RHAMM/CD168 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76538

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human, Mouse, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RHAMM/CD168 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM. The immunogen is located within amino acids 80 - 130 of RHAMM. Amino Acid Squence: ERGAQDRRIQDLETELEK

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27856308).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RHAMM/CD168 Antibody - BSA Free

RHAMM/CD168 Antibody - BSA Free

Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] -

Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168, 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: RHAMM/CD168 AntibodyBSA Free [NBP1-76538]

Western Blot: RHAMM/CD168 AntibodyBSA Free [NBP1-76538]

Western Blot: RHAMM/CD168 Antibody [NBP1-76538] - Analysis in rat stomach tissue lysate with antibody at 0.5 ug/mL.
Immunohistochemistry: RHAMM/CD168 Antibody - BSA Free [NBP1-76538]

Immunohistochemistry: RHAMM/CD168 Antibody - BSA Free [NBP1-76538]

Immunohistochemistry: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue.
Flow Cytometry: RHAMM/CD168 Antibody - BSA Free [NBP1-76538]

Flow Cytometry: RHAMM/CD168 Antibody - BSA Free [NBP1-76538]

Flow Cytometry: RHAMM/CD168 Antibody [NBP1-76538] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in 500 uL PBS/BSA/EDTA and viable DAPI cells were analysed using a Galios flow cytometer. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 700 version of this antibody.
RHAMM/CD168 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] -

Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Immunofluorescence of RHAMM/CD168 in human stomach tissue with RHAM antibody at 20 ug/mL.

Applications for RHAMM/CD168 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP1-76538 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RHAMM/CD168

The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.

Long Name

Receptor For Hyaluronan-Mediated Motility

Alternate Names

CD168, HMMR, IHABP

Gene Symbol

HMMR

UniProt

NP_036617

Additional RHAMM/CD168 Products

Product Documents for RHAMM/CD168 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RHAMM/CD168 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RHAMM/CD168 Antibody - BSA Free

Customer Reviews for RHAMM/CD168 Antibody - BSA Free (1)

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  • RHAMM/CD168 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Hippocampal Cell Suspension
    Species: Mouse
    Verified Customer | Posted 01/25/2018
    See experimental details.
    Used antibody for FACS. 5 x 10^6 Mouse primary hippocampal cells suspended in complete neurosphere supplement media at 4' C. Cells are not permeabilized. Primary antibody used at 1:200 dilution for an hour on ice. Secondary goat antibody diluted to 1:2000 used. BD FACS Aria Fusion Sorter was used to analyze the sample. Excitation wavelength 488nm. Emission Filter 530/30. Events acquired ~70,000. Controls used were as follows: 1) No staining without Propidium Iodide. 2)No staining with Propidium Iodide. 3)Secondary only with Propidium Iodide. Rhamm only with Propidium Iodide.
    RHAMM/CD168 Antibody - BSA Free NBP1-76538

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