IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta.
Recombinant Mouse IL-1 beta/IL-1F2 Protein
R&D Systems | Catalog # 401-ML
Key Product Details
- R&D Systems E. coli-derived Recombinant Mouse IL-1 beta/IL-1F2 Protein (401-ML)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Val118-Ser269, with an N-terminal Met
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 2-10 pg/mL.
Reviewed Applications
Read 8 reviews rated 4.9 using 401-ML in the following applications:
Scientific Data Images for Recombinant Mouse IL-1 beta/IL-1F2 Protein
Recombinant Mouse IL-1 beta/IL-1F2 Protein Bioactivity
Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 2‑10 pg/mL.
Recombinant Mouse IL-1 beta/IL-1F2 Protein SDS-PAGE
1 μg/lane of Recombinant Mouse IL1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 401-ML
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 401-ML/CF
| Formulation | Supplied as a 0.2 μm filtered solution in PBS. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: IL-1 beta/IL-1F2
References
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Gray, P.W. et al. (1986) J. Immunol. 137:3644.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional IL-1 beta/IL-1F2 Products
Product Documents for Recombinant Mouse IL-1 beta/IL-1F2 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse IL-1 beta/IL-1F2 Protein
For research use only
Related Research Areas
Citations for Recombinant Mouse IL-1 beta/IL-1F2 Protein
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Customer Reviews for Recombinant Mouse IL-1 beta/IL-1F2 Protein (8)
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Customer Images
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Application: TublogenesisVerified Customer | Posted 07/25/2023
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 10/05/2020
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 03/17/2020
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Application: In vivo studyVerified Customer | Posted 03/06/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/15/2020
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Application: Inflammatory stimulantVerified Customer | Posted 12/15/2017
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 08/02/2017
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Application: Apoptosis assayVerified Customer | Posted 01/13/2016I treat the IL-1 beta with IFN-gamma in beta-Tc3 cells. Cell death rate is almost 30% for 48 hrs.
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FAQs for Recombinant Mouse IL-1 beta/IL-1F2 Protein
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Q: Can Recombinant Mouse IL-1 beta/IL-1F2 Protein (Catalog # 401-ML) be used in rat or human cells?
A: Bioactivity of Recombinant Mouse IL-1 beta Protein (Catalog # 401-ML) has only been evaluated using mouse cells. We offer both Recombinant Human IL-1 beta Protein (Catalog # 201-IL) and Recombinant Rat IL-1 beta Protein (Catalog # 501-RL) that we recommend for use in human and rat assay systems.
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Q: Does IL-1 beta induce NO in smooth muscle?
A: Yes it does, based on the literature.
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Q: Can Recombinant Mouse IL-1 beta/IL-1F2 Protein (Catalog # 401-ML) be used in rat or human cells?
A: Bioactivity of Recombinant Mouse IL-1 beta Protein (Catalog # 401-ML) has only been evaluated using mouse cells. We offer both Recombinant Human IL-1 beta Protein (Catalog # 201-IL) and Recombinant Rat IL-1 beta Protein (Catalog # 501-RL) that we recommend for use in human and rat assay systems.
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Q: Does IL-1 beta induce NO in smooth muscle?
A: Yes it does, based on the literature.
Associated Pathways
IL-1 Family Signaling Pathways
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Inflammasome Activation Pathways
NOD-like Receptor Signaling Pathways
Th17 Differentiation Pathway
Toll-Like Receptor Signaling Pathways
