Recombinant Mouse Reg4 Protein, CF

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Recombinant Mouse Reg4 Protein Binding Activity
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Product Details
Citations (1)
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Recombinant Mouse Reg4 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT‑116 human colorectal carcinoma cells under low serum conditions. The ED50 for this effect is 2-10 µg/mL.
E. coli-derived mouse Reg4 protein
Asp23-Thr157, with an N-terminal Met
Accession #
N-terminal Sequence
Predicted Molecular Mass
16.2 kDa

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 - 500 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse Reg4 Protein Binding Activity View Larger

Immobilized Recombinant Mouse Reg4 (Catalog # 4780-RG) stimulates proliferation of HCT‑116 human colorectal carcinoma cells under low serum conditions. The ED50 for this effect is 2-10 µg/mL.

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Background: Reg4

Reg4 (regenerating islet‑derived protein 4), also called Reg IV or RELP (Reg‑like protein), is a 17 kDa secreted glycoprotein belonging to the regenerating gene (Reg) family within the calcium (C‑type) dependent lectin superfamily, although carbohydrate binding of Reg4 is calcium‑independent (1 ‑ 4). Mouse Reg4 is synthesized as a 157 amino acid (aa) precursor with a 22 aa signal sequence and a 135 aa mature chain that contains a C‑type lectin‑like domain (CTLD) and two potential N‑linked glycosylation sites. Mouse Reg4 shares 68%, 88%, 64% and 64% aa sequence identity with human, rat, canine and porcine Reg4, respectively. Like other members of the regenerating gene family, Reg4 is preferentially expressed in the gastrointestinal (GI) tract (1, 2, 5). Reg4 expression is increased within or near inflammation, dysplasia and metaplasia of the GI epithelium, such as inflammatory bowel disease (Crohn’s disease and ulcerative colitis), colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and is often increased in the plasma in these conditions (1 ‑ 3, 5, 6). It is especially associated with neuroendocrine tumors in the GI, as well as some prostate, parathyroid, skin Merkel cell and lung small‑cell carcinomas (6 ‑ 8). Reg4 expression is induced by growth factors and promotes phosphorylation and activation of the EGF R (5, 7, 9). Tumor cells expressing Reg4 are generally more mitogenic, metastatic and resistant to apoptosis (10 ‑ 12).

  1. Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
  2. Zhang, Y-W. et al. (2003) World J. Gastroenterol. 9:2635.
  3. Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
  4. Ho, M.R. et al. (2010) J. Mol. Biol. 402:682.
  5. Nanakin, A. et al. (2007) Lab. Invest. 87:304.
  6. Oue, N. et al. (2005) J. Pathol. 207:185.
  7. Ohara, S. et al. (2008) Cancer Sci. 99:1570.
  8. Heiskala, K. et al. (2010) Histol. Histopathol. 25:63.
  9. Bishnupuri K.S. et al. (2006) Gastroenterology 130:137.
  10. Rafa, L. et al. (2010) Int. J. Oncol. 36:689.
  11. Bishnupuri K.S. et al. (2010) Gastroenterology 138:616.
  12. Guo, Y. et al. (2010) Cancer Genet. Cytogenet. 199:38.
Long Name
Regenerating Islet-derived 4
Entrez Gene IDs
83998 (Human); 67709 (Mouse); 445583 (Rat)
Alternate Names
Gastrointestinal secretory protein; GISP; GISPREG-4; Reg IV; Reg4; regenerating gene type IV; regenerating islet-derived family, member 4; regenerating islet-derived protein 4; Regenerating islet-derived protein IV; REG-IV; RELP; RELPREG-like protein

Citation for Recombinant Mouse Reg4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Upregulation of Reg IV and Hgf mRNAs by Intermittent Hypoxia via Downregulation of microRNA-499 in Cardiomyocytes
    Authors: S Takasawa, A Itaya-Hiro, M Makino, A Yamauchi, S Sakuramoto, T Uchiyama, R Shobatake, Y Takeda, H Ota
    International Journal of Molecular Sciences, 2022;23(20):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture


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