Recombinant Mouse EphA4 Fc Chimera Protein, CF

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Recombinant Mouse EphA4 Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphA4 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant human Ephrin-A5 Fc Chimera with a linear range of 0.16-10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived mouse EphA4 protein
Mouse EphA4
Accession # Q03137
6 His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.7 kDa (monomer)
110 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, CHAPS, Imidazole and PEG 3350.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: EphA4

EphA4, also known as Hek8, Tyro1, and Sek, is a 120‑130 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases (1, 2). The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA4 is unusual in its ability to be activated by both Ephrin-A and -B molecules, although its interactions with Ephrin-B2 and -B3 are weaker than with Ephrin-A ligands (3, 4). Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. The 528 amino acid (aa) extracellular domain (ECD) of mouse EphA4 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type III domains (FnIII). The 417 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (5). Within the ECD, mouse EphA4 shares 98.3% and 99.6% aa sequence identity with human and rat EphA4, respectively. Alternate splicing of mouse EphA4 generates a short isoform with a 50 aa deletion within the kinase domain. EphA4 is activated by interactions with Ephrin ligands, triggering a repulsive effect on neurite migration (6, 7). This function is important for the accurate guidance and pathfinding of neurites and axons in the spiral ganglion of the cochlea, the anterior commissure, and the corticospinal tract (6‑8). Neuronal EphA4 interactions with astrocyte‑expressed Ephrins also plays a critical role in long term potentiation by regulating hippocampal neuron dendrite arborization, spine maturation, and function (9‑11). The up‑regulation of EphA4 in gastric carcinoma is negatively correlated with patient survival (12). In glioma, EphA4 associates with FGF R1, and this enhances FGF basic-induced tumor cell migration (13). EphA4 is also involved in morphogenesis of the thymic epithelium and T cell development (14).

  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Gale, N.W. et al. (1996) Neuron 17:9.
  4. Qin, H. et al. (2010) J. Biol. Chem. 285:644.
  5. Gilardi-Hebenstreit, P. et al. (1992) Oncogene 7:2499.
  6. Brors, D. et al. (2003) J. Comp. Neurol. 462:90.
  7. Ho, S.K.Y. et al. (2009) Neuroscience 160:784.
  8. Canty, A.J. et al. (2006) Proc. Natl. Acad. Sci. 103:15629.
  9. Murai, K.K. et al. (2003) Nat. Neurosci. 6:153.
  10. Filosa, A. et al. (2009) Nat. Neurosci. 12:1285.
  11. Fu, A.K.Y. et al. (2011) Nat. Neurosci. 14:181.
  12. Oki, M. et al. (2008) World J. Gastroenterol. 14:5650.
  13. Fukai, J. et al. (2008) Mol. Cancer Ther. 7:2768.
  14. Munoz, J.J. et al. (2006) J. Immunol. 177:804.
Long Name
Eph Receptor A4
Entrez Gene IDs
2043 (Human); 13838 (Mouse)
Alternate Names
Cek8; EC 2.7.10; EC; EK8; EPH receptor A4; EphA4; EPH-like kinase 8; ephrin type-A receptor 4; Hek8; receptor protein-tyrosine kinase HEK8; SEK; Sek1; TYRO1 protein tyrosine kinase; Tyro1; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase TYRO1

Citations for Recombinant Mouse EphA4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Efnb2 haploinsufficiency induces early gap junction plaque disassembly and endocytosis in the cochlea
    Authors: J Defourny, C Audouard, A Davy, M Thiry
    Brain research bulletin, 2021-06-15;174(0):153-160.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Identification of new EphA4 inhibitors by virtual screening of FDA-approved drugs
    Authors: S Gu, WY Fu, AKY Fu, EPS Tong, FCF Ip, X Huang, NY Ip
    Sci Rep, 2018-05-09;8(1):7377.
    Applications: ELISA (Capture)
  3. Identification and characterization of Nanobodies targeting the EphA4 receptor
    Authors: L Schoonaert, L Rué, B Roucourt, M Timmers, S Little, L Chávez-Gut, M Dewilde, P Joyce, A Curnock, P Weber, J Haustraete, G Hassanzade, B De Stroope, L Van Den Bo, P Van Damme, R Lemmens, W Robberecht
    J. Biol. Chem., 2017-05-19;292(27):11452-11465.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Nephric duct insertion requires EphA4/EphA7 signaling from the pericloacal mesenchyme.
    Authors: Weiss A, Airik R, Bohnenpoll T, Greulich F, Foik A, Trowe M, Rudat C, Costantini F, Adams R, Kispert A
    Development, 2014-09-01;141(17):3420-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  5. EphA4 receptor is a novel negative regulator of osteoclast activity.
    Authors: Stiffel V, Amoui M, Sheng M, Mohan S, Lau K
    J Bone Miner Res, 2014-04-01;29(4):804-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. CTLA-4 is expressed by activated mouse NK cells and inhibits NK Cell IFN-gamma production in response to mature dendritic cells.
    Authors: Stojanovic A, Fiegler N, Brunner-Weinzierl M, Cerwenka A
    J Immunol, 2014-03-31;192(9):4184-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Discovery and characterization of a novel cyclic peptide that effectively inhibits ephrin binding to the EphA4 receptor and displays anti-angiogenesis activity.
    Authors: Han, Xiaofeng, Xu, Yan, Yang, Yilei, Xi, Jingle, Tian, Wang, Duggineni, Srinivas, Huang, Ziwei, An, Jing
    PLoS ONE, 2013-11-12;8(11):e80183.
    Species: Human
    Sample Types: Protein
    Applications: Adhesion Assay
  8. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
    Authors: Afshari FT, Kwok JC, Fawcett JW
    J. Neurosci., 2010-03-24;30(12):4246-55.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Temporal regulation of EphA4 in astroglia during murine retinal and optic nerve development.
    Authors: Petros TJ, Williams SE, Mason CA
    Mol. Cell. Neurosci., 2006-03-30;32(1):49-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Targeting the EphA4 receptor in the nervous system with biologically active peptides.
    Authors: Murai KK, Nguyen LN, Koolpe M, McLennan R, Krull CE, Pasquale EB
    Mol. Cell. Neurosci., 2003-12-01;24(4):1000-11.
    Species: Mouse
    Sample Types: Peptide, Whole Tissue
    Applications: Binding Assay, Bioassay
  11. Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling.
    Authors: Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB
    Nat. Neurosci., 2003-02-01;6(2):153-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  12. EphA7 Expression Identifies a Unique Neuronal Compartment in the Rat Striatum
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot Control


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