Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF

Name: Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 9707-R2
Price: 479 USD
Availability: InStock

R&D Systems | Catalog # 9707-R2

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems NS0-derived Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein (9707-R2)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P25119

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all TNF RII/TNFRSF1B Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TNF RII/TNFRSF1B protein

Mouse TNF RII/TNFRSF1B (Val23-Gly258) Accession # P25119	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val23

Predicted Molecular Mass

52 kDa

SDS-PAGE

66-76 kDa, reducing conditions

Activity

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.
The ED₅₀ for this effect is 3-15 ng/mL

Scientific Data Images for Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF

Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein Binding Activity

Recombinant Mouse TNF RII/TNFRSF1B (Catalog # 9707-R2) inhibits Recombinant Mouse TNF-alpha aa 80-235 (Catalog # 410-MT) mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 3-15 ng/mL.

Formulation, Preparation, and Storage

9707-R2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: TNF RII/TNFRSF1B

Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta (1, 2). These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A (3). In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions (4). Mature mouse TNF RII contains an extracellular domain (ECD, aa 23 - 258) containing ten disulfide bonds, a transmembrane domain (aa 259-288), and a cytoplasmic domain (aa 289-474). Within the ECD, the mouse TNF RII shares 57% and 84% aa sequence identity to human and rat TNF RII. TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes, neurons, oligodendrocytes, cardiac myocytes, and thymocytes (5-7). TNF RII activation primarily initiates pro-inflammatory and pro-survival responses via NF kappa B-dependent signaling pathways (5, 8). However, under certain conditions, TNF RII signaling can induce apoptosis (5). Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors (9).