ROCK2 Antibody (10E1) - BSA Free

Name: ROCK2 Antibody (10E1) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-15152
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15152

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10E1 expressed in HEK293

Format

BSA Free

View all ROCK2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ROCK2. UniProt ID (O75116).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROCK2 Antibody (10E1) - BSA Free

Western Blot: ROCK2 Antibody (10E1) [NBP3-15152]

Western Blot: ROCK2 Antibody (10E1) [NBP3-15152] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: ROCK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 161 KDa. Observed band size: 161 KDa.

Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (10E1) [NBP3-15152]

Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (10E1) [NBP3-15152] - Immunofluorescence staining of Hela Cells with at ROCK2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for ROCK2 Antibody (10E1) - BSA Free

Application

Recommended Usage

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ROCK2

ROCK2 is encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Alternate Names

ROCK-II, ROKalpha

Gene Symbol

ROCK2

Additional ROCK2 Products

Product Documents for ROCK2 Antibody (10E1) - BSA Free

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Certificate of Analysis

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Product Specific Notices for ROCK2 Antibody (10E1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.