Recombinant Mouse NCAM-1/CD56 Protein, CF

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6070-NC-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse NCAM-1/CD56 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. When 5 x 104 cells/well are added to mouse NCAM-1 coated plates (25 µg/mL with 100 µL/well), >20% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse NCAM-1/CD56 protein
Met1-Thr711, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu20
Predicted Molecular Mass
77.4 kDa
SDS-PAGE
105-140 kDa, reducing conditions

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6070-NC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6070-NC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: NCAM-1/CD56

Neural cell adhesion molecule 1 (NCAM-1; also CD56) is a membrane-bound glycoprotein that plays an important role in nervous system development and function (1, 2). Mature mouse NCAM-1 consists of a 692 amino acid (aa) extracellular domain (ECD) with five tandem Ig-like domains and two fibronectin type III domains, an 18 aa transmembrane segment, and a 386 aa cytoplasmic domain (3). Three major splice variants of NCAM-1 are expressed: the 180 kDa full length NCAM-180 isoform, the 140 kDa NCAM-140 isoform which lacks most of the cytoplasmic domain, and the 120 kDa GPI-anchored NCAM-120 isoform that includes the ECD only. Splicing is tissue specific and developmentally regulated (4 - 6). Within the ECD, mouse NCAM-1 shares 94% and 95% aa sequence identity with human and rat NCAM-1, respectively. It is expressed on neurons and glial cells, skeletal muscle, and immune NK cells (5, 7 - 9). NCAM-1 is extensively modified with polysialic acid (PSA) during development, but this addition is decreased in adult tissues (5, 6, 8). Polysialylation of NCAM-1 is retained in the adult hippocampus where it is important for synaptic plasticity and memory formation (10). The PSA moiety also participates in the binding of NCAM-1 to heparan sulfate proteoglycans and NCAM-1 mediated migration of olfactory neurons (11, 12). Proteolytic shedding of NCAM-1 liberates a soluble ECD fragment that can inhibit cortical neurite branching and growth (6). The NCAM-140 isoform is preferentially expressed on NK cells that robustly secrete cytokines upon activation (9, 13). Selective up‑regulation of the NCAM-140 isoform in a variety of tumors initiates epithelial-mesenchymal transition (EMT) and promotes tumor cell invasion (14, 15). Finally, NCAM-1 is known to interact with a number of transmembrane and extracellular molecules. NK cell NCAM-1 binds to T cell FGF R1, co-stimulating IL-2 production by T cells (16). NCAM-1 also forms a noncovalent membrane complex with GFR alpha 1, 2 and 4, generating a receptor for GDNF, NTN and PSP, respectively (17). And NCAM-1 is reported to form homophilic trans-interactions, and to interact with L1 CAM in cis, and with HSPGs (agrin and collagen XVIII) in trans. In general, these interactions are involved in cell adhesion, migration, and/or process extension (18).

References
  1. Schmid, R.S. and P.F. Maness (2008) Curr. Opin. Neurobiol. 18:245.
  2. Hansen, S.M. et al. (2008) Cell. Mol. Life Sci. 65:3809.
  3. Barthels, D. et al. (1987) EMBO J. 6:907.
  4. Gennarini, G. et al. (1986) J. Neurosci. 6:1983.
  5. Covault, J. et al. (1986) J. Cell Biol. 102:731.
  6. Brennaman, L.H. and P.F. Maness (2008) Mol. Cell. Neurosci. 37:781.
  7. Noble, M. et al. (1985) Nature 316:725.
  8. Chuong, C.M. and G.M. Edelman (1984) J. Neurosci. 4:2354.
  9. Lanier, L.L. et al. (1989) J. Exp. Med. 169:2233.
  10. Muller, D. et al. (1996) Neuron 17:413.
  11. Storms, S.D. and U. Rutishauser (1998) J. Biol. Chem. 273:27124.
  12. Tomasiewicz, H. et al. (1993) Neuron 11:1163.
  13. Cooper, M.A et al. (2001) Blood 97:3146.
  14. Gattenlohner, S. et al. (2009) Am. J. Pathol. 174:1160.
  15. Lehembre, F. et al. (2008) EMBO J. 27:2603.
  16. Kos, F.J. & Chin, C.S. (2002) Immunol. Cell Biol. 80:364.
  17. Paratcha, G. et al. (2003) Cell 113:867.
  18. Nielsen, J. et al. (2010) Adv. Exp. Med. Biol. 663:23.
Long Name
Neural Cell Adhesion Molecule
Entrez Gene IDs
4684 (Human); 17967 (Mouse); 24586 (Rat)
Alternate Names
CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM

Citation for Recombinant Mouse NCAM-1/CD56 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Neural cell-cell and cell-substrate adhesion through N-cadherin, N-CAM and L1.
    Authors: Wiertz RW, Marani E, Rutten WL
    J Neural Eng, 2011;8(4):046004.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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