RPA2 [p Ser4, p Ser8] Antibody - BSA Free

Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017]

Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Samples: Whole cell lysate (50 ug for WB in A; 1 mg for IP, 20% of IP loaded in B) from HeLa Cells that had been treated with EPE (+) or mock treated (-). In B, immunoprecipitated RPA32 was either treated with phosphatase (+) or mock treated (-). Antibodies: Phosphorylated RPA32 was detected using rabbit anti-phospho RPA32 Ser4/Ser8 antibody NBP1-23017 lot 2 at 1 ug/ml. Total RPA32 was detected using rabbit anti-RPA32 antibody NB100-332 at 1 ug/ml. RPA32 was immunoprecipitated using NB100-332 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds for phospho RPA32 Ser4/Ser8 (A and B) and 1 second for total RPA32 (A and B).

Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017]

Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows Westen Blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 ug/ml with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017]

Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of human breast carcinoma, MOCK treatment (left) and CIP treatment(right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) (used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017]

Immunoprecipitation: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with EPE (+) and/or Lambda PP (+) or mock treated (-). Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-RPA32 antibody NB100-332. For blotting immunoprecipitated Phospho RPA32 (S4/S8), rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 was used at 0.1 ug/ml. Lower Panel: Rabbit anti-RPA antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.

Western Blot: RPA2 [p Ser4, p Ser8] Antibody - BSA Free [NBP1-23017] -

Impact of HMGN1 knockdown on DNA damage response in lung adenocarcinoma. (A) Pearson correlation analysis showing the correlation between HMGN1 expression and RAD51 expression in LUAD. (B, C) RT–qPCR analysis of HMGN1 silencing in A549 and PC9 cells and its effect on RAD51 expression. (D, E) Western blot analysis of Rad51, p-RPA2, gamma H2AX and p-Chk1 proteins following HMGN1 depletion. (F) RT–qPCR analysis of RAD51 expression upon ectopic expression of HMGN1. (G) Western blot analysis of Rad51, p-RPA2, gamma H2AX and p-Chk1 proteins following HMGN1 overexpression. (G) Quantification of Gamma-H2AX fluorescence foci from three experiments shown as mean +/- SD. *p < 0.05, **p < 0.01,***p < 0.001, ns: not statistically significant. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-60352-8), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RPA2 [p Ser4, p Ser8] Antibody - BSA Free [NBP1-23017] -

Mechanism of resistance of ADR-Res cells to MK-8776ADR-Res cells were incubated with the indicated concentrations of either MK-8776, AZD1775 or PD166285 for 6 h, then analyzed by western blotting. These cells were only sensitive to PD166285 and only showed increased gamma H2AX and pHH3 at concentrations that inhibited pY15-CDK1/2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26595527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RPA2 [p Ser4, p Ser8] Antibody - BSA Free [NBP1-23017] -

Impact of HMGN1 knockdown on DNA damage response in lung adenocarcinoma. (A) Pearson correlation analysis showing the correlation between HMGN1 expression and RAD51 expression in LUAD. (B, C) RT–qPCR analysis of HMGN1 silencing in A549 and PC9 cells and its effect on RAD51 expression. (D, E) Western blot analysis of Rad51, p-RPA2, gamma H2AX and p-Chk1 proteins following HMGN1 depletion. (F) RT–qPCR analysis of RAD51 expression upon ectopic expression of HMGN1. (G) Western blot analysis of Rad51, p-RPA2, gamma H2AX and p-Chk1 proteins following HMGN1 overexpression. (G) Quantification of Gamma-H2AX fluorescence foci from three experiments shown as mean +/- SD. *p < 0.05, **p < 0.01,***p < 0.001, ns: not statistically significant. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-60352-8), licensed under a CC-BY license. Not internally tested by Novus Biologicals.