Recombinant Mouse His6-USP18 Protein, CF
Recombinant Mouse His6-USP18 Protein, CF Summary
Product Specifications
Contains an N-terminal 6-His tag, Ser47 - Ser368
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Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-604
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 5 mM DTT and 1 mM EDTA |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: USP18
Ubiquitin-like carboxyl-terminal hydrolase 18 (USP18) is a specialized cysteine protease with a predicted molecular weight of 42 kDa. USP18 from Mus musculus is a member of the peptidase C19 family and shares 70% amino acid identity with its human ortholog. USP18 plays and important role in antiviral responses and interferon-mediated signaling pathways. Mice lacking the gene (USP18-/-) are hypersensitive to interferon, have shortened lifespans and pathologic abnormalaties of the brain. While it was initially speculated that the cause of these phenotypes stems from the accumulaiton of ISG15-conjugated intracellular proteins (a target for USP18 catalytic function), it was later demonstrated that USP18 has multiple roles, at least one of which is independent of its ISGylase activity.
- Goldmann, T. et al. (2015) EMBO J. 34: 1612
- Ketscher, L. & Knobeloch, K.P. (2015) Cytokine 76: 569
- Knobeloch, K.P. et al. (2005) Mol. Cell Biol. 25: 11030
- Speer, S.D. et al. (2016) Nat. Commun. doi: 10.1038/ncomms11496
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