Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
796-IC-050
Product Details
Citations (53)
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Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to rmICAM/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), approximately 65-95% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse ICAM-1/CD54 protein
Mouse ICAM-1
(Gln28 - Asn485)
Accession # Q3U8M7
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gln28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
76.8 kDa (monomer)
SDS-PAGE
110-130 kDa, reducing conditions

Product Datasheets

796-IC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 0.4 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

References
  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Citations for Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

53 Citations: Showing 1 - 10
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  1. Constitutive activation of WASp in X-linked neutropenia renders neutrophils hyperactive
    Authors: M Keszei, J Record, JS Kritikou, H Wurzer, C Geyer, M Thiemann, P Drescher, H Brauner, L Köcher, J James, M He, MA Baptista, CI Dahlberg, A Biswas, S Lain, DP Lane, W Song, K Pütsep, P Vandenberg, SB Snapper, LS Westerberg
    J. Clin. Invest., 2018;128(9):4115-4131.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis
    Authors: A Farinazzo, S Angiari, E Turano, E Bistaffa, S Dusi, S Ruggieri, R Bonafede, R Mariotti, G Constantin, B Bonetti
    Sci Rep, 2018;8(1):7473.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  3. Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells
    Authors: JG Borger, VL Morrison, A Filby, C Garcia, LM Uotila, F Simbari, SC Fagerholm, R Zamoyska
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. PDE8 controls CD4(+) T cell motility through the PDE8A-Raf-1 kinase signaling complex
    Authors: CP Basole, RK Nguyen, K Lamothe, A Vang, R Clark, GS Baillie, PM Epstein, S Brocke
    Cell. Signal., 2017;40(0):62-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation
    Authors: BR Gittens, JV Bodkin, S Nourshargh, M Perretti, D Cooper
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Dual Biomembrane Force Probe enables single-cell mechanical analysis of signal crosstalk between multiple molecular species
    Authors: L Ju, Y Chen, K Li, Z Yuan, B Liu, SP Jackson, C Zhu
    Sci Rep, 2017;7(1):14185.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. MFG-E8-derived peptide attenuates adhesion and migration of immune cells to endothelial cells
    Authors: Y Hirano, WL Yang, M Aziz, F Zhang, B Sherry, P Wang
    J. Leukoc. Biol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. ADAP plays a pivotal role in CD4+ T cell activation but is only marginally involved in CD8+ T cell activation, differentiation, and immunity to pathogens
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Glycogen storage disease type Ib neutrophils exhibit impaired cell adhesion and migration
    Authors: Janice Chou
    Biochem. Biophys. Res. Commun., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Pyk2 Controls Integrin-Dependent CTL Migration through Regulation of De-Adhesion
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Opposing effects of actin signaling and LFA-1 on establishing the affinity threshold for inducing effector T-cell responses in mice
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Blood
    Applications: Bioassay
  13. Prolonged exposure to volatile anesthetic isoflurane worsens the outcome of polymicrobial abdominal sepsis
    Authors: Sophia Koutsogian
    Toxicol. Sci, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Mst1 Kinase Regulates the Actin-Bundling Protein L-Plastin To Promote T Cell Migration
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Post-arrest stalling rather than crawling favors CD8(+) over CD4(+) T-cell migration across the blood-brain barrier under flow in vitro
    Authors: Henriette Rudolph
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. WNK1 kinase balances T cell adhesion versus migration in vivo
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Platelets Guide Leukocytes to Their Sites of Extravasation
    PLoS Biol, 2016;14(5):e1002459.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Aged neutrophils contribute to the first line of defense in the acute inflammatory response
    Blood, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  19. Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the alpha4beta1-integrin.
    Authors: Martin-Blondel G, Pignolet B, Tietz S, Yshii L, Gebauer C, Perinat T, Van Weddingen I, Blatti C, Engelhardt B, Liblau R
    Eur J Immunol, 2015;45(12):3302-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. CRK proteins selectively regulate T cell migration into inflamed tissues.
    Authors: Huang, Yanping, Clarke, Fiona, Karimi, Mobin, Roy, Nathan H, Williamson, Edward K, Okumura, Mariko, Mochizuki, Kazuhiro, Chen, Emily J, Park, Tae-Ju, Debes, Gudrun F, Zhang, Yi, Curran, Tom, Kambayashi, Taku, Burkhardt, Janis K
