Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

R&D Systems | Catalog # 9364-EP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein (9364-EP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q99JW5

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse EpCAM/TROP1 protein
Mouse EpCAM/TROP1
(Gln24 & Arg81-Thr266)
Accession # Q99JW5		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg81 detected. Gln24 inferred from enzymatic pyroglutamate treatment revealing Arg25

Predicted Molecular Mass

55 kDa

SDS-PAGE

58-75 kDa and 8 kDa

Activity

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.
When 5 x 104 cells/well are added to Recombinant Mouse EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.4-2.4 μg/mL

Scientific Data Images for Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein Bioactivity

Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein Bioactivity

Immobilized Recombinant Mouse EpCAM/TROP1 (Catalog # 9364-EP) enhances L Cells mouse fibroblasts adhesion in the presence of Human Fibronectin (Catalog # 1918-FN). The ED50 for this effect is 0.4-2.4 µg/mL.

Formulation, Preparation, and Storage

9364-EP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, CD326 and TROP‑1, is a 40 kDa transmembrane glycoprotein composed of a 243 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF‑like) repeats, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In mouse, EpCAM also shares 51% aa sequence homology with Trop‑2. EpCAM is detected in basolateral cell membranes of all simple epithelia and expression has been found to increase in epithelia tissues during fetal development (1, 2). EpCAM has been shown to function as a homophilic Ca2+ independent adhesion molecule, but it does not structurally resemble any of the four major families of cell adhesion molecules (3). Homophilic adhesion via EpCAM requires the interaction of both EGF‑like repeats, with the first EGF‑like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM (4). EpCAM has been identified as a surface marker for pluripotent embryonic stem cells and is strongly associated with the maintenance of the undifferentiated state of ESCs (5). EpCAM is highly expressed on the surface of epithelial cancer cells including colon, stomach, lung, pancreas, prostate and breast (6, 7).

References

  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Keisuke, N. et al. (2009) PLoS One 4 :e8543
  3. Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. González, B. et al. (2009) Stem Cells, 27: 1782.
  6. Went, P. et al. (2006) Br. J. Cancer 94:128.
  7. Spizzo, G.S. et al. (2002) Int. J. Cancer 98:883.

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Gene Symbol

EPCAM

UniProt

Q99JW5

Additional EpCAM/TROP1 Products

Product Documents for Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

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FAQs for Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

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  • Q: Can you please provide the sequence of the Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF (Catalog # 9364-EP) and confirm that there is only one band in SDS-PAGE?

    A: The sequence information of Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF (Catalog # 9364-EP) can be found under the Source section of the product datasheet. It is an NS0-derived mouse EpCAM/TROP1 protein with Gln24 & Arg81-Thr266 amino acid sequence that is fused to Mouse IgG2a via a linker sequence. Under reducing conditions in SDS-PAGE the protein migrates as a broad band at ~58-75 kDa and an 8 kDa band as well. More information can be requested by contacting techsupport@bio-techne.com.

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