RelA/NFkB p65 NLS Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24541

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Bovine, Equine, Primate

Cited:

Human, Mouse

Predicted:

Canine (100%), Chicken (100%), Chimpanzee (100%), Drosophila (100%), Monkey (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RelA/NFkB p65 NLS Primary Antibodies »

Product Specifications

Immunogen

A portion of the NFkB p65 NLS nuclear localization signal (NLS) (between amino acids 250-330) was used as the immunogen for this antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RelA/NFkB p65 NLS Antibody - BSA Free

Western Blot: RelA/NFkB p65 NLS AntibodyBSA Free [NBP2-24541]

Western Blot: RelA/NFkB p65 NLS AntibodyBSA Free [NBP2-24541]

Western Blot: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in Jurkat lysate using p65 NLS antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 NLS Antibody [NBP2-24541] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-RelA/NFkB p65 NLS Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody Formalin-fixed, paraffin-embedded human liver stained with p65 NLS antibody at 10 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in formalin-fixed, paraffin-embedded human HeLa cells (top left) and LPS-stimulated HeLa cells (bottom left, right) using p65 NLS antibody at 5 ug/ml.

Applications for RelA/NFkB p65 NLS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-3 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RelA/NFkB p65 NLS

Alternate Names

MGC131774, NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)), Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65, p65 NLS, p65 Nuclear Localization Sequence, p65-NLS, v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Gene Symbol

RELA

Additional RelA/NFkB p65 NLS Products

Product Documents for RelA/NFkB p65 NLS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RelA/NFkB p65 NLS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RelA/NFkB p65 NLS Antibody - BSA Free

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Protocols

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