    J Clin Invest, 2015;125(3):1019-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Mutation in the CD45 inhibitory wedge modulates integrin activation and leukocyte recruitment during inflammation.
    Authors: Germena G, Volmering S, Sohlbach C, Zarbock A
    J Immunol, 2015;194(2):728-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Multi-Inhibitory Effects of A2A Adenosine Receptor Signaling on Neutrophil Adhesion Under Flow.
    Authors: Yago T, Tsukamoto H, Liu Z, Wang Y, Thompson L, McEver R
    J Immunol, 2015;195(8):3880-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Blocking neutrophil integrin activation prevents ischemia-reperfusion injury.
    Authors: Yago T, Petrich B, Zhang N, Liu Z, Shao B, Ginsberg M, McEver R
    J Exp Med, 2015;212(8):1267-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse.
    Authors: Na B, Kim H, Piragyte I, Oh H, Kwon M, Akber U, Lee H, Park D, Song W, Park Z, Im S, Rho M, Hyun Y, Kim M, Jun C
    J Cell Biol, 2015;209(1):143-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Adhesion
  25. alphaPIX RhoGEF supports positive selection by restraining migration and promoting arrest of thymocytes.
    Authors: Korthals M, Schilling K, Reichardt P, Mamula D, Schluter T, Steiner M, Langnase K, Thomas U, Gundelfinger E, Premont R, Tedford K, Fischer K
    J Immunol, 2014;192(7):3228-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis.
    Authors: Dalli, Jesmond, Norling, Lucy V, Montero-Melendez, Trinidad, Federici Canova, Donata, Lashin, Hazem, Pavlov, Anton M, Sukhorukov, Gleb B, Hinds, Charles, Perretti, Mauro
    EMBO Mol Med, 2014;6(1):27-42.
    Species: Mouse
    Sample Types: Plasma
    Applications: Bioassay
  27. Endothelial junctional adhesion molecule-a guides monocytes into flow-dependent predilection sites of atherosclerosis.
    Authors: Schmitt, Martin M, Megens, Remco T, Zernecke, Alma, Bidzhekov, Kiril, van den Akker, Nynke M, Rademakers, Timo, van Zandvoort, Marc A, Hackeng, Tilman M, Koenen, Rory R, Weber, Christia
    Circulation, 2014;129(1):66-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via beta2 integrins (CD11/CD18).
    Authors: Weckbach L, Gola A, Winkelmann M, Jakob S, Groesser L, Borgolte J, Pogoda F, Pick R, Pruenster M, Muller-Hocker J, Deindl E, Sperandio M, Walzog B
    Blood, 2014;123(12):1887-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Mst1 directs Myosin IIa partitioning of low and higher affinity integrins during T cell migration.
    Authors: Xu, Xiaolu, Jaeger, Emily R, Wang, Xinxin, Lagler-Ferrez, Erica, Batalov, Serge, Mathis, Nancy L, Wiltshire, Tim, Walker, John R, Cooke, Michael, Sauer, Karsten, Huang, Yina H
    PLoS ONE, 2014;9(8):e105561.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Geometrically controlled asymmetric division of CD4+ T cells studied by immunological synapse arrays.
    Authors: Jung H, Song K, Chang J, Doh J
    PLoS ONE, 2014;9(3):e91926.
    Species: N/A
    Sample Types: Complex Sample Type
    Applications: Bioassay
  31. Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness.
    Authors: Wang X, Rodda L, Bannard O, Cyster J
    J Immunol, 2014;192(10):4601-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. CD45 ligation expands Tregs by promoting interactions with DCs.
    Authors: Camirand, Geoffrey, Wang, Ying, Lu, Yuning, Wan, Yisong Y, Lin, Yan, Deng, Songyan, Guz, Galip, Perkins, David L, Finn, Patricia, Farber, Donna L, Flavell, Richard, Shlomchik, Warren D, Lakkis, Fadi G, Rudd, Christop, Rothstein, David M
    J Clin Invest, 2014;124(10):4603-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. The Src family kinases Hck, Fgr, and Lyn are critical for the generation of the in vivo inflammatory environment without a direct role in leukocyte recruitment.
    Authors: Kovacs M, Nemeth T, Jakus Z, Sitaru C, Simon E, Futosi K, Botz B, Helyes Z, Lowell C, Mocsai A
    J Exp Med, 2014;211(10):1993-2011.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Development of central nervous system autoimmunity is impaired in the absence of Wiskott-Aldrich syndrome protein.
    Authors: Bosticardo, Marita, Musio, Silvia, Fontana, Elena, Angiari, Stefano, Draghici, Elena, Constantin, Gabriela, Poliani, Pietro L, Pedotti, Rosetta, Villa, Anna
    PLoS ONE, 2014;9(1):e86942.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Regulation of T cell motility in vitro and in vivo by LPA and LPA2.
    Authors: Knowlden S, Capece T, Popovic M, Chapman T, Rezaee F, Kim M, Georas S
    PLoS ONE, 2014;9(7):e101655.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Cytoskeletal regulatory gene expression and migratory properties of B-cell progenitors are affected by the ETV6-RUNX1 rearrangement.
    Authors: Palmi C, Fazio G, Savino A, Procter J, Howell L, Cazzaniga V, Vieri M, Longinotti G, Brunati I, Andre V, Della Mina P, Villa A, Greaves M, Biondi A, D'Amico G, Ford A, Cazzaniga G
