Recombinant Rat RAGE Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1616-RG-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Rat RAGE Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. rrRAGE/Fc Chimera immobilized at 5 µg/mL (100 µL/well) on a goat anti-human IgG Fc antibody-coated plate (0.5 µg/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA, Catalog # BT4127) with a linear range of 0.02-15 µg/mL.
Source
Mouse myeloma cell line, NS0-derived rat RAGE protein
Rat RAGE
(Gln24 - Ala342)
Accession # Q63495
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln24 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
60 kDa (monomer)
SDS-PAGE
80-90 kDa, reducing conditions

Product Datasheets

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1616-RG

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1616-RG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: RAGE/AGER

Advanced glycation endproducts (AGEs) are adducts formed by the non‑enzymatic glycation of macromolecules. AGE formation is accelerated in oxidative and hyperglycemic conditions, diabetes, renal failure, atherosclerosis, Alzheimer’s disease, arthritis, and in normal aging (1 - 5). Receptor for advanced glycation endproducts (RAGE) is a 35 kDa type I transmembrane protein belonging the immunoglobulin superfamily. Besides AGEs, RAGE binds beta -amyloid peptide, S100/calgranulin family proteins, HMGB1/amphoterin, and leukocyte integrins (6 ‑ 9). Mature rat RAGE consists of a 319 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain and two Ig-like C-type domains, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (10). Within the ECD, rat RAGE shares 79% and 91% aa sequence identity with human and mouse RAGE, respectively. In human, soluble forms of RAGE are generated by alternate splicing and are associated with multiple disease states (11, 12). RAGE is expressed in the embryonic central nervous system and on macrophages, monocytes, smooth muscle cells, and endothelial cells (13 ‑ 15). It is upregulated in response to AGE accumulation, and its activation induces a broad proinflammatory response (6, 15). The increased production of reactive oxygen species during inflammation promotes additional AGE formation and RAGE upregulation, a cycle that exacerbates diabetic complications and inflammation-induced tissue injury (2, 4).

References
  1. Schleicher, E. and U. Friess (2007) Kidney Int. Suppl. 106:S17.
  2. Herold, K. et al. (2007) J. Leukoc. Biol. 82:204.
  3. Thornalley, P.J. (2006) J. Ren. Nutr. 16:178.
  4. Goldin, A. et al. (2006) Circulation 114:597.
  5. Ramasamy, R. et al. (2005) Glycobiology 15:16R.
  6. Kislinger, T. et al. (1999) J. Biol. Chem. 274:31740.
  7. Yan, S.D. et al. (1996) Nature 382:685.
  8. Huttenen, H. et al. (2000) J. Biol. Chem. 275:40096.
  9. Chavakis, T. et al. (2003) J. Exp. Med. 198:1507.
  10. Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
  11. Yonekura H, et al. (2003) Biochem. J. 370:1097.
  12. Koyama, H. et al. (2007) Mol. Med. 13:625.
  13. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
  14. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
  15. Bierhaus, A. et al. (2006) Curr. Opin. Investig. Drugs 7:985.
Long Name
Receptor for Advanced Glycation End Products
Entrez Gene IDs
177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1

Citation for Recombinant Rat RAGE Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
    Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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