RNA polymerase I termination factor Antibody (2F4D8) - BSA Free

Novus Biologicals | Catalog # NBP3-27122

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F4D8

Format

BSA Free
View all RNA polymerase I termination factor Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RNA polymerase I termination factor (AA: 1-150) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNA polymerase I termination factor Antibody (2F4D8) - BSA Free

Immunohistochemistry: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

Immunohistochemistry: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Analysis of paraffin-embedded rectum cancer tissues using RNA polymerase I termination factor mouse mAb with DAB staining.

Western Blot: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

Western Blot: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Analysis using RNA polymerase I termination factor mAb against human RNA polymerase I termination factor (AA: 1-150) recombinant protein. (Expected MW is 43.5 kDa)

ELISA: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

ELISA: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

Western Blot: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Analysis using RNA polymerase I termination factor mAb against HEK293 (1) and RNA polymerase I termination factor (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

Immunocytochemistry/Immunofluorescence: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Analysis of SK-OV-3 cells using RNA polymerase I termination factor mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

Flow Cytometry: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Analysis of Hela cells using RNA polymerase I termination factor mouse mAb (green) and negative control (red).

Immunohistochemistry: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

Immunohistochemistry: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Analysis of paraffin-embedded cervical cancer tissues using RNA polymerase I termination factor mouse mAb with DAB staining.

Immunocytochemistry/Immunofluorescence: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

Immunocytochemistry/Immunofluorescence: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Analysis of MCF-7 cells using RNA polymerase I termination factor mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Applications for RNA polymerase I termination factor Antibody (2F4D8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNA polymerase I termination factor

The TTF1 gene encodes a 905 amino acid long, 103 kDA transcription termination factor 1 protein, also known as a RNA polymerase I termination factor, which functions in terminating ribosomal gene transcription. Additionally, it regulates fork arrest replication and RNA polymerase I transcription on chromatin. Through monitoring the activation as well as the silencing of rDNA transcription, TTF1 acts in rDNA regulation. TTF1 functions in RNA polymerase I transcription as well as mitochondrial transcription and gene expression. It is known to interact with genes PTRF, NFATC3, BAZ2A, SMAD1, and SMAD7. TTF1 has been researched regarding its role in lung cancer, small cell carcinoma, sex cord-gonadal stromal tumor, thyroiditis, blastoma, adenocarcinoma, desmoplastic small round cell tumors, and hirschsprung's disease.

Alternate Names

RNA polymerase I termination factor, transcription termination factor 1, Transcription termination factor I, transcription termination factor, RNA polymerase I, TTF-1, TTF-I

Gene Symbol

TTF1

Additional RNA polymerase I termination factor Products

Product Documents for RNA polymerase I termination factor Antibody (2F4D8) - BSA Free

Certificate of Analysis

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Product Specific Notices for RNA polymerase I termination factor Antibody (2F4D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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