Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free

Novus Biologicals | Catalog # H00009572-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F6

Format

Azide and BSA Free
View all Rev-erb A alpha/NR1D1 Primary Antibodies »

Product Specifications

Immunogen

NR1D1 (AAH56148, 1 a.a. ~ 614 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQRHNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNGHRLCPTHVYAAPEGKAPANSPRQGNSKNVLLACPMNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVMFLSRTTYSLQELGAMGMGDLLSAMFDFSEKLNSLALTEEELGLFTAVVLVSADRSGMENSASVEQLQETLLRALRALVLKNRPLETSRFTKLLLKLPDLRTLNNMHSEKLLSFRVDAQ

Specificity

NR1D1 (4F6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6. Analysis of NR1D1 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Rev-erb-A-alpha-NR1D1-Antibody-4F6-Immunohistochemistry-Paraffin-H00009572-M02-img0024.jpg
Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 expression in transfected 293T cell line by NR1D1 monoclonal antibody (M02), clone 4F6. Lane 1: NR1D1 transfected lysate (66.8 KDa). Lane 2: Non-transfected lysate.
Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi ( Cat # H00009572-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D1 monoclonal antibody (M02), clone 4F6 (Cat # H00009572-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6 Analysis of NR1D1 expression in Hela S3 NE.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded mouse lung tissue using anti-NR1D1 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded human lung cancer tissue using anti-NR1D1 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of Rev-erb A alpha in paraffin embedded mouse lung sections using anti-Rev-erb A alpha antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Staining on human breast cancer tissue. Image from verified customer review.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
ELISA: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

ELISA: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

ELISA: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.
Rev-erb A alpha/NR1D1 Antibody (4F6)

Immunohistochemistry: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] -

Representative tissue microarray specimens stained for NR1D1. Immunoreactivity of representative tumor specimens is shown. Low and high NR1D1 expression groups are defined in methods. The low NR1D1 expression ranges from immunoreactive scores 2 to 6 (left (P2xI1), middle (P3xI1), and right (P3xI2)), and the high NR1D1 expression shows immunoreactive score 12 (all, P4xI3). P, percentage of stained tumor cells; I, intensity of staining. Scale bar, 50 μm
Rev-erb A alpha/NR1D1 Antibody (4F6)

Immunohistochemistry: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] -

Immunohistochemistry: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Representative tissue microarray specimens stained for NR1D1. Immunoreactivity of representative tumor specimens is shown. Low & high NR1D1 expression groups are defined in methods. The low NR1D1 expression ranges from immunoreactive scores 2 to 6 (left (P2xI1), middle (P3xI1), & right (P3xI2)), & the high NR1D1 expression shows immunoreactive score 12 (all, P4xI3). P, percentage of stained tumor cells; I, intensity of staining. Scale bar, 50 μm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31779659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.

Reviewed Applications

Read 4 reviews rated 4.3 using H00009572-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rev-erb A alpha/NR1D1

NR1D1 functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine

Alternate Names

Ear-1, NR1D1, Reverb A alpha, THRA1, THRAL

Entrez Gene IDs

9572 (Human)

Gene Symbol

NR1D1

OMIM

602408 (Human)

UniProt

AAH56148 (Human)

Additional Rev-erb A alpha/NR1D1 Products

Product Documents for Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free

Customer Reviews for Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
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3 Stars
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1 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Mouse lung paraffin embedded sections
    Species: Mouse
    Verified Customer | Posted 02/26/2015
    Nr1d1 expression in mouse lungs
    Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free H00009572-M02
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human lung cancer tissue
    Species: Human
    Verified Customer | Posted 02/22/2015
    Human lung cancer slides
    Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free H00009572-M02
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse lung paraffin embedded sections
    Species: Mouse
    Verified Customer | Posted 02/22/2015
    Mouse lung stained for Rev-erb alpha (Nr1d1)
    Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free H00009572-M02
  • Name: Hyelin Na
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human Breast Cancer Tissue
    Species: Human
    Verified Customer | Posted 09/26/2014
    breast cancer tissue
    Rev-erb A alpha/NR1D1 Antibody (4F6) - Azide and BSA Free H00009572-M02

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