    Mol Cancer Res, 2014;12(12):1796-806.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Chemokine-coupled beta2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis.
    Authors: Morrison A, Yarovinsky T, Young B, Moraes F, Ross T, Ceneri N, Zhang J, Zhuang Z, Sinusas A, Pardi R, Schwartz M, Simons M, Bender J
    J Exp Med, 2014;211(10):1957-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  38. A circadian clock gene, Rev-erbalpha, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression.
    Authors: Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, Taniguchi N, Ohno H, Kizaki T
    J Immunol, 2014;192(1):407-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Distinct structural and catalytic roles for Zap70 in formation of the immunological synapse in CTL.
    Authors: Jenkins, Misty R, Stinchcombe, Jane C, Au-Yeung, Byron B, Asano, Yukako, Ritter, Alex T, Weiss, Arthur, Griffiths, Gillian
    Elife, 2014;3(0):e01310.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Phospholipase D1 mediates lymphocyte adhesion and migration in experimental autoimmune encephalomyelitis.
    Authors: Gobel K, Schuhmann M, Pankratz S, Stegner D, Herrmann A, Braun A, Breuer J, Bittner S, Ruck T, Wiendl H, Kleinschnitz C, Nieswandt B, Meuth S
    Eur J Immunol, 2014;44(8):2295-305.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Mammalian diaphanous-related formin 1 regulates GSK3beta-dependent microtubule dynamics required for T cell migratory polarization.
    Authors: Dong B, Zhang S, Gao W, Su H, Chen J, Jin F, Bhargava A, Chen X, Jorgensen L, Alberts A, Zhang J, Siminovitch K
    PLoS ONE, 2013;8(11):e80500.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Janus kinases 1 and 2 regulate chemokine-mediated integrin activation and naive T-cell homing.
    Authors: Perez-Rivero G, Cascio G, Soriano S, Sanz A, de Guinoa J, Rodriguez-Frade J, Gomariz R, Holgado B, Cabanas C, Carrasco Y, Stein J, Mellado M
    Eur J Immunol, 2013;43(7):1745-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  43. The transcription factor NFAT exhibits signal memory during serial T cell interactions with antigen-presenting cells.
    Authors: Marangoni F, Murooka T, Manzo T, Kim E, Carrizosa E, Elpek N, Mempel T
    Immunity, 2013;38(2):237-49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  44. CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation.
    Authors: Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H
    J Immunol, 2013;190(7):3696-705.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  45. PKCtheta regulates T cell motility via ezrin-radixin-moesin localization to the uropod.
    Authors: Cannon J, Asperti-Boursin F, Letendre K, Brown I, Korzekwa K, Blaine K, Oruganti S, Sperling A, Moses M
    PLoS ONE, 2013;8(11):e78940.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  46. Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels.
    Authors: Hyun YM, Sumagin R, Sarangi PP
    J. Exp. Med., 2012;209(7):1349-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Vitronectin induces phosphorylation of ezrin/radixin/moesin actin-binding proteins through binding to its novel neuronal receptor telencephalin.
    Authors: Furutani Y, Kawasaki M, Matsuno H, Mitsui S, Mori K, Yoshihara Y
    J Biol Chem, 2012;287(46):39041-9.
    Species: N/A
    Sample Types: N/A
    Applications: Surface Plasmon Resonance
  48. Leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction induces a novel genetic signature resulting in T-cells refractory to transforming growth factor-beta signaling.
    Authors: Verma N, Dempsey E, Long A, Davies A, Barry S, Fallon P, Volkov Y, Kelleher D
    J Biol Chem, 2012;287(32):27204-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Difference in Th1 and Th17 lymphocyte adhesion to endothelium.
    Authors: Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH
    J. Immunol., 2012;188(3):1421-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  50. IL-12 enhances CTL synapse formation and induces self-reactivity.
    Authors: Markiewicz MA, Wise EL, Buchwald ZS, Cheney EE, Hansen TH, Suri A, Cemerski S, Allen PM, Shaw AS
    J. Immunol., 2009;182(3):1351-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Aberrant activation of integrin alpha4beta7 suppresses lymphocyte migration to the gut.
    Authors: Park EJ, Mora JR, Carman CV, Chen J, Sasaki Y, Cheng G, von Andrian UH, Shimaoka M
    J. Clin. Invest., 2007;117(9):2526-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  52. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
    Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
    J. Leukoc. Biol., 2007;81(3):748-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Prostaglandin D2 mediates neuronal damage by amyloid-beta or prions which activates microglial cells.
    Authors: Bate C, Kempster S, Williams A
    Neuropharmacology, 2006;50(2):229-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Ctrl

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