrow but inhibits the migration of dermal DC to draining lymph nodes (7, 9). It supports the expression of multiple chemokines and chemokine receptors but suppresses the expression of others (10, 11). It associates with and negatively regulates S1P1 expression on DC and CD4+ T cells, resulting in a decreased chemotactic response to S1P (7, 12, 13). S1P1 similarly inhibits the cell surface expression of CD69 (12). The direct interaction of CD69 with Galectin-1 contributes to the ability of CD69 to limit Th17 mediated inflamamtion while supporting the differentiation of regulatory T cells (8, 10, 13-16). In various disease models, CD69 has been shown to have both enhancing and inhibitory effects on Th17 and Th2 cell mediated inflammation (8, 10, 11, 13, 16, 17).

References

  1. Gonzalez-Amaro, R. et al. (2013) Trends Mol. Med. 19:625.
  2. Ziegler, S.F. et al. (1993) Eur. J. Immunol. 23:1643.
  3. Hamann, J. et al. (1993) J. Immunol. 150:4920.
  4. Lopez-Cabrera, M. et al. (1993) J. Exp. Med. 178:537.
  5. Bieber, T. et al. (1992) J. Invest. Dermatol. 98:771.
  6. Alari-Pahissa, E. et al. (2012) J. Leukoc. Biol. 92:145.
  7. Lamana, A. et al. (2011) J. Invest. Dermatol. 131:1503.
  8. Radulovic, K. et al. (2012) J. Immunol. 188:2001.
  9. Shinoda, K. et al. (2012) Proc. Natl. Acad. Sci. USA 109:7409.
  10. Radulovic, K. et al. (2013) PLoS ONE 8:e65413.
  11. Hasegawa, A. et al. (2013) PLoS ONE 8:e65494.
  12. Shiow, L.R. et al. (2006) Nature 440:540.
  13. Martin, P. et al. (2010) J. Allergy Clin. Immunol. 126:355.
  14. Martin, P. et al. (2010) Mol. Cell. Biol. 30:4877.
  15. de la Fuente, H. et al. (2014) Mol. Cell. Biol. 34:2479.
  16. Cruz-Adalia, A. et al. (2010) Circulation 122:1396.
  17. Miki-Hosokawa, T. et al. (2009) J. Immunol. 183:8203.

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Gene Symbol

CD69

UniProt

P37217

Additional CD69 Products

Product Documents for Recombinant Mouse CD69 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD69 Protein, CF

For research use only

Citations for Recombinant Mouse CD69 Protein, CF

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