Recombinant Mouse Reg4 Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT‑116 human colorectal carcinoma cells under low serum conditions. The ED50 for this effect is 2-10 µg/mL.
  • Source
    E. coli-derived mouse Reg4 protein
    Asp23-Thr157, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    16.2 kDa
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4780-RG
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 300 - 500 μg/mL in sterile, deionized water.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Immobilized Recombinant Mouse Reg4 (Catalog # 4780-RG) stimulates proliferation of HCT‑116 human colorectal carcinoma cells under low serum conditions. The ED50 for this effect is 2-10 µg/mL.
Background: Reg4

Reg4 (regenerating islet‑derived protein 4), also called Reg IV or RELP (Reg‑like protein), is a 17 kDa secreted glycoprotein belonging to the regenerating gene (Reg) family within the calcium (C‑type) dependent lectin superfamily, although carbohydrate binding of Reg4 is calcium‑independent (1 ‑ 4). Mouse Reg4 is synthesized as a 157 amino acid (aa) precursor with a 22 aa signal sequence and a 135 aa mature chain that contains a C‑type lectin‑like domain (CTLD) and two potential N‑linked glycosylation sites. Mouse Reg4 shares 68%, 88%, 64% and 64% aa sequence identity with human, rat, canine and porcine Reg4, respectively. Like other members of the regenerating gene family, Reg4 is preferentially expressed in the gastrointestinal (GI) tract (1, 2, 5). Reg4 expression is increased within or near inflammation, dysplasia and metaplasia of the GI epithelium, such as inflammatory bowel disease (Crohn’s disease and ulcerative colitis), colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and is often increased in the plasma in these conditions (1 ‑ 3, 5, 6). It is especially associated with neuroendocrine tumors in the GI, as well as some prostate, parathyroid, skin Merkel cell and lung small‑cell carcinomas (6 ‑ 8). Reg4 expression is induced by growth factors and promotes phosphorylation and activation of the EGF R (5, 7, 9). Tumor cells expressing Reg4 are generally more mitogenic, metastatic and resistant to apoptosis (10 ‑ 12).

  • References:
    1. Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
    2. Zhang, Y-W. et al. (2003) World J. Gastroenterol. 9:2635.
    3. Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
    4. Ho, M.R. et al. (2010) J. Mol. Biol. 402:682.
    5. Nanakin, A. et al. (2007) Lab. Invest. 87:304.
    6. Oue, N. et al. (2005) J. Pathol. 207:185.
    7. Ohara, S. et al. (2008) Cancer Sci. 99:1570.
    8. Heiskala, K. et al. (2010) Histol. Histopathol. 25:63.
    9. Bishnupuri K.S. et al. (2006) Gastroenterology 130:137.
    10. Rafa, L. et al. (2010) Int. J. Oncol. 36:689.
    11. Bishnupuri K.S. et al. (2010) Gastroenterology 138:616.
    12. Guo, Y. et al. (2010) Cancer Genet. Cytogenet. 199:38.
  • Long Name:
    Regenerating Islet-derived 4
  • Entrez Gene IDs:
    83998 (Human); 67709 (Mouse); 445583 (Rat)
  • Alternate Names:
    Gastrointestinal secretory protein; GISP; GISPREG-4; Reg IV; Reg4; regenerating gene type IV; regenerating islet-derived family, member 4; regenerating islet-derived protein 4; Regenerating islet-derived protein IV; REG-IV; RELP; RELPREG-like protein
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Reconstitution Buffers
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Reconstitution Buffer 7 (Deionized Water)

RB07
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