RRBP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83957

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RRBP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELHSKLQSSEAEVRSKCEELSGLHGQLQEARAENSQLTERIRSIEALLEAGQARDAQDVQASQAEADQQQTRLKELESQVSGLEKEAIELREAVEQQKVKNNDLREK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RRBP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human colon, kidney, lymph node and placenta using Anti-RRBP1 antibody NBP1-83957 (A) shows similar protein distribution across tissues to independent antibody NBP1-83958 (B).
Immunocytochemistry/ Immunofluorescence: RRBP1 Antibody [NBP1-83957]

Immunocytochemistry/ Immunofluorescence: RRBP1 Antibody [NBP1-83957]

Immunocytochemistry/Immunofluorescence: RRBP1 Antibody [NBP1-83957] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human placenta using Anti-RRBP1 antibody NBP1-83957.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human skeletal muscle shows weak cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human kidney.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human colon.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human lymph node.
Simple Western: RRBP1 Antibody [NBP1-83957]

Simple Western: RRBP1 Antibody [NBP1-83957]

Simple Western: RRBP1 Antibody [NBP1-83957] - Simple Western lane view shows a specific band for RRBP1 in 0.2 mg/ml of h. Colon lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: RRBP1 Antibody [NBP1-83957]

Simple Western: RRBP1 Antibody [NBP1-83957]

Simple Western: RRBP1 Antibody [NBP1-83957] - Electropherogram image of the corresponding Simple Western lane view. RRBP1 antibody was used at 1:200 dilution on h. Colon lysate(s) respectively.
RRBP1 Antibody - BSA Free Immunohistochemistry-Paraffin: RRBP1 Antibody - BSA Free [NBP1-83957]

Immunohistochemistry-Paraffin: RRBP1 Antibody - BSA Free [NBP1-83957]

Analysis in human placenta and skeletal muscle tissues using NBP1-83957 antibody. Corresponding RRBP1 RNA-seq data are presented for the same tissues.
RRBP1 Antibody - BSA Free Western Blot: RRBP1 Antibody - BSA Free [NBP1-83957]

Western Blot: RRBP1 Antibody - BSA Free [NBP1-83957]

Analysis in human cell line CACO-2.

Applications for RRBP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Simple Western

1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in h. Colon lysate(s), separated by Size, antibody dilution of 1:200, apparent MW was 105 kDa.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RRBP1

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. (provided by RefSeq)

Alternate Names

180 kDa ribosome receptor homolog, DKFZp586A1420, ES/130, ES/130-related protein, ES130, FLJ36146, hES, KIAA1398, MGC157720, MGC157721, ribosome binding protein 1 (dog 180kD homolog), ribosome binding protein 1 homolog 180kDa (dog), Ribosome receptor protein, ribosome-binding protein 1, RRp

Gene Symbol

RRBP1

Additional RRBP1 Products

Product Documents for RRBP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RRBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RRBP1 Antibody - BSA Free

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Protocols

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