Recombinant Mouse His6-USP18 Protein, CF

Catalog # Availability Size / Price Qty
E-604-050
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Recombinant Mouse His6-USP18 Protein, CF Summary

Product Specifications

Purity
>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Reaction conditions will need to be optimized for each specific application.  We recommend an initial recombinant murine USP18 concentration of 100-250 nM when using ISG15-AMC (UL-553) as a substrate.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse USP18 protein
Contains an N-terminal 6-His tag, Ser47 - Ser368
Accession #
Predicted Molecular Mass
38 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-604

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 5 mM DTT and 1 mM EDTA
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: USP18

Ubiquitin-like carboxyl-terminal hydrolase 18 (USP18) is a specialized cysteine protease with a predicted molecular weight of 42 kDa.  USP18 from Mus musculus is a member of the peptidase C19 family and shares 70% amino acid identity with its human ortholog.  USP18 plays and important role in antiviral responses and interferon-mediated signaling pathways.  Mice lacking the gene (USP18-/-) are hypersensitive to interferon, have shortened lifespans and pathologic abnormalaties of the brain.  While it was initially speculated that the cause of these phenotypes stems from the accumulaiton of ISG15-conjugated intracellular proteins (a target for USP18 catalytic function), it was later demonstrated that USP18 has multiple roles, at least one of which is independent of its ISGylase activity.

References
  1. Goldmann, T. et al. (2015) EMBO J.  34: 1612
  2. Ketscher, L. & Knobeloch, K.P. (2015) Cytokine  76: 569
  3. Knobeloch, K.P. et al. (2005) Mol. Cell Biol.  25: 11030
  4. Speer, S.D. et al. (2016) Nat. Commun.  doi: 10.1038/ncomms11496
Long Name
Ubiquitin Specific Peptidase 18
Entrez Gene IDs
11274 (Human); 24110 (Mouse); 312688 (Rat)
Alternate Names
EC 3.1.2.15; EC 3.4.19.-; hUBP43; ISG15-specific-processing protease; ISG43; ISG43UBP43; ubiquitin specific peptidase 18; ubiquitin specific protease 18; ubl carboxyl-terminal hydrolase 18; ubl thioesterase 18,43 kDa ISG15-specific protease; Ubl thiolesterase 18; UBP43; USP18

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