Recombinant Mouse Integrin alpha M beta 2 Protein, CF

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7959-AM-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Integrin alpha M beta 2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with ICAM-1. The ED50 for this effect is 0.4-1.6 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha M beta 2 protein
Mouse Integrin alpha M
(Phe17-Asn1105)
Accession # NP_032427
His-Pro GGGSGGGS Acidic Tail 6-His tag
Mouse Integrin beta 2
(Gln24-Asn702)
Accession # P11835
His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Phe17 (Integrin alpha M) & Gln24 predicted, No results obtained: sequencing might be blocked (Integrin beta 2)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
130 kDa (Integrin alpha M) & 83 kDa (Integrin beta 2)
SDS-PAGE
155-180 kDa & 95-105 kDa, reducing conditions

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7959-AM

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7959-AM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Integrin alpha M beta 2

Integrin  alpha M beta 2, also called MAC‑1 or complement receptor type 3 (CR3), is one of three leukocyte  beta 2 integrins. The non‑covalent heterodimer of 170 kDa alpha M/CD11b and 95 kDa beta 2/CD18 integrin subunits is expressed mainly on myeloid and natural killer cells (1‑6). The alpha M vWFA or I‑domain, which contains adhesion sites, forms the N‑terminal head region with the alpha M beta ‑propeller and the beta 2 vWFA domain. Unlike most integrins, the calf domain of alpha M is lectin‑like and binds carbohydrates (7). Each subunit has a transmembrane sequence and a short cytoplasmic tail. The 1089 amino acid (aa) mouse alpha M/CD11b ECD shares 87% aa sequence identity with rat, and 70‑74% with human, equine, bovine, feline, and canine  alpha M ECD. A potential mouse alpha M isoform lacks 117 aa within the beta ‑propeller. The 679 aa mouse  beta 2/CD18 ECD shares 91% aa sequence identity with rat, and 80‑82% with human, bovine, canine, and porcine beta 2 ECD. Like other integrins, alpha M beta 2 has multiple activation states (1‑3). In the presence of divalent cations and "inside‑out" signaling, alpha M beta 2 is fully active and extended. In the inactive state, the heterodimer flexes in the center at the alpha M thigh and calf domains and beta 2 I‑EGF domains, impeding access to adhesion sites. Active alpha M beta 2 binds an unusually large number of adhesion partners, including the complement opsonin fragment iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DC-SIGN (3, 4, 7). alpha M beta 2 lectin-like adhesion partners include heparin, bacterial lipopolysaccharides, and GPI‑linked glycoproteins such as uPAR and Fc gamma RIIIB (3, 7). Binding of platelet JAM‑C links platelets with myeloid and dendritic cell (DC) alpha M beta 2 and recruits these cells to inflamed or injured endothelium, while neutrophil alpha M beta 2 adheres to RAGE on inflamed endothelium; both are atherogenic events (3, 8, 9). However, activation of alpha M beta 2 inhibits alternative activation of macrophages and atherosclerotic foam cell formation (3, 10). alpha M beta 2 can either suppress or allow constitutive neutrophil apoptosis, depending on its ligand and activation state (3, 11, 12). Deletion of mouse  alpha M causes defects in neutrophil adhesion and degranulation, while mutations of human or mouse  beta 2 cause leukocyte adhesion deficiency (LAD‑1) and susceptibility to bacterial infections (3, 12, 13).

References
  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Tan, S.M. (2012) Biosci. Rep. 32:241.
  4. Pytela, R. (1988) EMBO J. 7:1371.
  5. Kishimoto, T. K. et al. (1987) Cell 48:681.
  6. Muto, S. et al. (1993) J. Clin. Immunol. 13:175.
  7. Xia, Y. et al. (2002) J. Immunol. 169:6417.
  8. Santoso, S. et al. (2002) J. Exp. Med. 196:679.
  9. Langer, H.F. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:1463.
  10. Yakubenko, V.P. et al. (2011) Circ. Res. 108:544.
  11. Pluskota, E. et al. (2008) J. Immunol. 181:3609.
  12. Coxon, A. et al. (1996) Immunity 5:653.
  13. Lu, H. et al. (1997) J. Clin. Invest. 99:1340.
Entrez Gene IDs
3684 (Human)
Alternate Names
Integrin alpha M beta 2

Citations for Recombinant Mouse Integrin alpha M beta 2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cryptococcus gattii evades CD11b-mediated fungal recognition by coating itself with capsular polysaccharides
    Authors: K Ueno, Y Otani, N Yanagihara, M Urai, A Nagamori, M Sato-Fukus, K Shimizu, N Saito, Y Miyazaki
    European Journal of Immunology, 2021;0(0):.
    Species: Fungi - C. gattii
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The Bordetella adenylate cyclase repeat-in-toxin (RTX) domain is immunodominant and elicits neutralizing antibodies.
    Authors: Wang X, Maynard J
    J Biol Chem, 2015;290(6):3576-91.

FAQs

  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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