Recombinant Mouse Siglec-E Fc Chimera Protein, CF

R&D Systems | Catalog # 5806-SL

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Siglec-E Fc Chimera Protein (5806-SL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

AAH23280

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Siglec-E protein
Mouse Siglec-E
(Met1-Phe355)
Accession # AAH23280		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained, N-sequencing might be blocked: Gln20 predicted

Predicted Molecular Mass

64.6 kDa (monomer)

SDS-PAGE

75-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Siglec‑E Fc Chimera is immobilized at 2 μg/mL (100 μL/well), it binds to Recombinant Mouse Galectin-3 (HEK293-expressed) Protein (Catalog # 9039-GAB) with an ED50 of 0.08-0.8 μg/mL.

Scientific Data Images for Recombinant Mouse Siglec-E Fc Chimera Protein, CF

Recombinant Mouse Siglec-E Fc Chimera Protein Binding Activity

Recombinant Mouse Siglec-E Fc Chimera Protein Binding Activity

When Recombinant Mouse Siglec-E Fc Chimera (Catalog # 5806-SL) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Galectin-3 (HEK293-expressed) Protein (Catalog # 9039-GAB) binds with an ED50 of 80-800 ng/mL.

Formulation, Preparation, and Storage

5806-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-E

Siglecs are sialic acid specific I‑type lectins that are characterized by an extracellular domain (ECD) with an N‑terminal Ig‑like V‑set domain followed by varying numbers of Ig‑like C2‑set domains (1, 2). Mouse Siglec‑E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 ‑ 85 kDa member of the CD33‑related subfamily of Siglecs. It consists of a 335 amino acid (aa) ECD with one Ig‑like V‑set domain and two Ig‑like C2‑set domains, a 21 aa transmembrane segment, and a 93 aa cytoplasmic domain that contains two immunoreceptor tyrosine‑based inhibitory motifs (ITIM) (3, 4). Rodent and primate Siglec gene families have significantly diverged, and Siglec‑9 is the most likely human ortholog of mouse Siglec‑E (1). Within the ECD, mouse Siglec‑E shares 56% and 80% aa sequence identity with human Siglec‑9 and rat Siglec‑E, respectively. Siglec‑E is expressed as a heavily N‑glycosylated disulfide‑linked homodimer and shows binding preference for disialic acids in the alpha 2‑8 linkage (3, 5). It is expressed on the surface of several hematopoietic cell types including neutrophils, NK cells, monocytes, peritoneal macrophages and B1 cells, and splenic myeloid dendritic cells and marginal zone B cells (5). Tyrosine phosphorylation of the cytoplasmic ITIMs mediates the association of Siglec‑E with the phosphatases SHP‑1 and SHP‑2 (3, 4). Siglec‑E is up‑regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists (6). Siglec‑E signaling negatively regulates the LPS‑induced production of TNF‑ alpha and IL‑6 by macrophages (4, 6). Its up‑regulation in macrophages parallels the development of endotoxin tolerance (6). Siglec‑E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell IL‑12 p40 production (7).

References

  1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
  2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
  3. Yu, Z. et al. (2001) Biochem. J. 353:483.
  4. Ulyanova, T. et al. (2001) J. Biol. Chem. 276:14451.
  5. Zhang, J.Q. et al. (2004) Eur. J. Immunol. 34:1175.
  6. Boyd, C.R. et al. (2009) J. Immunol. 183:7703.
  7. Erdmann, H. et al. (2009) Cell. Microbiol. 11:1600.

Long Name

Sialic Acid Binding Ig-like Lectin E

Alternate Names

SiglecE

Entrez Gene IDs

83382 (Mouse)

Gene Symbol

SIGLECE

UniProt

AAH23280

Additional Siglec-E Products

Product Documents for Recombinant Mouse Siglec-E Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse Siglec-E Fc Chimera Protein, CF

For research use only

Citations for Recombinant Mouse Siglec-E Fc Chimera Protein, CF

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