RPTOR Antibody (9N0A4)

Name: RPTOR Antibody (9N0A4)
Brand: Novus Biologicals
SKU: NBP3-16729
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16729

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9N0A4 expressed in HEK293

View all RPTOR Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1236-1335 of human RPTOR (Q8N122). VRIFDPRMPESVNVLQIVKGLTALDIHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

149 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPTOR Antibody (9N0A4)

Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729]

Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729] - Western blot analysis of extracts of various cell lines, using RPTOR Rabbit mAb (NBP3-16729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Immunohistochemistry-Paraffin: RPTOR Antibody (9N0A4) [NBP3-16729]

Immunohistochemistry-Paraffin: RPTOR Antibody (9N0A4) [NBP3-16729] - Immunohistochemistry of paraffin-embedded mouse kidney using RPTOR Rabbit mAb (NBP3-16729) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for RPTOR Antibody (9N0A4)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Simple Western

1:20 - 1:250

Western Blot

1:500 - 1:2000

Application Notes

See Simple Western Antibody Database for Simple Western validation: Tested in Mouse brain lysate, separated by Size, antibody dilution of 1;20, 1:100, 1:250, apparent MW was 152 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPTOR

Raptor (regulatory associated protein of TOR) is a conserved 150 kDa adaptor protein composed of a unique conserved region, three HEAT repeats and seven WD-40 repeats (1). Raptor recruits mTOR substrates S6K1 (p70 S6 kinase) and 4E-BP1, and regulates mTOR kinase activity (2-3). Even though Raptor is a positive regulator of mTOR, it has been shown that under nutrient deprivation, the Raptor-mTOR association is stabilized in a manner that inhibits mTOR kinase activity (1). Raptor association with mTOR is required for efficient S6K1 and 4E0BP1 phosphorylation (4).

Long Name

Regulatory Associated Protein of mTOR

Alternate Names

KIAA1303, Raptor

Gene Symbol

RPTOR

Additional RPTOR Products

Product Documents for RPTOR Antibody (9N0A4)

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RPTOR Antibody (9N0A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Interferons

Customer Reviews for RPTOR Antibody (9N0A4)

There are currently no reviews for this product. Be the first to review RPTOR Antibody (9N0A4) and earn rewards!

Have you used RPTOR Antibody (9N0A4)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image