Recombinant Mouse FGFR2 alpha (IIIb) Fc Chimera Protein, CF

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5496-MF-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse FGFR2 alpha (IIIb) Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

200 ng/mL of biotinylated rmFGF1 is mixed with serially diluted rmFGF R2 alpha (IIIb)/Fc. Following incubation, the FGF R2 alpha (IIIb)/Fc-FGF1/biotin complex is captured on an EvenCoatTM Streptavidin Microplate (Catalog # CP003). Bound FGF R2 alpha (IIIb)/Fc is measured using HRP labeled Gt x Human IgG Fc. The concentration of rmFGF R2 alpha (IIIb)/Fc Chimera that produces 50% of the optimal binding was found to be approximately 150‑600 ng/mL.

Source
Mouse myeloma cell line, NS0-derived mouse FGF R2 alpha protein
Mouse FGF R2a (IIIb)
(Arg22 - Glu378) (Asn314His)
Accession # ABL89196
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Arg22, Ser24, and Leu27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
66.2 kDa (monomer)
SDS-PAGE
105-120 kDa, reducing conditions

Product Datasheets

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5496-MF

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5496-MF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS. 
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: FGFR2 alpha

Fibroblast growth factor receptor 2 alpha (FGF R2 alpha ) is a 140 kDa type I transmembrane receptor tyrosine kinase that mediates the heparan sulfate proteoglycan-dependent biological activity of several FGFs. It is a member of the FGF R family of proteins whose extracellular domains (ECD) contain three immunoglobulin (Ig)-like domains with a series of acidic residues between the first and second Ig-like domains (1, 2). Multiple forms of FGF R2 are generated by alternative splicing (1, 2). Alpha isoforms contain all three Ig-like domains, while beta forms lack the first Ig-like domain (3, 4). The C-terminal portion of the third Ig-like domain can be encoded by either of two mutually exclusive exons, giving rise to the IIIb/KGFR and IIIc/BEK isoforms with distinct ligand selectivity (3, 4). This recombinant protein corresponds to the ECD from Accession # ABL89196. It shares 94% amino acid sequence identity with the ECD of human FGF R2 alpha (IIIb). FGF R2 alpha (IIIb) signals in response to FGF-1, -3, -7, -10, and -22, leading to receptor dimerization and autophosphorylation of its tyrosine kinase domain (1, 2, 5, 6). In contrast, the IIIc isoform preferentially responds to FGF-1, -2, -4, -5, -6, -8, -9, -16, -17, -18, and -20 (5, 6). FGF R2 alpha (IIIb) is widely expressed on epithelial cells, while its ligands are typically expressed by neighboring mesenchymal cells (2, 7). Its involvement in epithelial-mesenchymal interactions plays an important role in the morphogenesis of many tissues. Knockout mice exhibit widespread developmental defects due to an unresponsiveness to normal mitogenic stimulation (8 - 11). Mutations in FGF R2 alpha (IIIb) as well as altered splicing patterns (to isoform IIIc) are associated with a variety of skin and skeletal disorders and the progression of many epithelial cancers (12 - 14).

References
  1. Wiedlocha, A. and V. Sorensen (2004) Curr. Topics Microbiol. Immunol. 286:45.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Miki, T. et al. (1991) Science 251:72.
  4. Mansukhani, A. et al. (1992) Proc. Natl. Acad. Sci. 89:3305.
  5. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  6. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  7. Finch, P.W. et al. (1995) Dev. Dyn. 203:223.
  8. Petiot, A. et al. (2003) Development 130:5493.
  9. Marguerie, A. et al. (2006) Cardiovasc. Res. 71:50.
  10. Revest, J.-M. et al. (2001) Dev. Biol. 231:47.
  11. Petiot, A. et al. (2005) Development 132:2441.
  12. Katoh, M. (2009) J. Invest. Dermatol. 129:1861.
  13. Su, N. et al. (2008) Front. Biosci. 13:2842.
  14. Acevedo, V.D. et al. (2009) Cell Cycle 8:580.
Long Name
Fibroblast Growth Factor Receptor 2 alpha
Entrez Gene IDs
2263 (Human); 14183 (Mouse)
Alternate Names
BBDS;BEK;BFR-1;CD332;CEK3;CFD1;ECT1;Fibroblast growth factor receptor;Fibroblast growth factor receptor 2;JWS;KGFR;K-SAM;Receptor protein-tyrosine kinase;TK14;TK25; FGF R2a; FGFR2 alpha

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