Rhot1 Antibody (4H4) - Azide and BSA Free

Novus Biologicals | Catalog # H00055288-M01

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4H4

Format

Azide and BSA Free
View all Miro1/Rhot1 Primary Antibodies »

Product Specifications

Immunogen

RHOT1 (NP_060777, 483 a.a. ~ 580 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYP

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30126943). Other species not tested.

Specificity

RHOT1 - ras homolog gene family, member T1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Rhot1 Antibody (4H4) - Azide and BSA Free

Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]

Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]

Rhot1-Antibody-4H4-Western-Blot-H00055288-M01-img0005.jpg
Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]

Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]

Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - RHOT1 monoclonal antibody (M01), clone 4H4 Analysis of RHOT1 expression in Hela S3 NE.
ELISA: Rhot1 Antibody (4H4) [H00055288-M01]

ELISA: Rhot1 Antibody (4H4) [H00055288-M01]

ELISA: Rhot1 Antibody (4H4) [H00055288-M01] - Detection limit for recombinant GST tagged RHOT1 is approximately 0.3ng/ml as a capture antibody.

Applications for Rhot1 Antibody (4H4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Miro1/Rhot1

Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution

Long Name

Mitochondrial Rho (MIRO) GTPase 1

Alternate Names

ARHT1, Rhot1

Entrez Gene IDs

55288 (Human)

Gene Symbol

RHOT1

UniProt

NP_060777 (Human)

Additional Miro1/Rhot1 Products

Product Documents for Rhot1 Antibody (4H4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Rhot1 Antibody (4H4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rhot1 Antibody (4H4) - Azide and BSA Free

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Protocols

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FAQs

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