Recombinant Mouse GDF-15 Protein, CF

Catalog # Availability Size / Price Qty
8944-GD-025
Recombinant Mouse GDF-15 Protein, CF Bioactivity
2 Images
Product Details
FAQs
Supplemental Products
Reviews (1)

Recombinant Mouse GDF-15 Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse GSF-15 is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse Activin RIB/ALK-4 Fc Chimera (Catalog # 1477-AR) that produces 50% of the optimal binding response is approximately 1-6 μg/mL.
Source
E. coli-derived mouse GDF-15 protein
Ser189-Ala303
Accession #
N-terminal Sequence
Analysis
Ser189
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13 kDa
SDS-PAGE
9-13 kDa, reducing conditions

Product Datasheets

8944-GD

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Mouse GDF-15 Protein, CF Bioactivity View Larger

When Recombinant Mouse GDF-15 (Catalog # 8944-GD) is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse Activin RIB/ALK-4 Fc Chimera (Catalog # 1477-AR) binds with a typical ED50 of 1-6 μg/mL.

SDS-PAGE Recombinant Mouse GDF-15 Protein, CF SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse GDF-15 (Catalog # 8944-GD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 10.5 kDa and 22 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GDF-15

Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1), Placental Transforming Growth Factor  beta, Prostate-derived Factor, and Placental Bone Morphogenetic Protein, is a divergent member of the TGF-beta superfamily. Cellular responses to TGF-beta proteins are mediated by hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1-3). GDF-15 is highly expressed in placenta and brain, and it is expressed at lower levels in kidney, pancreas, prostate, and colon. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RxxR) to release the mature protein. Mature mouse GDF-15 shares 66% and 97% amino acid sequence identity with the human and rat proteins, respectively. The C-terminal domain of GDF-15 contains seven characteristic conserved cysteine residues necessary for the formation of the cysteine knot and the single inter-chain disulfide bond (4, 5). Biologically active GDF-15 is a disulfide-linked homodimer of the mature protein. GDF-15 has been shown to have various functions, including inhibition of TNF-alpha production from lipopolysaccharide-stimulated macrophages and the induction of cartilage formation (2, 6). GDF-15 also promotes neuronal survival, and hypothalamic expression of GDF-15 causes appetite suppression via modulation of neuropeptide Y and pro-opiomelanocortin levels (7-10). GDF-15 is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, promoting recovery following myocardial infarction, and regulating angiogenesis (11-15). Exposure of cardiomyocytes to GDF-15 results in Smad2 and Smad3 phosphorylation (16).

References
  1. Unsicker, K. et al. (2013) Cytokine Growth Factor Rev. 24:373.
  2. Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
  3. Fairlie, W.D. et al. (1999) J. Leukoc. Biol. 65:2.
  4. Fairlie, W.D. et al. (2001) J. Biol. Chem. 276:16911.
  5. Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
  6. Paralkar, V.M. et al. (1998) J. Biol. Chem. 273:13760.
  7. Johnen, H. et al. (2007) Nat. Med. 13:1333.
  8. Strelau, J. et al. (2000) J. Neurosci. 20:8597.
  9. Breit, S.N. et al. (2011) Growth Factors 29:187.
  10. Strelau, J. et al. (2009) J. Neurosci. 29:13640.
  11. Whitson, R.J. et al. (2013) J. Cell. Biochem. 114:1424.
  12. Rossaint, J. et al. (2013) J. Thromb. Haemost. 11:335.
  13. Song, H. et al. (2012) Mol. Biol. Rep. 39:4017.
  14. Preusch, M.R. et al. (2013) Eur. J. Med. Res. 18:19.
  15. Kempf, T. et al. (2011) Nat. Med. 17:581.
  16. Xu, J. et al. (2006) Circ. Res. 98:342.
Long Name
Growth Differentiation Factor 15
Entrez Gene IDs
9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names
GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse GDF-15 Protein, CF

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Recombinant Mouse GDF-15 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Mouse GDF-15 Protein, CF
By Anonymous on 01/27/2017