RHAMM/CD168 Antibody [Alexa Fluor® 700]

Novus Biologicals | Catalog # NBP1-76538AF700

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM. The immunogen is located within amino acids 80 - 130 of RHAMM. Amino Acid Squence: ERGAQDRRIQDLETELEK

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27856308).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RHAMM/CD168 Antibody [Alexa Fluor® 700]

Flow Cytometry: RHAMM/CD168 Antibody [Alexa Fluor® 700] [NBP1-76538AF700] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in 500 uL PBS/BSA/EDTA and viable DAPI cells were analysed using a Galios flow cytometer. Flow cytometry image submitted by a verified customer review.

Applications for RHAMM/CD168 Antibody [Alexa Fluor® 700]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. RHAMM/CD168 Antibody [Alexa Fluor(R) 700] validated for Flow cytometry from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-76538AF700 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

50 mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RHAMM/CD168

The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.

Long Name

Receptor For Hyaluronan-Mediated Motility

Alternate Names

CD168, HMMR, IHABP

Gene Symbol

HMMR

Additional RHAMM/CD168 Products

Product Documents for RHAMM/CD168 Antibody [Alexa Fluor® 700]

Certificate of Analysis

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Product Specific Notices for RHAMM/CD168 Antibody [Alexa Fluor® 700]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RHAMM/CD168 Antibody [Alexa Fluor® 700]

Customer Reviews for RHAMM/CD168 Antibody [Alexa Fluor® 700] (1)

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Customer Images

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  • RHAMM/CD168 Antibody [Alexa Fluor® 700]
    Name: Moyra Lawrence
    Application: Flow Cytometry
    Sample Tested: Megakaryocytes
    Species: Homo sapiens and Human
    Verified Customer | Posted 10/11/2019
    47,000 CD41+CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20min at room temperature. Cells were then washed in 500uL PBS/BSA/EDTA and viable DAPI- cells were analysed using a Galios flow cytometer.
    47,000 CD41+CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20min at room temperature with the indicated quantity of antibody. Cells were then washed in 500uL PBS/BSA/EDTA and viable DAPI- cells were analysed using a Galios flow cytometer.
    RHAMM/CD168 Antibody [Alexa Fluor® 700] NBP1-76538AF700

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