Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9707-R2-050
Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein Binding Activity
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Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 3-15 ng/mL
Source
Mouse myeloma cell line, NS0-derived mouse TNF RII/TNFRSF1B protein
Mouse TNF RII/TNFRSF1B
(Val23-Gly258)
Accession # P25119
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52 kDa
SDS-PAGE
66-76 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9707-R2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Mouse TNF RII/TNFRSF1B Fc Chimera Protein Binding Activity View Larger

Recombinant Mouse TNF RII/TNFRSF1B (Catalog # 9707-R2) inhibits Recombinant Mouse TNF-alpha aa 80-235 (Catalog # 410-MT) mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3-15 ng/mL.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF RII/TNFRSF1B

Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta (1, 2). These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A (3). In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions (4). Mature mouse TNF RII contains an extracellular domain (ECD, aa 23 - 258) containing ten disulfide bonds, a transmembrane domain (aa 259-288), and a cytoplasmic domain (aa 289-474). Within the ECD, the mouse TNF RII shares 57% and 84% aa sequence identity to human and rat TNF RII. TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes, neurons, oligodendrocytes, cardiac myocytes, and thymocytes (5-7). TNF RII activation primarily initiates pro-inflammatory and pro-survival responses via NF kappa B-dependent signaling pathways (5, 8). However, under certain conditions, TNF RII signaling can induce apoptosis (5). Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors (9).

References
  1. Smith, C.A. et al. (1990) Science 248: 1019.
  2. Goodwin R.G. et al. (1991) Mol. Cell. Biol. 11:3020.
  3. Kohno, T. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8331.
  4. Yamada, Y. et al. (2000) Burns 26:239.
  5. Faustman, D. and M. Davis (2010) Nat. Rev. Drug Discov. 9:482.
  6. Mason, A.T. et al. (1995) J. Leukoc. Biol. 58:249.
  7. Speeckaert, M.M. et al. (2012) Am. J. Nephrol. 36:261.
  8. Ihnatko, R. and M. Kubeš (2007) Gen. Physiol. Biophys. 26:159.
  9. Sennikov, S.V. et al. (2014) Mediators Inflamm. 2014:745909.
Long Name
Tumor Necrosis Factor Receptor II
Entrez Gene IDs
7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)
Alternate Names
CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II

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