Rhodopsin Antibody (4D2) - BSA Free

Novus Biologicals | Catalog # NBP2-59690

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Key Product Details

Species Reactivity

Validated:

Amphibian, Avian, Fish, Mammal, Zebrafish

Cited:

Human, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, ELISA, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D2

Format

BSA Free
View all Rhodopsin Primary Antibodies »

Product Specifications

Immunogen

Bovine Rhodopsin

Reactivity Notes

Extinct Spiny Shark (Acanthodes bridgei). Zebrafish reactivity reported from a verified customer review.

Localization

Membrane

Specificity

Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rhodopsin Antibody (4D2) - BSA Free

Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690]

Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690]

Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.
Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690]

Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690]

Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GCL ganglion cell layer..
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690]

Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690]

Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug.. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000.
Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690]

Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690]

Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690] - Zebrafish retina (outer nuclear layer). PFA and methanol fixed 7 um sections. Rhodopsin antibody O/N incubation at 1:500. IHC-Fr image submitted by a verified customer review.

Applications for Rhodopsin Antibody (4D2) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000

Western Blot

1:1000
Application Notes
1 ug/mL of this antibody was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Rhodopsin antibody validated for IHC-Fr from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-59690 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rhodopsin

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Long Name

Rhodopsin

Alternate Names

OPN2, Opsin-2, RHO

Gene Symbol

RHO

Additional Rhodopsin Products

Product Documents for Rhodopsin Antibody (4D2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Rhodopsin Antibody (4D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rhodopsin Antibody (4D2) - BSA Free

Customer Reviews for Rhodopsin Antibody (4D2) - BSA Free (1)

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Customer Images

Showing  1 - 1 of 1 review Showing All
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  • Rhodopsin Antibody (4D2)
    Name: Sanne Broekman
    Application: Immunohistochemistry-Frozen
    Sample Tested: Retina (outer nuclear layer)
    Species: Zebrafish
    Verified Customer | Posted 08/29/2019
    Zebrafish eye 5dpf
    o/n PFA and Methanol fixed 7um sections Put slides in 0.1% PBS-Tween 20 1-2 minutes In 10mM sodium citrate pH 8.5 autoclave (1 min at 121°C, door open at 99⁰C ) Put back in 0.1% PBS-Tween20 Wash 3 x 5min 0.1% PBS-Tween20 1 hour block solution (10% NFDM in 0.1% PBS-Tween20) Overnight with Primary antibody 1:500- 1:5000 in block solution Wash 3 x 10 min 0.1% PBS-Tween20 2 hour incubation with Secondary antibody (1:500) + DAPI (1:10000) in block solution Wash 3 X 10 min 0.1% PBS-Tween20 Enclose with prolong gold antifade Store in dark at 4°C.
    Rhodopsin Antibody (4D2) - BSA Free NBP2-59690
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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