Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF
Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF Summary
|Mouse TGF-beta RI
Accession # BAA05023
|IEGRMD||Human IgG1 |
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: TGF-beta RI/ALK-5
TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase (1, 2). It contains a cysteine-rich extracellular domain (ECD), a transmembrane helix, and a C-terminal cytoplasmic kinase domain (3). Within the cytoplasmic domain there is also a short, conserved regulatory sequence known as the GS region that is N-terminal to the kinase domain (1). Within the ECD, mouse TGF-beta RI shares 98% and 90% amino acid sequence identity with rat and human TGF-beta RI, respectively. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII (1). Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3 (2, 4-6). These phosphorylated Smads form heteromeric complexes with Smad4, translocate to the nucleus, and regulate target gene transcription (2, 4-6). TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells (7). Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development (8). Mutations in TGF-beta RI have been identified in pancreatic, colorectal, ovarian, and head and neck cancers (9).
- Wrana, J.L. et al. (1994) Nature 370:341.
- Massagué, J. (2012) Nat. Rev. Mol. Cell Biol. 13:616.
- Huse, M. et al. (1999) Cell 96:425.
- Marcías-Silva, M. et al. (1996) Cell 87:1215.
- Zhang, Y. et al. (1996) Nature 383:168.
- Huse, M. et al. (2001) Mol. Cell 8:671.
- Larsson, J. et al. (2001) EMBO J. 20:1663.
- James, J.M. et al. (2013) Development 140:3903.
- Sheen, Y.Y. et al. (2013) Biomol. Ther. (Seoul) 21:323.
Citations for Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
J. Biol. Chem., 2011;286(34):30034-46.
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
ALK1 signaling regulates early postnatal lymphatic vessel development.
Authors: Niessen K, Zhang G, Ridgway JB, Chen H, Yan M
Sample Types: N/A
Applications: ELISA (Standard)
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