Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF Summary
|Mouse TNF RII/TNFRSF1B|
Accession # P25119
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse TNF RII/TNFRSF1B (Catalog # 9707-R2) inhibits Recombinant Mouse TNF-alpha aa 80-235 (Catalog # 410-MT) mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3-15 ng/mL.
Background: TNF RII/TNFRSF1B
Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta (1, 2). These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A (3). In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions (4). Mature mouse TNF RII contains an extracellular domain (ECD, aa 23 - 258) containing ten disulfide bonds, a transmembrane domain (aa 259-288), and a cytoplasmic domain (aa 289-474). Within the ECD, the mouse TNF RII shares 57% and 84% aa sequence identity to human and rat TNF RII. TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes, neurons, oligodendrocytes, cardiac myocytes, and thymocytes (5-7). TNF RII activation primarily initiates pro-inflammatory and pro-survival responses via NF kappa B-dependent signaling pathways (5, 8). However, under certain conditions, TNF RII signaling can induce apoptosis (5). Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors (9).
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- Faustman, D. and M. Davis (2010) Nat. Rev. Drug Discov. 9:482.
- Mason, A.T. et al. (1995) J. Leukoc. Biol. 58:249.
- Speeckaert, M.M. et al. (2012) Am. J. Nephrol. 36:261.
- Ihnatko, R. and M. Kubeš (2007) Gen. Physiol. Biophys. 26:159.
- Sennikov, S.V. et al. (2014) Mediators Inflamm. 2014:745909.
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