RNase L Antibody (2E9) - BSA Free

Novus Biologicals | Catalog # NB100-351

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Hamster, Mouse (Negative), Primate, Rat (Negative)

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E9

Format

BSA Free
View all RNase L Primary Antibodies »

Product Specifications

Immunogen

Purified full-length recombinant human RNaseL produced in insect cells. [UniProt# Q05823]

Reactivity Notes

This antibody does not react with mouse or rat.

Localization

Cytoplasmic and Mitochondrial.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNase L Antibody (2E9) - BSA Free

Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]

Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]

RNase-L-Antibody-2E9-BSA-Free-Western-Blot-NB100-351-img0010.jpg
Immunohistochemistry: RNase L Antibody (2E9) - BSA Free [NB100-351]

Immunohistochemistry: RNase L Antibody (2E9) - BSA Free [NB100-351]

Immunohistochemistry: RNase L Antibody (2E9) [NB100-351] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain.
Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]

Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]

Western Blot: RNase L Antibody (2E9) [NB100-351] - RNaseL in insect cell extracts transfected with recombinant human RNase L (0.1 ug) and Hey1B (100 ug)
Knockout Validated: RNase L Antibody (2E9) - BSA Free [NB100-351]

Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351]

RNase-L-Antibody-2E9-BSA-Free-Knockout-Validated-NB100-351-img0009.jpg
RNase L Antibody (2E9) - BSA Free

Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] -

Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - dsRNA-induced SGs require G3BP1 & G3BP2, whereas RLBs do not.A, immunoblot for G3BP1, RNase L, GAPDH in parental (WT), RL-KO, G3BP-KO, & RL/G3BP-KO U-2 OS cells. B, immunoblot for G3BP1 & G3BP2 in RL-KO & RL/G3BP-KO U-2 OS cells. C, IF for G3BP1 & PABPC1 4 h post-poly(I·C) transfection or treatment with 500 μm sodium arsenite (SA) in the indicated cell lines. D, similar to C but enlarged to show RLB formation in U-2 OS-G3BP1/2-KO cells. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31896577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RNase L Antibody (2E9) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:1000-1:4000 (ECL)
Application Notes
This RNase L (2E9) antibody is useful for ELISA, Western Blot and Immunohistochemistry on paraffin-embedded sections. By WB, this antibody recognizes a band at ~83 kDa, representing RNase L. A low-molecular mass, 37 kDa, isoform of RNase L has been described in peripheral blood mononuclear cell extracts. In IHC-P, staining was observed in the cytoplasm of human colon cells. Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNase L

A virally induced endoribonuclease, RNase L is the terminal factor of the anti-viral action of interferon. Activation of RNase L results in inhibition of viral proliferation. Activation of RNase L has also been determined to degrade RNA, thus, initiating a cellular stress response, inducing apoptosis. Since mutations in RNase L relate to an increased incidence of prostate cancer, the activation of RNase L functions in counteracting prostate cancer, via apoptosis.

Alternate Names

2-5A-dependent ribonuclease, 2-5A-dependent RNase, 2'-5'-oligoisoadenylate synthetase-dependent, EC 3.1.26.-, HPC1, interferon-induced 2-5A-dependent RNase, MGC104972, MGC133329, PRCA1, prostate cancer 1, Ribonuclease 4, Ribonuclease L, ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent), RNase L, RNS4DKFZp781D08126

Gene Symbol

RNASEL

Additional RNase L Products

Product Documents for RNase L Antibody (2E9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RNase L Antibody (2E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RNase L Antibody (2E9) - BSA Free

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Protocols

View specific protocols for RNase L Antibody (2E9) - BSA Free (NB100-351):

RNase L Antibody (2E9):
Western Blot

1. Proteins are separated on a 10% SDS-PAGE gel.

2. Proteins are transferred to Immobilon-P membranes (Millipore Co.).

3. Following the protein transfer, the membrane is blocked with PBS-T [PBS + 0.07% Tween-20]+ 5% NFDM.

4. Anti-RNase L [cat# NB 100-351] is diluted 1:1,000, in blocking buffer and incubated for 2 hour at room temperature, gently shaking.

5. The membrane is then washed, 3 times with PBS-T, 5 minutes each.

6. Secondary antibody is incubated for 1 hour at room temperature, gently shaking.

7. The membrane is then washed, 4 times with PBS-T, 5 minutes each.

8. Membrane is developed using ECL reagents.


NOTE: Cell extracts of insect cells expressing human RNase L (0.1 ug) and Hey 1B (100 ug) were used as positive controls for this antibody.

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FAQs

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