Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9364-EP-100
Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein Bioactivity
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Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. When 5 x 104 cells/well are added to Recombinant Mouse EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.4-2.4 μg/mL
Source
Mouse myeloma cell line, NS0-derived mouse EpCAM/TROP1 protein
Mouse EpCAM/TROP1
(Gln24 & Arg81-Thr266)
Accession # Q99JW5
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Arg81 detected. Gln24 inferred from enzymatic pyroglutamate treatment revealing Arg25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
55 kDa
SDS-PAGE
58-75 kDa and 8 kDa

Product Datasheets

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9364-EP

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9364-EP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein Bioactivity View Larger

Immobilized Recombinant Mouse EpCAM/TROP1 (Catalog # 9364-EP) enhances L Cells mouse fibroblasts adhesion in the presence of Human Fibronectin (Catalog # 1918-FN). The ED50 for this effect is 0.4-2.4 µg/mL.

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Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, CD326 and TROP‑1, is a 40 kDa transmembrane glycoprotein composed of a 243 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF‑like) repeats, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In mouse, EpCAM also shares 51% aa sequence homology with Trop‑2. EpCAM is detected in basolateral cell membranes of all simple epithelia and expression has been found to increase in epithelia tissues during fetal development (1, 2). EpCAM has been shown to function as a homophilic Ca2+ independent adhesion molecule, but it does not structurally resemble any of the four major families of cell adhesion molecules (3). Homophilic adhesion via EpCAM requires the interaction of both EGF‑like repeats, with the first EGF‑like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM (4). EpCAM has been identified as a surface marker for pluripotent embryonic stem cells and is strongly associated with the maintenance of the undifferentiated state of ESCs (5). EpCAM is highly expressed on the surface of epithelial cancer cells including colon, stomach, lung, pancreas, prostate and breast (6, 7).

References
  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Keisuke, N. et al. (2009) PLoS One 4 :e8543
  3. Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. González, B. et al. (2009) Stem Cells, 27: 1782.
  6. Went, P. et al. (2006) Br. J. Cancer 94:128.
  7. Spizzo, G.S. et al. (2002) Int. J. Cancer 98:883.
Long Name
Epithelial Cell Adhesion Molecule
Entrez Gene IDs
4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

FAQs

  1. Can you please provide the sequence of the Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF (Catalog # 9364-EP) and confirm that there is only one band in SDS-PAGE?

    • The sequence information of Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF (Catalog # 9364-EP) can be found under the Source section of the product datasheet. It is an NS0-derived mouse EpCAM/TROP1 protein with Gln24 & Arg81-Thr266 amino acid sequence that is fused to Mouse IgG2a via a linker sequence. Under reducing conditions in SDS-PAGE the protein migrates as a broad band at ~58-75 kDa and an 8 kDa band as well. More information can be requested by contacting techsupport@bio-techne.com.

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